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Research on the role of a new hormone-inducible transcription factor GIOT for the ovarian function.

Research Project

Project/Area Number 10470223
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Endocrinology
Research InstitutionFukui Medical University (1999)
Gunma University (1998)

Principal Investigator

MIYAMOTO Kaoru  Fukui Medical University, Department of Biochemistry, Professor, 医学部, 教授 (30125877)

Co-Investigator(Kenkyū-buntansha) INAZU Tetsuya  Fukui Medical University, Department of Biochemistry, Assistant Professor, 医学部, 助手 (00242587)
YAMADA Kazuya  Fukui Medical University, Department of Biochemistry, Associate Professor, 医学部, 助教授 (20263238)
峯岸 敬  群馬大学, 医学部, 講師 (00209842)
保坂 公平  群馬大学, 医学部, 教授 (70108992)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordstranscription factor / gonadotropin / inducible gene / gene expression / 卵巣顆粒膜細胞 / 卵胞発育
Research Abstract

In this study, by using the subtraction cloning method, we have cloned and characterized novel zinc-finger containing genes, that were rapidly induced by PMSG in the rat ovary. Structural analysis of the genes revealed that they are members of C2H2 zinc-finger class of transcription factors. The zinc-finger repeat is first found in Xenopus transcription factor TFIIIA and subsequently found in a large number of eukaryotic regulatory proteins. Therefore we named them Gonadotropin Inducible Transcription Factors (GIOTs). GIOTs constitute a gene family of two closely related genes, which are designated GIOT-1 and GIOT-2. GIOT-1 is expressed solely in steroidogenic tissues and pituitary, whereas GIOT-2 distributes widely in rat tissues. GIOT-1 is strongly induced by gonadotropins in the rat ovary and can suppress transcription of genes having a heterologous promoter construct. Therefore GIOT is actually active in the ovary to suppress gene expression.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] Mizutani T.: "Molecular cloning and functional expression of chicken luteinizing hormone receptor"Biochem. Biophs. Acta.. 1397. 1-8 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kishi H.: "The effect of activin and FSH on the differentiation of rat granulosa cells"FEBS Letters. 422. 274-278 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe M.: "Glutamine is involved in the dependency of brain neuron survival on cell plating density in culture"NeuroReport. 9. 2353-2357 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Minegishi T.: "Control of FSH receptor mRNA expression in rat granulosa cells by 3',5'-cyclic adenosine monophosphate, activin, and follistatin"Mol Cell Endocrinol.. 149(1-2). 71-77 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tano M.: "The effect of follicle-stimulating hormone(FSH) on the expression of FSH receptor in cultured rat granulosa cells"Life Sci.. 64(12). 1063-1069 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hirakawa T.: "Effect of prolactin on the expression of LH-receptors during cell differentiation in cultured rat granulosa cells"Endocrinology. 140. 3444-3451 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hosaka K.: "Localization and developmental changes of mRNA for CDP-diacylglycerol synthase in rat central nervous system"The Kitakanto Med. J.. 49. 235-241 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kameda T.: "Regulation of cAMP responsive element binding modulator isoforms in cultured rat ovarian granulosa cells"Biochim. Biophys. Acta.. 1445. 31-38 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsuchiya M.: "Control of the expression of LH-receptor by local factors in rat granulosa cells"Arch. Biochem. Biophys.. 367. 185-192 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hirakawa T.: "A role of IGF-I in LH receptor expression in granulosa cells"Endocrinology. 140. 4965-4971 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Inazu T.: "Cloning and expression of pleckstrin 2, a novel member of the pleckstrin family"Biochem. Biophys. Res. Commun.. 265(1). 87-93 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Minegishi T.: "Retinoic Acid (RA) Represses Follicle Stimulating Hormone (FSH)-Induced Luteinizing Hormone (LH) Receptor in Rat Granulosa Cells"Arch. Biochem. Biophys.. 373(3). 203-210 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mizutani T., Minegishi T., Nonobe Y., Abe Y., Hasegawa Y., Wakabayashi K., Kamiyoshi M., Miyamoto K.: "Molecular cloning and functional expression of chicken luteinizing hormone receptor."Biochim. Biophs. Acta. 1397. 1-8 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kishi H., Minegishi T., Tano M., Kameda T., Ibuki Y., Miyamoto K.: "The effect of activin and FSH on the differentiation of rat granulosa cells."FEBS Letters. 422. 274-278 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe M., Ohe Y., Katakai K., Kobayashi L., Miyamoto K., Ishikawa K.: "Glutamine is involved in the dependency of brain neuron survival on cell plating density in culture."NeuroReport. 9. 2353-2357 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Minegishi T., Kishi H., Tano M., Kameda T., Hirakawa T., Miyamoto K.: "Control of FSH receptor mRNA expression in rat granulosa cells by 3',5'-cyclic adenosine monophosphate, activin, and follistatin."Mol. Cell. Endocrinol.. 149(1-2). 71-77 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tano M., Minegishi T., Kishi H., Kameda T., Abe Y., Miyamoto K.: "The effect of follicle-stimulating hormone (FSH) on the expression of FSH receptor in cultured rat granulosa cells."Life Sci.. 64(12). 1063-1069 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hirakawa T., Minegishi T., Tano M., Kameda T., Kishi H., Ibuki Y., Mizutani T., Miyamoto K.: "Effect of prolactin on the expression of LH-receptors during cell differentiation in cultured rat granulosa cells."Endocrinology. 140. 3444-3451 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hosaka K., Imai H., Fukusato T., Mizutani T., Miyamoto K.: "Localization and developmental changes of mRNA for CDP-diacylglycerol synthase in rat central nervous system."The Kitakanto Med. J.. 49. 235-241 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kameda T., Mizutani T., Minegishi T., Ibuki Y., Miyamoto K.: "Regulation of cAMP responsive element binding modulator isoforms in cultured rat ovarian granulosa cells."Biochim. Biophys. Acta. 1445. 31-38 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsuchiya M., Minegishi T., Kishi H., Tano M., Kameda T., Hirkawa T., Ibuki Y., Mizutani T., Miyamoto K.: "Control of the expression of LH-receptor by local factors in rat granulosa cells."Arch. Biochem. Biophys.. 367. 185-192 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hirakawa T., Minegishi T., Abe K., Kishi H., Ibuki Y., Miyamoto K.: "A role of IGF-I in LH receptor expression in granulosa cells."Endocrinology. 140. 4965-4971 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Inazu T., Yamada K., Miyamoto K.: "Cloning and expression of pleckstrin 2, a novel member of the pleckstrin family."Biochem. Biophys. Res Commun. 265(1). 87-93 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Minegishi T., Hirakawa T., Kishi H., Abe K., Ibuki Y., Miyamoto K.: "Retinoic Acid (RA) Represses Follicle Stimulating Hormone (FSH)-Induced Luteinizing Hormone (LH) Receptor in Rat Granulosa Cells."Arch. Biochem. Biophys.. 373(1). 203-210 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mizutani, T.: "Molecular cloning and functional expression of chicken luteinizing hormone receptor."Biochim. Biophs. Acta.. 1397. 1-8 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kishi, H.: "The effect of activin and FSH on the differentiation of rat granulosa cells."FEBS Letters. 422. 274-278 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Watanabe, M.: "Glutamine is involved in the dependency of brain neuron survival on cell plating density in culture."NeuroReport. 9. 2353-2357 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Minegishi, T.: "Control of FSH receptor mRNA expression in rat granulosa cells by 3',5'-cyclic adenosine monophosphate, activin, and follistatin."Mol Cell Endocrinol.. 149(1-2). 71-77 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tano, M.: "The effect of follicle-stimulating hormone (FSH) on the expression of FSH receptor in cultured rat granulosa cells."Life Sci.. 64(12). 1063-1069 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hirakawa, T.: "Effect of prolactin on the expression of LH-receptors during cell differentiation in cultured rat granulosa cells."Endocrinology. 140. 3444-3451 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hosaka, K.: "Localization and developmental changes of mRNA for CDP-diacylglycerol synthase in rat central nervous system."The Kitakanto Med. J.. 49. 235-241 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kameda, T.: "Regulation of cAMP responsive element binding modulator isoforms in cultured rat ovarian granulosa cells."Biochim. Biophys. Acta.. 1445. 31-38 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tsuchiya, M.: "Control of the expression of LH-receptor by local factors in rat granulosa cells."Arch. Biochem. Biophys.. 367. 185-192 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hirakawa, T.: "A role of IGF-I in LH receptor expression in granulosa cells."Endocrinology. 140. 4965-4971 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Inazu, T.: "Cloning and expression of pleckstrin 2, a novel member of the pleckstrin family."Biochem. Biophys. Res. Commun.. 265(1). 87-93 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Minegishi, T.: "Retinoic Acid (RA) Represses Follicle Stimulating Hormone (FSH)-Induced Luteinizing Hormone (LH) Receptor in Rat Granulosa Cells."Arch. Biochem. Biophys.. 373(1). 203-210 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Mizutani et.al: "Molecular cloning and functional expression of dioken lateinzing biormono receptor" Biochim.Biophs.Acta. 1397. 1-8 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Kishi et.al: "The effect of activin and FSH on the differentiation of rat granulosa cells." FEBS.Letters. 422. 274-278 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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