| Project/Area Number |
10470255
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SETO Yasuyuki Department of Surgery, The University of Tokyo, Assistant Professor, 医学部・附属病院・分院, 講師 (00260498)
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| Co-Investigator(Kenkyū-buntansha) |
TSUNO Nelson Department of Surgery, The University of Tokyo, Assistant Professor, 医学部・附属病院, 助手 (50282637)
NAGAWA Hirokazu Department of Surgery, The University of Tokyo, Professor, 医学部・附属病院, 教授 (80228064)
KITAYAMA Joji Department of Surgery, The University of Tokyo, Assistant Professor, 医学部・附属病院, 講師 (20251308)
波多野 賢二 東京大学, 医学部附属病院, 助手
松田 圭二 東京大学, 医学部附属病院, 助手
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥3,100,000 (Direct Cost: ¥3,100,000)
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| Keywords | Chemokine / MIP-1β / Gastric Cancer / Immunohistochemistry / MIPIβ |
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| Research Abstract |
Background. Recently, it has been shown that some precancerous tissues and malignant tumors as well as cultured cancer cell lines are capable of secreting chemokines. Macrophage inflammatory protein-1 β(MIP-β) belongs to the C-C chemokine family that is implicated in a wide range of inflammatory processes. However, how the MIP-β affect tumor growth is unknown.
Method. With the specific mAb to MIP-1β, we examined the expression of MIP-1β in surgically resected gastric carcinomas using immunohistochemistry, and compared the expression pattern in various carcinomas with different histology.
Result. Weak staining of MIP-1β was detected in 34.6% of differentiated carcinomas, but their staining intensity in each cell was less prominent than in normal epithelium. Undifferentiated carcinomas showed contrasted staining pattern between solid type and diffuse type. MIP-β was completely absent in all the undifferentiated carcinoma with solid type growth pattern. In contrast, MIP-1β was strongly expressed in 68% of non-solid type of poorly differentiated carcinoma and 83% of signet-ring cell carcinoma samples. In particular, MIP-1β was strongly stained in carcinoma cells at the front of invasive lesions. In any pathological types, the expression level of MIP-1β did not have a significant correlation with the degree of mononuclear cell infiltration in cancer tissue.
Conclusion. Our results suggest the possibility that MIP-1β production in gastric epithelium may be related to the development or progression of gastric cancer, although it does not seem to play a major role in the recruitment of immune cells in cancer tissue. Especially, MIP-1β may contribute to the invasion step of gastric carcinoma cells with undifferentiated phenotype.
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