| Project/Area Number |
10470264
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Keio University |
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Principal Investigator |
KAWAKAMI Yutaka Keio University, School of Medicine, Professor, 医学部, 教授 (50161287)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Yuriko Keio University, School of Medicine, Instructor, 医学部, 助手 (40255435)
FUJITA Tomonobu Keio University, School of Medicine, Instructor, 医学部, 助手 (20199334)
IKEDA Hideyuki Keio University, School of Medicine, Assistant Professor, 医学部, 専任講師 (40301494)
KITAGAWA Yuko Keio University, School of Medicine, Instructor, 医学部, 助手 (20204878)
KITAJIMA Masaki Keio University, School of Medicine, Professor, 医学部, 教授 (90112672)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 1999: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥10,000,000 (Direct Cost: ¥10,000,000)
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| Keywords | Tumor antigens / SEREX / Antibody / T cells / Pancreatic cancer / Esophageal cancer / Colon cancer / Melanoma / 悪性黒色腫 / 固形癌 / 膀胱癌 |
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| Research Abstract |
To isolate human tumor antigens for immunotherapy of various cancers including esophageal, pancreatic and colon cancers, we applied the SEREX method, expression DNA cloning for the isolation of cDNAs encoding cancer proteins that could induce IgG antibody responses in patients with cancer. Using serum and cDNA library from a melanoma patient, a novel antigen KU-MEL1 was isolated. This antigen was not expressed in most normal tissues tested in the exception of cultured melanocytes, however, it was expressed in most melanoma cells as well as in various adonocarcinoma and squamous cell carcinoma. Using sera and cDNA library from patients with pancreatic cancer, a novel antigen KU-PAN1 was isolated. This antigen was not expressed in most normal tissues in the exception of testis, but expressed in some of the pancreatic, breast and colon cancers. Using sera and cDNA library from patients with esophageal cancer, previously isolated cancer-testis antigen, MAGE-A4, was isolated. MAGE-A4 was shown to be recognized by CD8+ T cells. Using sera and cDNA library from patients with colon cancer, EIF4 and AHNAK nucleoproteins were isolated. IgG antibodies specific for the antigens identified in the present study were detected in some sera from patients with various cancers, but not detected in sera from healthy individuals. Using sera and cDNA library from patients with bladder cancer, the protein homologous to ecalectin was isolated, and IgG antibody against this antigen was frequently detected in sera from patients with esophageal cancers. Therefore, these newly isolated tumor antigens may be useful for development of new diagnostic methods as well as immunotherapies for patients with various cancers.
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