| Project/Area Number |
10470223
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Fukui Medical University (1999)
Gunma University (1998) |
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Principal Investigator |
MIYAMOTO Kaoru Fukui Medical University, Department of Biochemistry, Professor, 医学部, 教授 (30125877)
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| Co-Investigator(Kenkyū-buntansha) |
INAZU Tetsuya Fukui Medical University, Department of Biochemistry, Assistant Professor, 医学部, 助手 (00242587)
YAMADA Kazuya Fukui Medical University, Department of Biochemistry, Associate Professor, 医学部, 助教授 (20263238)
峯岸 敬 群馬大学, 医学部, 講師 (00209842)
保坂 公平 群馬大学, 医学部, 教授 (70108992)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | transcription factor / gonadotropin / inducible gene / gene expression / 卵巣顆粒膜細胞 / 卵胞発育 |
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| Research Abstract |
In this study, by using the subtraction cloning method, we have cloned and characterized novel zinc-finger containing genes, that were rapidly induced by PMSG in the rat ovary. Structural analysis of the genes revealed that they are members of C2H2 zinc-finger class of transcription factors. The zinc-finger repeat is first found in Xenopus transcription factor TFIIIA and subsequently found in a large number of eukaryotic regulatory proteins. Therefore we named them Gonadotropin Inducible Transcription Factors (GIOTs). GIOTs constitute a gene family of two closely related genes, which are designated GIOT-1 and GIOT-2. GIOT-1 is expressed solely in steroidogenic tissues and pituitary, whereas GIOT-2 distributes widely in rat tissues. GIOT-1 is strongly induced by gonadotropins in the rat ovary and can suppress transcription of genes having a heterologous promoter construct. Therefore GIOT is actually active in the ovary to suppress gene expression.
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