| Project/Area Number |
10470271
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | YAMAGATA UNIVERSITY |
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Principal Investigator |
SHIMAZAKI Yasuhisa Yamagata Univ, Med, Professor, 医学部, 教授 (60116043)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Takao Yamagata Univ, Med, Associate Professor, 医学部, 助教授 (60138922)
INUI Kiyoshige Yamagata Univ, Med, Assistant Professor, 医学部, 助手 (70250941)
箕輪 隆 山形大学, 医学部, 助手 (50292420)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | Gene therapy / Cell transplantation / Aorta / Marfan syndrome / Marfan症候群 / パーテイクルデリバリー法 / パーティクルデリバリー |
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| Research Abstract |
We examined whether gene transfected cell transplanted into the aortic wall can survive in the tissue and change the circumference of aorta in this experiment. Thoracotomy at the 4^<th> intercostal space was carried out in the 15kg 5 adult canines under the general anesthesia. 500000 LacZ cDNA transfected canine renal cells in the 100 microlitter PBS were injected in the descending thoracic aorta. One week after the cell transplantation, canines were sacrificed. 5 excised descending thoracic aorta were fixed with the 10% buffered formalin, and then immersed in the X-Gal solution. Macroscopic findings revealed that X-Gal positive blue stained area was spread in one-third circumstances of aorta. The adventitia of the aorta showed strong reaction to X-Gal, but the media was not stained well with X-Gal. At the portion of needle insertion, not only adventitia but also media, were stained blue with X-Gal well. Then, 5 pieces of each aorta were paraffin embedded, sliced in 5-micrometer thickness and stained by HE staining for microscopic findings. Many gene transfected cells were located at the border zone between media and adventitia. Interstitial space around the transplanted cell were also stained with X-Gal, that mean the possibility of gene therapy for aortic wall by gene engineered cell transplantation. We conclude that gene engineered cell transplantation is safe and highly efficient method of gene transfection to the aorta. The fragile large vessels of Marfan patients may be treated with this new method.
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