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Development of novel mouse model for autoimmune diseases using autoantigen knockout mice

Research Project

Project/Area Number 10470189
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionKeio University

Principal Investigator

NISHIKAWA Takeji  Keio Univ. School of Medicine, Dept.of Dermatology, Professor, 医学部, 教授 (50051579)

Co-Investigator(Kenkyū-buntansha) KOYASU Shigeo  Keio Univ. School of Medicine, Dept.of Dermatology, Professor, 医学部, 教授 (90153684)
MASAYUKI Amagai  Keio Univ. School of Medicine, Dept.of Dermatology, Assistant Professor, 医学部, 専任講師 (90212563)
SHIMIZU Hiroshi  Keio Univ. School of Medicine, Dept.of Dermatology, Associate Professor, 医学部, 助教授 (00146672)
SUZUKI Harimi  Keio Univ. School of Medicine, Dept.of Dermatology, Assistant Professor, 医学部, 専任講師 (70235985)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 1999: ¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1998: ¥7,900,000 (Direct Cost: ¥7,900,000)
KeywordsAutoimmune diseases / Pemphigus / Animal model / Desmoglein / Tolerance / Autoantibody / B cell / Knochout mouse
Research Abstract

The development of experimental models of active autoimmune diseases can be difficult due to tolerance of autoantigens. Knockout mice do not acquire tolerance of the defective gene product. Using knockout mice lacking desmoglein 3 (Dsg3), the target antigen of pemphigus vulgaris (PV), we established a method to generate an active disease model for this autoantibody-mediated disease. Dsg3ィイD1-/-ィエD1 mice, but not Dsg3ィイD1+/-ィエD1 littermates, produced anti-Dsg3 IgG that was able to bind the native Dsg3 when immunized with recombinant mouse Dsg3. Splenocytes from the immunized Dsg3ィイD1-/-ィエD1 mice were then adoptively transferred into Rag-2ィイD1-/-ィエD1 immunodeficient mice expressing Dsg3. Anti-Dsg3 IgG was stably produced in the recipient mice for over 6 months without further boosting. This IgG bound to Dsg3 in vivo and disrupted the cell-cell adhesion of keratinocytes. Consequently, the recipient mice developed erosions in their oral mucous membranes with typical histologic findings of PV. In addition, the recipient mice showed telogen hair loss, as found in Dsg3ィイD1-/-ィエD1 mice. Collectively, the recipient mice developed the phenotype of PV due to the pathogenic anti-Dsg3 IgG. This model will be valuable for developing novel therapeutic strategies. Furthermore, our approach can be applied broadly for the development of various autoimmune disease models.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Mahoney MG, Wang Z, Rothenberger KL, Koch PJ, Amagai M, Stanley JR: "Explanation of the clinical and microscopic localization of lesions in pemphigus foliaceus and vulgaris"J Clin Invest. 103. 461-468 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Wu H, Wang ZH, Yan A, Lyle S, Fakhazadeh S, Wahl JK, Wheelock MJ, Uitto J, Amagai M, Stanley JR.: "Protection of neonates against pemphigus foliaceus by desmoglein3"N Eng. J. Med. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishii K, Amagai M, Komai A, et al: "Desmoglein 1 and desmoglein 3 and the target arutoantigens in herpetiform pemphigus"Arch Dermatol.. 135. 943 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nishifuji K, Amagai M, Kuwana M, Iwasaki T, Nishikawa T: "Detection of antigen-specific B cells in patients with pemphigus vulgaris by enzyme-linked immunospot(ELISPOT) Assay: requirement of T cell collaboration for autoantibody production"J Invest Dermatol. 114. 88-94 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Proby CM, Ohta T, Suzuki H, et al: "Development of chimeric molecules for recognition and targeting of antigen-specific B cells in pemphigus vulgaris"Br J Dermatol. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Amagai M, Tsunoda K, Suzuki H, Nishifuji K, Koyasu S, Nishikawa T: "Use of autoantigen knockout mice to develop an active autoimmune disease model of permphigus"J Clin Invest. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Amagai M: "Advances in pemphigus foliaceus and pemphigus vulgaris"In D. Dyall-Smith & R. Marks(Eds.), Dermatology at Millennium: Proceeding of 19th World Congress of Dermatology New York: Partheonon Publishing. 812 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mahoney MG, Wang Z, Rothenberger KL, Koch PJ, Amagai M, Stanley JR.: "Explanation for the clinical and microscopic localization of lesions in pemphigus foliaceus and vulgaris."J Clin Invest. 103. 461-468 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishii K, Amagai M, Komai A, Ebihara T, Chorzelski, TP, Jablonska S, Ohya K, Nishikawa T, Hashimoto T.: "Desmoglein 1 and desmoglein 3 are the target autitigens in herpetiform pemphigus."Arch Dermaltol. 135. 943-947 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Proby CM, Ohta T, Suzuki H, Koyasu S, Gamou S, Shimizu N, Wheelock MJ, Nishikawa T, Amagai M.: "Development of chimeric molecules for recognition and targeting of antigen-specific B cells in pemphigus vulgaris."Br J Dermaltol. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nishifuji K, Amagai M, Kuwana M, Iwasaki T, Nishikawa T.: "Detection of antigen-ispecific B cells in patients with pemphigus vulgaris by enzyme-linked immunospot (ELISPOT) Assay: requirement of T cell collaboration for autoantibody production."J Invest Dermatol. 114. 88-94 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Amagai M,Tsunoda K, Suzuki H, Nishifuji K, Koyasu S, Nishikawa T.: "Use of autoantigen knockout mice to develop an active autoimmune disease model of pemphigus."J Clin Invest. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Wu H, Wang ZH, Yan A, Lyle S, Fakharzadeh S, Wahl JK, Wheelock MJ, Uitto J, Amagai M, Stanley JR.: "Protection of neonates against pemphigus foliaceus by desmoglein 3."N Eng J Med. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mahoney MG,Wang Z,Rothenberger KL,Koch PJ,Amagai M,Stanley JR.: "Explanation for the clinical and microscopic localization of lesions in pemphigus foliaceus and vulgaris"J Clin Invest. 103. 461-468 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Wu H,Wang ZH,Yan A,Lyle S,Fakharzadeh S,WahlJK,Wheelock MJ,Uitto J,Amagai M,Stanley JR.: "Protection of neonates against pemphigus foliaceus by desmoglein 3"N Eng J Med. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ishii K,Amagai M,Komai A,et al.: "Desmoglein 1 and desmoglein 3 are the target autoantigens in herpetiform pemphigus"Arch Dermatol. 135. 943-947 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nishifuji K,Amagai M,Kuwana M,Iwasaki T,Nishikawa T: "Detection of antigen-specific B cells in patients with pemphigus vulgaris by enzyme-linked immunospot(ELISPOT)Assay:requirement of T cell collaboration for autoantibody production"J Invest Dermatol. 114. 88-94 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Proby CM,Ohta T,Suzuki H,et al.: "Development of chimeric molecules for recognition and targeting of antigen-specific B cells in pemphigus vulgaris"Br J Dermatol. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Amagai M,Tsunoda K,Suzuki H,Nishifuji K,Koyasu S,Nishikawa T: "Use of autoantigen knockout mice to develop an active autoimmune disease model of pemphigus"J Clin Invest. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Amagai M: "Advances in pemphigus foliaceus and pemphigus vulgaris"In D.Dyall-Smith&R.Marks(Eds.),Dermatology at Millennium:Proceeding of 19th World Congress of Dermatology New York:Parthenon Publishing. 812 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hertl M, Karr RW,Amagai M,Katz SI: "Heterogeneous MHC II restriction pattern of autoreactive desmoglein 3 specific T cell responses in pemphigus vulgaris patients and normals" J Invest Dermatol. 110. 388-392 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Amagai M, Nishikawa T, Nousari HC,Anhalt GJ, Hashimoto T: "Antibodies against desmoglein 3 (pemphigus vulgaris antigen) are present in sera from patients with paraneoplastic pemphigus and cause acantholysis in vivo in neonatal mice" J Clin Invest. 102. 775-782 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Amagai M, Tsunoda K, Zillikens D, Nagai T, Nishikawa T: "The clinical phenotype of pemphigus is defined by the anti-desmoglein autoantibody profile" J Am Acad Dermatol. 40. 167-170 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Proby C, Fujii Y, Owaribe K, Nishikawa T,Amagai M: "Human autoantibodies against HD1/plectin in paraneoplastic pemphigus" J Invest Dermatol. 112. 153-156 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mahoney MG, Wang Z, Rothenberger KL, Koch Pj,Amagai M, Stanley JR: "Explanation for the clinical and microscopic localization of lesions in pemphigus foliaceus and vulgaris" J Clin Invest. 103. 461-468 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Amagai M, Komai A, Hashimoto T, Shirakata Y, Hashimoto K, Yamada T, Kitajima Y, Ohya K, Iwanami H, Nishikawa T: "Usefulness of enzyme-linked immunosorbent assay(ELISA)using recombinant desmogleins 1 and 3 for serodiagnosis of pemphigus" Br J Dermatol. 140. 351-357 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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