| Project/Area Number |
10470169
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Osaka City University |
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Principal Investigator |
YOSHIKAWA Junichi Department of Internal Medicine and Cardiology, Osaka City University School of Medicine, Professor and Chairman, 医学部, 教授 (60275245)
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| Co-Investigator(Kenkyū-buntansha) |
OMURA Takashi Department of Internal Medicine and Cardiology, Osaka City University, School of Medicine, Assistant Professor, 医学部, 助手 (70295707)
YOSHIYAMA Minoru Department of Internal Medicine and Cardiology, Osaka City University, School of Medicine, Associate Professor, 医学部, 講師 (30240956)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 2000: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1999: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1998: ¥8,800,000 (Direct Cost: ¥8,800,000)
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| Keywords | Myocardium / Ischemia / Echocardiography / gene expression / Ischemia / Echocardiography / Myocardiam / Echo cardiography / Gene expression |
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| Research Abstract |
Recently, microbubble can be used as a contrast agent and We try to detect the myocardial perfusion. We also try to make angiogenesis by gene therapy. The purpose of this study is to make angiogenesis by gene therapy and angiogenesis can be evaluated by contrast agent. In the first year, we succeeded to hace myocardila infarcted model in rat and mice. We analyzed signal transduction and mRNA expressions after myocardial infarction and evaluated morphological change and cardiac function by echocardiogram. In the second year, we could get gene transfer method by adeno virus vector and myocardial perfusion could be evaluated by contrast echocardiography. In the last year, we try to transfer gene to the myocardium in vivo model. This protocol is now on going. In clinical, we can detect myocardial perfusion by echo-contrast agent.
In experimental and clinical research, we will detect microcirculation and collateral flow in the myocardium near future.
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