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Analysis of the molecular mechanism regulating the development of hematopoietic stem cells in cord blood and their application to ex vivo expansion

Research Project

Project/Area Number 10470174
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionThe University of Tokyo

Principal Investigator

TSUJI Kohichiro  The Institute of Medical Science, The University of Tokyo Associate Professor, 医科学研究所, 助教授 (50179991)

Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1998: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordscord blood / ex vivo expansion / stem cell factor / Flk2 / Flt3 ligand / thrombopoietin / interleukin-6 / solble IL-6 receptor / stem cell factor / Soluble IL-6 receptor / 臍帯血 / 造血幹細胞 / 造血前駆細胞 / NOD / SCIDマウス / 胎生期造血 / AGM領域 / 造血幹細胞移植
Research Abstract

We demonstrated a significant ex vivo expansion of cord blood hematopoietic stem cells capable of repopulating in NOD/SCID mice, using a combination of stem cell factor (SCF), Flk2/Flt3 ligand (FL), thrombopoietin (TPO) and a complex of interleukin (IL)-6 and soluble IL-6 receptor (IL-6/sIL-6R). When 1 to 2x104 cord blood CD34+ cells and the cells cultured with SCF+FL, SCF+FL+TPO and SCF+FL+TPO+IL-6/sIL-6R for 7 days were transplanted into NOD/SCID mice, 6/25 (24%), 7/16 (44%), 7/14 (50%) and 13/16 (81%) recipients revealed successful engraftment, and the bone marrow cells of the engrafted recipients contained 1.4%, 2.3%, 6.7% and 11.5% of human CD45+ cells, respectively, 10 to 12 weeks after the transplantation. The CD45+ cells in the recipients engrafted with the cells cultured with SCF+FL+TPO+IL-6/sIL-6R consisted of various lineages and a large number of CD34+ cells, which formed human hematopoietic colonies in in vitro clonal culture. In comparion of the reconstitution between fresh CD34+ cells from 12 samples and their progenies cultured with SCF+FL+TPO+IL-6/sIL-6R, the proportion of CD45+ cells in recipient marrow was 10 times higher in the latter, indicating the significant expansion of the long-term repopulating hematopoietic stem cells. The expansion rate was estimated at 4.2-fold by a limiting dilution method. The addition of IL-3 to the cytokine combination abrogated the repopulating ability of the expanded cells. The present study may provide a novel culture method for the expansion of human transplantable hematopoietic stem cells aimed for clinical applications.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (140 results)

All Other

All Publications (140 results)

  • [Publications] Ueda T.: "Expansion of human NOD/SCID-repopulating cells by a combination of stem cell factor, Flk2/Flt3 ligand, thrombopoietin and a complex of interleukin-6 and soluble interleukin-6 receptor."J.Clin.Invest.. 105. 1013-1021 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hayashi Y.: "SN-1,a novel leukemic cell line with t (11;16) (q23; p13): myeloid characteristics and resistance to retinoids and vitamin D3."Cancer Res.. 60. 1139-1145 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ebihara Y: "Exclusive expression of G-CSF receptor on myeloid progenitors in bone marrow CD34^+ cells."Br.J.Haematol.. 109. 153-161 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ueda T.: "Hematopoietic repopulation ability of cord blood CD34^+ cells in NOD/Shi-scid mice."Stem Cells. 18. 204-213 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ueda T.: "Massive pericardial and pleural effusion with anasarca following allogeneic bone marrow transplantation."Int.J.Hematol.. 71. 394-397 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sato T.: "Erythroid progenitors differentiate and mature in response to endogenous erythropoietin."J.Clin.Invest.. 106. 263-270 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ishii T.: "Improvement in bronchiolitis obliterans organizing pneumonia by combination of oral predonisolone and low dose erythromycin in a child after allogegeneic bone marrow transplantation."Bone Marrow Transplant. 26. 907-910 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sugiyama H.: "Peroxisome proliferator-activated receptors are expressed in mouse bone marrow-derived mast cells."FEBS Lett.. 467. 259-262 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshino H.: "Natural killer cell depletion by anti-asialo GM1 antiserum treatment enhances human hematopoietic stem cell engraftment in NOD/Shi-scid mice."Bone Marrow Transplant.. 26. 1211-1216 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takaki S.: "Control of B cell production by the adaptor protein Lnk, a negative regulator of tyrosine kinase signaling."Immunity. 13. 599-609 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsui A.: "gp130 and c-kit signals synergistically induce thrombopoietin production by hematopoietic cells."Int.J.Hematol.. 72. 455-462 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tsuji K.: "Expression of G-CSF receptor on myeloid progenitors."Leukemia and Lymphoma. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsuoka S.: "CD 34 expression on long-term repopulating hematopoietic stem cells changes during developmental stages."blood. 97. 419-425 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ma F.: "Cytokine requirement for the development of T-lineage potential from lymphohematopoietic progenitor cells."Br.J. Haematol.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sugiyama H.: "Peroxisome proliferator-activated receptors are expressed in human cultured mast cells : a possible role of these receptors in negative regulation of mast cell activation."Eur.J.Immunol.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Xu M.: "Evidence for the presence of primitive megakaryopoiesis in the early yolk sac."Blood. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ueda T.: "Hematopoietic capability of cord blood CD34^+ cells : a comparison with that of adult bone marrow CD34^+ cells."Int.J.Hoematol.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshimasu T.: "Prompt and durable hematopoietic reconstitution by an unrelated cord blood transplantation in a child with Fanconi anemia."Bone Marrow Transplant.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyamoto K.: "Inhibitory effect of interleukin-3 on the early development of human B-lymphopoiesis."Br.J.Haematol.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsuoka S.: "Generation of definitive hematopoietic stem cells from murine early yolk sac and paraaortic splanchnopleura by AGM region-derived stromal cells."Blood. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hisakawa H.: "Human granulocyte-macrophage colony-stimulating factor (hGM-CSF) stimulates primitive and definitive erythropoiesis in mouse embryo expressing hGM-CSF receptors but not erythropoietin receptors."Blood. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ma F.: "Development of human lymphohematopoietic stem/progenitor cells defined by CD34 and CD81 expressions."Blood. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 石井武文: "急性リンパ性白血病治療後期に発症したL-アスパラギナーゼによる壊死性膵炎"日小血会誌. 14. 328-332 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 岩本彰太郎: "大量rhG-CSF療法中にmonosomy7を伴うMDSに移行したKostmann症候群"日小血会誌. 14. 338-345 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 三牛哲夫: "造血幹細胞移植における支持療法と今後の課題"Biotherapy. 14. 292-300 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 辻浩一郎: "増幅造血幹細胞移植ガイドライン"分子細胞療法. 1. 197-201 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 海老原康博: "造血幹細胞の同定検査 2)コロニー法"検査と技術. 28. 971-972 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 辻浩一郎: "造血幹細胞の起源と性状"医学のあゆみ. 194. 1021-1025 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 杉山大介: "AGM領域"分子細胞療法. 1. 92-93 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 辻浩一郎: "造血幹細胞の増幅"血液・免疫・腫瘍. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 辻浩一郎: "試験管内造血幹細胞増幅"現代医療. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 辻浩一郎: "造血幹細胞コロニー刺激因子と臍帯血造血幹細胞の体外増幅"日本産婦人科・新生児血液学会. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakahata T.: "Cell Therapy"Springer-Verlag, Tokyo. 2 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakahata T.: "Mast Cell Biology"Academic Press (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tsuji K.: "Methods in Molecular Medicine-Cytokines and Colony Stimulating Factors"The Human Press (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 田中竜平: "造血器疾患治療マニュアル"メディカルレビュー社(大阪)(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 辻浩一郎: "小児の症候群"診断と治療社(東京)(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ueda Y: "Expansion of human NOD/SCID-repopulating cells by a combination of stem cell factor, Flk2/Flt3 ligand, thrombopoietin and a complex of interleukin-6 and soluble interleukin-6 receptor."J Clin Invest. 105. 1013-1021 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hayashi Y: "SN-1, a novel leukemic cell line with t (11 ; 16)(q23 ; p13) : myeloid characteristics and resistance to retinoids and vitamin D3."Cancer Res. 60. 1139-1145 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ebihara Y: "Exclusive expression of G-CSF receptor on myeloid progenitors in bone marrow CD34^+ cells."Br J Haematol. 109. 153-161 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ueda T: "Hematopoietic repopulating ability of cord blood CD34^+ cells in NOD/Shi-scid mice."Stem Cells. 18. 204-213 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ueda T: "Massive pericardial and pleural effusion with anasarca following allogeneic bone marrow transplantation."Int J Hematol. 71. 394-397 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sato T: "Erythroid progenitors differentiate and mature in response to endogenous erythropoietin."J Clin Invest. 106. 263-270 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ishii T: "Improvement in bronchiolitis obliterans organizing pneumonia by combination of oral predonisolone and low dose erythromycin in a child after allogegeneic bone marrow transplantation."Bone Marrow Transplant. 26. 907-910 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sugiyama H: "Peroxisome proliferator-activated receptors are expressed in mouse bone marrow-derived mast cells."FEBS Lett. 467. 259-262 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshino H: "Natural killer cell depletion by anti-asiale GM1 antiserum treatment enhances human hematopoietic stem cell engraftment in NOD/Shi-scid mice."Bone Marrow Transplant. 26. 1211-1216 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takaki S: "Control of B cell production by the adaptor protein Lnk, a negative regulator of tyrosine kinase signaling."Immunity. 13. 599-609 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsui A: "gp130 and c-kit signals synergistically induce thrombopoietin production by hematopoietic cells."Int J Hematol. 72. 455-462 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tsuji K: "Expression of G-CSF receptor on myeloid progenitors."Leukemia and Lymphoma. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsuoka S: "CD34 expression on long-term repopulating hematopoietic stem cells changes during developmental stages."Blood. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ma F: "Cytokine requirement for the development of T-lineage potential from lymphohematopoietic progenitor cells."Br J Haematol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sugiyama H: "Peroxisome proliferator-activated receptors are expressed in human cultured mast cells : a possible role of these receptors in negative regulation of mast cell activation."Eur J Immunol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Xu M: "Evidence for the presence of primitive megakaryopoiesis in the early yolk sac."Blood. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ueda T: "Hematopoietic capability of cord blood CD34^+ cells : a comparison with that of adult bone marrow CD34^+ cells."Int J Hamatol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshimasu T: "Prompt and durable hematopoietic reconstitution by an unrelated cord blood transplantation in a child with Fanconi anemia."Bone Marrow Transplant. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyamoto K: "Inhibitory effect of interleukin-3 on the early development of human B-lymphopoiesis."Br.J.Haematol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsuoka S: "Generation of definitive hematopoietic stem cells from murine early yolk sac and paraaortic splanchnopleura by AGM region-derived stromal cells."Blood. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hisakawa H: "Human granulocyte-macrophage colony-stimulating factor (hGM-CSF) stimulates primitive and definitive erythropoiesis in mouse embryo expressing hGM-CSF receptors but not erythropoietin receptors."Blood. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ma F: "Development of human lymphohematopoietic stem/progenitor cells defined by CD34 and CD81 expressions."Blood. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakahata T: "Ex vivo expansion of human hematopoietic stem cells."Cell Therapy. Springer-Verlag, Tokyo. 105-106 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tsuji K: "Protocol for cytokine mediated expansion of human NOD/SCID-repopulating cells."Methods in Molecular Medicine-Cytokines and Colony Stimulating Factors. The Human press, Totowa (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakahata T: "Cytokine regulation of human mast cells."Mast Cell Biology. Academic Press (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ueda T.: "Expansion of human NOD/SCID-repopulating cells by a combination of stem cell factor, Flk2/Flt3 ligand,thrombopoietin and a complex of interleukin-6 and soluble interleukin-6 receptor."J.Clin.Invest.. 105. 1013-1021 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hayashi Y.: "SN-1, a novel leukemic cell line with t(11;16)(q23;p13): myeloid characteristics and resistance to retinoids and vitamin D3."Cancer.Res.. 60. 1139-1145 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ebihara Y.: "Exclusive expression of G-CSF receptor on myeloid progenitors in bone marrow CD34^+ cells."Br.J.Haematol.. 109. 153-161 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ueda T.: "Hematopoietic repopulating ability of cord blood CD34^+ cells in NOD/Shi-scid mice."Stem Cells. 18. 204-213 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ueda T.: "Massive pericardial and pleural effusion with an asarca following allogeneic bone marrow transplantation."Int.J.Hematol.. 71. 394-397 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sato T.: "Erythroid progenitors differentiate and mature in response to endogenous erythropoietin."J.Clin.Invest.. 106. 263-270 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ishii T.: "Improvement in bronchiolitis obliterans organizing pneumonia by combination of oral predonisolone and low dose erythromycin in a child after allogegeneic bone marrow transplantation."Bone Marrow Transplant. 26. 907-910 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sugiyama H.: "Peroxisome proliferator-activated receptors are expressed in mouse bone marrow-derived mast cells."FEBS Lett.. 467. 259-262 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshino H.: "Natural killer cell depletion by anti-asialo GM1 antiserum treatment enhances human hematopoietic stem cell engraftment in NOD/Shi-scid mice."Bone Marrow Transplant.. 26. 1211-1216 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takaki S.: "Control of B cell production by the adaptor protein Lnk, a negative regulator of tyrosine kinase signaling."Immunity. 13. 599-609 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Matsui A.: "gp130 and c-kit signals synergistically induce thrombopoietin production by hematopoietic cells."Int.J.Hematol.. 72. 455-462 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tsuji K.: "Expression of G-CSF receptor on myeloid progenitors."Leukemia and Lymphoma. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Matsuoka S.: "CD34 expression on long-term repopulating hematopoietic stem cells changes during developmental stages."Blood. 97. 419-425 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ma F.: "Cytokine requirement for the development of T-lineage potential from lymphohematopoietic progenitor cells."Br.J.Haematol.. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Sugiyama H.: "Peroxisome proliferator-activated receptors are expressed in human cultured mast cells : a possible role of these receptors in negative regulation of mast cell activation."Eur.J.Immunol.. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Xu M.: "Evidence for the presence of primitive megakaryopoiesis in the early yolk sac."Blood. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Ueda T.: "Hematopoietic capability of cord blood CD34^+ cells : a comparison with that of adult bone marrow CD34^+ cells."Int.J.Hoematol.. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshimasu T.: "Prompt and durable hematopoietic reconstitution by an unrelated cord blood transplantation in a child with Fanconi anemia."Bone Marrow Transplant.. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Miyamoto K.: "Inhibitory effect of interleukin-3 on the early development of human B-lymphopoiesis."Br.J.Haematol.. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Matsuoka S.: "Generation of definitive hematopoietic stem cells from murine early yolk sac and paraaortic splanchnopleura by AGM region-derived stromal cells."Blood. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Hisakawa H.: "Human granulocyte-macrophage colony-stimulating factor (hGM-CSF) stimulates primitive and definitive erythropoiesis in mouse embryo expressing hGM-CSF receptors but not erythropoietin receptors."Blood. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Ma F.: "Development of human lymphohematopoietic stem/progenitor cells defined by CD34 and CD81 expressions."Blood. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] 石井武文: "急性リンパ性白血病治療後期に発症したL-アスパラギナーゼによる壊死性膵炎"日小血会誌. 14. 328-332 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 岩本彰太郎: "大量rhG-CSF療法中にmonosomy7を伴うMDSに移行したKostmann症候群"日小血会誌. 14. 338-345 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 三井哲夫: "造血幹細胞移植における支持療法と今後の課題"Biotherapy. 14. 292-300 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 辻浩一郎: "増幅造血幹細胞移植ガイドライン"分子細胞療法. 1. 197-201 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 海老原康博: "造血幹細胞の同定検査 2)コロニー法"検査と技術. 28. 971-972 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 辻浩一郎: "造血幹細胞の起源と性状"医学のあゆみ. 194. 1021-1025 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 杉山大介: "AGM領域"分子細胞療法. 1. 92-93 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 辻浩一郎: "造血幹細胞の増幅"血液・免疫・腫瘍. (印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] 辻浩一郎: "試験管内造血幹細胞増幅"現代医療. (印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] 辻浩一郎: "造血幹細胞コロニー刺激因子と臍帯血造血幹細胞の体外増幅"日本産婦人科・新生児血液学会. (印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakahata T.: "Cell Therapy"Springer-Verlag, Tokyo. 2 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakahata T.: "Mast Cell Biology"Academic Press (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Tsuji K.: "Methods in Molecular Medicine-Cytokines and Colony Stimulating Factors"The Human Press (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] 田中竜平: "造血器疾患治療マニュアル"メディカルレビュー社(大阪)(印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] 辻浩一郎: "小児の症候群"診断と治療社(東京)(印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] Sui X,Tsuji K,Ebihara Y,Kishimoto T.Nakahara T: "Soluble IL-6 receptor with IL-6 stimulates megakaryopoiesis from human CD34^+ cells through gp130 signaling"Blood. 93. 2525-2532 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Honda H,Tsuji K,Yazaki Y,Hirai H: "Expression of E2A-HLF chimertic protein induced T-cell apoptosis,B-cell maturation arrest,and development of acute lymp"Blood. 93. 2780-2790 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kinoshita T,Tsuji K,Nakahara T,Miyajima A: "Hepatic differentiation induced by oncostatin M attenuates fetal liver hematopoiesis"Proc Natl Acad Sci USA. 96. 7265-7270 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yang F,Tsuji K,Watanabe S,: "Differential effects of human granulocyte colony-stimulating factor(hG-CSF) and thrombopoietin on megakaryopoiesis and platelet function in hG-CSF receptor-transgenic mice"Blood. 94. 950-958 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsumoto T,Tsuji K,Nakahara T,Yoshimura A: "Suppression of STAT5 functions in liver,mammary glands, and T cells in cytokine-inducible SH2-containing protein 1 transgenic mice"Mol Cell Biol. 19. 6396-6407 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ishii T,Tsuji K,Nakahata T,Maekawa T: "Expression of stomal cell-derived factor-1/pre-B cell growth -stimulating factor receptor,CXC chemokine receptor 4,on CD34^+ human bone marrow cells is a phenotypic alteration for committed lymphoid progenitors"J Immunol. 163. 3612-3620 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsuoka S,Tsuji K,Kikuchi A,Nakahara T: "CD34 expression on long-term repopulating hematopoietic stem cells changes during developmental stages"Blood. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Ebihara Y,Xu M-j Nakahara T,Tsuji K: "Exclusive expression of G-CSF receptor on myeloid progenitor in bone marrow CD34^+cells"Br J Haematol. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Hayashi Y,Honma Y,Tsuji K,Nakahata T: "SN-1,a novel leukemic cell line with t(11;16)(q23;p13): myeloid characteristics and resistance to retinoids and vitamin D3"Cancer Res. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Ueda Y,Tsuji K,Tanaka R,Kobayashi K: "Expansion of human NOD/SCID-repopulating cells by a combination of stem cell factor,Flk2/Flt3 ligand,thrombopoiet receptor"J Clin Invest. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Ueda T,Manabe A,Tsuji K,: "Massive pericardial and pleural effusion with anasarca following allogeneic bone marrow transplantation"Int J Hematol. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Sugiyama H,Tsuji K,Nakahata T: "Peroxisome proliferator-activated receptor are expressed in mouse bone marrow-derived mast cells"FEBS Lett. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Xu M,Matsuoka S,Nakahata T,Tsuji K: "Evidence for presence of primitive megakaryopoiesis in the early yolk sac"Blood. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Nakahata T,Toru H,Tsuji K: "Cytokine regulation of human mast cells"Marone G,Lichtenstein LM,Galli SJ(eds),Academic Press,. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] 辻浩一郎: "血液内科学、造血調節機構と造血微小環境"浅野茂隆編、中外医学社(東京). 6 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 辻浩一郎: "内科学教科書、造血幹細胞の性状"黒川清、松澤祐次編、文光堂(東京). 3 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 辻浩一郎: "造血器疾患治療マニュアル、造血器疾患の幹細胞移植"浅野茂隆、池田康夫、内田卓編、メディカルレビュー社(印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] Mukouyama Y: "In vitro expansion of murine multipotential hematopoietic progenitors from the embryonic aorta-gonad-mesonephros region." Immunity. 8. 105-114 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nishihara M: "A combination of stem cell factor and granulocyte colony-stimulating factor enhances proliferation and differentiation of human progenitor B cell supported by murine stromal cell line MS-5." Eur.J.Immunol.28. 855-864 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsuji K: "Interferon-γ and human megakaryocyte progenitors." Leukemia and Lymphoma. 31. 107-113 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Maruyama K: "Characterization of peripheral blood progenitor cells mobilized by nartograstim(N-terminal replaced granulocyte colony-stimulating factor)in normal volunteers." Bone Marrow Transplant.22. 313-320 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takahashi T: "A potential molecular approach to ex vivo expansion with recombinant EGFR-expressing adenovirus vector." Blood. 91. 4509-4515 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sato T: "Induction of the erythropoietin receptor gene and acquisition of responsiveness to erythropoietin by stem cell factor in HML/SE, a human leukemic cell line." J.Biol.Chem.273. 16921-16926 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Xu M: "Stimulation of mouse and human primitive hematopoiesis by murine embryonic aorta-gonad-mesonephros-derived stromal cell lines." Blood. 92. 2032-2040 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yang F-C: "Human G-CSF stimulates the development ofprimitive multipotential progenitors of human G-CSF receptor-transgenic mice, but dose not affect their commitment in vitro and in vivo." Blood. 92. 4632-4640 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kempuraj D: "Characterization of mast cell-committed progenitors present in human umbilical cord blood." Blood. (発表予定).

    • Related Report
      1998 Annual Research Report
  • [Publications] Sui X: "Soluble IL-6 receptor with IL-6 stimulates megakaryopoiesis from human CD34^+ cells through gp130 signaling." Blood. (発表予定).

    • Related Report
      1998 Annual Research Report
  • [Publications] Yang F-C: "Effects of human granulocyte colony-stimulating factor(hG-CSF)on megakaryopoiesis and platelet function in hG-CSF receptor-transgenic mice." Blood. (発表予定).

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamada K: "A novel strategy for retrovirus-mediated gene transfer into human primitive hematopoietic cells using IL-6/soluble IL-6 receptor complex and stem cell factor." Hum.Gene Ther.(発表予定).

    • Related Report
      1998 Annual Research Report
  • [Publications] 菊地 陽: "Phl陽性急性リンパ性白血病の骨髄移植における多分割高線量の全身照射の試み" 日本小児科血液学会雑誌. 12. 452-456 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 辻 浩一郎: "造血幹細胞の起源と性状" Molecular Medicine. 35. 157-164 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 辻 浩一郎: "AGM(acrta-gonad-mescnephros)領域と造血幹細胞" 臨床免疫. 30. 1278-1283 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 辻 浩一郎: "末梢血幹細胞の性状と動員のメカニズム" BIO Crinica. 13. 808-812 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 辻 浩一郎: "末梢血幹細胞の性状" 日常診療と血液. 8. 1247-1253 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 辻 浩一郎: "臍帯血幹細胞の特性" 低温医学. 24. 161-165 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 辻 浩一郎: "AGM領域の造血幹細胞とストローマ細胞" 臨床血液. (発表予定).

    • Related Report
      1998 Annual Research Report
  • [Publications] 辻 浩一郎: "ex vivo増幅の現状と展望" 実験医学. (発表予定).

    • Related Report
      1998 Annual Research Report
  • [Publications] 菊地 陽: "末梢血造血幹細胞の採取・凍結保存の標準的手法と幹細胞検定法" 低温医学. (発表予定).

    • Related Report
      1998 Annual Research Report
  • [Publications] 辻 浩一郎: "血液疾患" 医歯薬出版株式会社, 628 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 辻 浩一郎: "血液内科学" 中外医学社(出版予定),

    • Related Report
      1998 Annual Research Report
  • [Publications] 辻 浩一郎: "内科学教科書" 文光堂(出版予定),

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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