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The Basic Study of Multidisciplinary Treatment for Malignant Tumors using MnSOD Gene Therapy

Research Project

Project/Area Number 10470196
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

KURODA Masahiro  Medical School, Hospital, OKAYAMA UNIVERSITY, Lecturer, 医学部・附属病院, 講師 (50225306)

Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2000: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥2,800,000 (Direct Cost: ¥2,800,000)
KeywordsMnSOD / gene therapy / radiotherapy / hyperthermia / chemotherapy / tumorigenicity / apoptosis / multidisciplinary treatment / MnSOD / 科学療法
Research Abstract

1) We confirmed that MnSOD gene overexpression increased the in vivo effects of radiotherapy, hyperthermia, and chemotherapy, using MnSOD gene-tranfected fibrosarcoma cell line, FSa-II.
2) Experiments using scid mice revealed that overexpression of MnSOD gene suppressed spontaneous apoptosis without a resultant alteration in in vivo growth of the mouse fibrosarcoma, FSa-II.
3) Newly developed microscopic system was available to indicate that MnSOD gene overexpression increased apoptosis after hyperthermic treatments.
4) In vitro timelapse microscopic observation of cellular moving ability did not show any difference by MnSOD gene overexpression.
5) Intracellular MnSOD activity following gene-transfection by in vivo electroporation increased as concentration of DNA increased from 5 to 5Oμg/6μl, and reached peak at third day after transfection.
6) MnSOD gene-transfection by in vivo electroporation increased the in vivo effects of radiotherapy and hyperthermia. MnSOD gene was transfected by in vivo electroporation for FSa-II tumor in C3H/He mice. Tumors were irradiated at graded doses or heated at 44℃ for 60 min at third day after gene-transfection. The effects of treatments were analyzed by tumor growth-50 method and tumor control dose-50 method.
This study indicated that MnSOD overexpression increased thermosensitivity of cells in vitro, and substantially reduces the tumorigenicity, resulting in a significant increase in tumor growth time in vivo. Reduced tumorigenicity by MnSOD overexpression also resulted in a significant increase in tumor control rate by radiotherapy in vivo.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Masahiro Kuroda: "A New Experimental System for Irradiating Tumors in Mice Using a Linear Accelerator Under Specific Pathogen-free Conditions"Acta Med Okayama. 53. 111-118 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Osamu Honda: "Assessment of Secondary Necrosis of Jurkat Cells Using a New Microscopic System and Double Staining Method with Annexin V and Propidium Iodide"Int J Oncology. 16. 283-288 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masahiro Kuroda,: "Overexpression of Manganese Superoxiade Dismutase Gene Suppresses Spontaneous Apoptosis without a Resultant Alteration in In Vivo Growth of the Mouse Fibrosarcoma.FSa-II"Anticancer Research. 20. 7-10 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshitomo Ando: "The Effect of Calcium on Fas-Mediated Apoptosis and Secondary Necrosis of Jurkat Cells"Int J Mol Med. 7. 243-247 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masahiro Kuroda: "Application of a New Fluorescent Microscopic System for Research in Radiation Biology"J Jpn Soc Ther Radiol Oncol. (In press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masahiro Kuroda: "Basis of Gene-thermotherapy with Manganese Superoxide Dismutase Gene"Jpn J Hypertherm Oncol. (In press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masahiro Kuroda et al.: "A New Experimental System for Irradiating Tumors in Mice Using a Linear Accelerator Under Specific Pathogen-free Conditions"Acta Med Okayama. 53. 111-118 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Osamu Honda et al.: "Assessment of Secondary Necrosis of Jurkat Cells Using a New Microscopic System and Double Staining Method with Annexin V and Propidium Iodide"Int J Oncology. 16. 283-288 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masahiro Kuroda et al.: "Overexpression of Manganese Superoxiade Dismutase Gene Suppresses Spontaneous Apoptosis without a Resultant Alteration in In Vivo Growth of the Mouse Fibrosarcoma, FSa-II"Anticancer Research. 20. 7-10 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshitomo Ando et al.: "The Effect of Calcium on Fas-Mediated Apoptosis and Secondary Necrosis of Jurkat Cells"Int J Mol Med. 7. 243-247 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masahiro Kuroda et al.: "Application of a New Fluorescent Microscopic System for Research in Radiation Biology"J Jpn Soc Ther Radiol Oncol. (In press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masahiro Kuroda et al.: "Basis of Gene-thermotherapy with Manganese Superoxide Dismutase Gene"Jpn J Hypertherm Oncol.. (In press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masahiro Kuroda et al.: "Overexpression of manganese superoxide dismutase gene suppresses spontaneous apoptosis without a resultant alteration in in vivo growth of the mouse fibrosarcoma. FSa-II"Anticancer Res. 7-10 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y Ando,Masahiro Kuroda et al.: "The Effect of Calcium on Fas-Mediated Apoptosis and Secondary Necrosis of Jurkat Cells"Int J Mol Med. 243-247 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Masahiro Kuroda et al.: "Application of a New Fluorescent Microscopic System for Research in Radiation Biology"J Jpn Soc Ther Radiol Oncol. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Masahiro Kuroda et al.: "Basis of Gene-thermotherapy with Manganese Superoxide Dismutase Gene"Jpn J Hypertherm Oncol. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Masahiro Kuroda et al.: "A new experimental system for irradiating tumors in mice using a linear accelerator under specific pathogen-free conditions"Acta Med Okayama. 53,3. 111-118 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Osamu Honda, Masahiro Kuroda et al.: "Assessment of Secondary Necrosis of Jurkat Cells Using a New Microscopic System and Double Staining Method with Annexin V and Propidium Iodide"Int J Oncology. 16. 283-288 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masahiro Kuroda et al.: "Overexpression of manganese superoxide dismutase gene suppresses spontaneous apoptosis without a resultant alteration in vivo growth of the mouse fibrosarcoma, FSa-II"Anticancer Res. (In press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masahiro Kuroda: "A new experimental system for irradiating tumors in mice using a linear accelerator under specific pathogen-free conditions" Acta Med Okayama. 53,2 (in press). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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