| Project/Area Number |
10470295
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
MATSUSHIMA toshio Kyushu University, Graduate School of Medicine, Associated Professor, 大学院・医学系研究科, 助教授 (40165816)
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| Co-Investigator(Kenkyū-buntansha) |
IKEZAKI Kiyonobu Kyushu University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (10145360)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥11,700,000 (Direct Cost: ¥11,700,000)
Fiscal Year 1999: ¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1998: ¥6,500,000 (Direct Cost: ¥6,500,000)
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| Keywords | moyamoya disease / HLA / Genetic abnormality |
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| Research Abstract |
1. Linkage study
In the 19th families affected by moyamoya disease, blood samples was obtained from all members of the family. Microsatellite markers on the 6th chromosome were amplified by using polymerase chain reaction. Sharing of the allele was investigated among affected members, considering the haplotype. The marker, D6S441 had a possible linkage with moyamoya disease. Thus, around the D6S441, responsible gene for moyamoya disease might be located. Another institute analyzed and reported that chromosome 3 was linked to moyamoya disease. According to clinical genetics, moyamoya disease shows multifactorial inheritance. Further analysis might show other region linked to moyamoya disease.
2. Polymorphisms of TGFB1 and TGFBR 2 genes
Recent studies have shown increased production of serum transforming growth factor (TGF)βィイD21ィエD2 and its mRNA level from cultured smooth muscle cells in patients with Moyamoya disease. TGF-β signaling system has been suggested to be associated with the pathogenesis of Moyamoya disease. We analyzed the polymorphisms of TGFB1 and TGFBR 2 genes in 61 Moyamoya patients. Our study has shown that there were no associations between Moyamoya disease and polymorphisms of TGFB1/TGFBR 2 genes. Thus, it is likely that increases of TGFβ1 in serum and vascular smooth muscle cells were not primary but secondary events in Moyamoya disease.
3. Renin-angiotensin system
The plasma levels of renin, angiotensin I, and angiotensin II were investigated in 48 Moyamoya patients. The level of angiotensin I was markedly elevated in the patients. It was not clear whether this increase was primary or secondary. Further investigation is needed to clarify the fact.
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