| Project/Area Number |
10470248
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Keio University |
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Principal Investigator |
AIKAWA Naoki Department of Emergency and Critical Care Medicine, School of Medicine, Keio University, Professor, 医学部, 教授 (40110879)
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| Co-Investigator(Kenkyū-buntansha) |
FUJISHIMA Seitaro Keio University, School of Medicine, Assistant Professor, 医学部, 講師 (00173419)
AOKI Katsunori Keio University, School of Medicine, Associate Professor, 医学部, 助教授 (20124927)
HORI Shingo Keio University, School of Medicine, Associate Professor, 医学部, 助教授 (80129650)
SEKINE Kazuhiko Keio University, School of Medicine, Instructor, 医学部, 助手 (90296715)
KIMURA Hiroyuki Keio University, School of Medicine, Instructor, 医学部, 助手 (00225054)
山崎 元靖 慶應義塾大学, 医学部, 助手 (00296716)
堀口 崇 慶應義塾大学, 医学部, 助手 (70245520)
篠沢 洋太郎 慶應義塾大学, 医学部, 講師 (30129465)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥12,800,000 (Direct Cost: ¥12,800,000)
Fiscal Year 1999: ¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1998: ¥7,000,000 (Direct Cost: ¥7,000,000)
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| Keywords | interleukin-8 / cytokine / body temperature / whole blood culture / hypothermia / hyperthermia / TNFα / IL-6 |
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| Research Abstract |
We initially measured the plasma levels of cytokines, soluble receptors and examined their relationships to the degree of systemic infection and body temperature. In the presence of apparent infection, the plasma IL-6 and IL-8 levels were significantly increased. We found a significant correlation between body temperature and TNF-alpha, IL-6 and IL-8. We then analyzed the hyperthermic patients, whose consciousness was transiently disturbed and who did not show any signs of infection, and found that the plasma levels of IL-6, IL-8 and IL-10 were initially high and then decreased gradually, These data suggested that hyperthermia itself could induce the production of cytokines.
In the second experiment, we examined the effect of environmental temperature on IL-8 production in heparinized whole blood in vitro. Small volume of IL-8 was produced without any stimuli. Interestingly, IL-8 production, in response to endotoxin was rather inhibited when incubated at 42℃, 40℃, but not at 38℃. In combination with above in vivo findings, we speculated that cytokine or cytokine-stimulating factor may be produced by no hematological cells in hyperthermic patients. Additionally, we have made a system for future analysis of cell functions, such as calcium response, cell surface markers, etc.
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