| Project/Area Number |
10470304
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Gifu University |
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Principal Investigator |
SHIMIZU Katsuji GIFU UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF ORTHOPAEDIC SURGERY, PROFESSOR, 医学部, 教授 (90170969)
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| Co-Investigator(Kenkyū-buntansha) |
ITOH Yoshiki GIFU UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF ORTHOPAEDIC SURGERY, RESEARCH ASSOCIATE, 医学部, 助手 (10313884)
SAKAGUCHI Yasumichi GIFU UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF ORTHOPAEDIC SURGERY, RESEARCH ASSOCIATE, 医学部附属病院, 助手 (60301214)
HOSOE Hideo GIFU UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF ORTHOPAEDIC SURGERY, ASSISTANT PROFESSOR, 医学部附属病院, 講師 (60219186)
KYUUKI Kouhei JAPAN BIO-RESEARCH CENTER, DIVISION OF PHARMACOLOGY, DIRECTOR, 部長
西本 博文 岐阜大学, 医学部附属病院, 医員
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥10,700,000 (Direct Cost: ¥10,700,000)
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| Keywords | CHEMONUCLEOLYSIS / CALPAIN / CEREBROSPINAL FLUID / 脳脊髄液 cerebrospinal fluid / カルパイン / カルパスタチン / 化学的髄核融解 |
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| Research Abstract |
ON THE CHEMONUCLEOLYSIS WITH CHYMOPAPAIN, COMPLICATIONS SUCH AS ANAPHYLACTIC REACTION AND PARALYSIS HAVE BEEN REPORTED. TO MEKE CHEMONUCLEOLYSIS A SAFER TREATMENT, OTHER SAFE AND POTENT ENZYMES HAVE BEEN NEEDED. AUTOGENOUS μCALPAIN AS PURIFIED FROM THE PATIENT'S OWN RED BLOOD CELLS MIGHT PRIVENT ANAPHYLACTIC REACTION. CALPAIN HAS BEEN SHOWN TO THE DEGRADATIVE ACTIVITY ON PROTEOGLYCAN OF HE INTERVERTEBRAL DISCS OF RABBITS.
IN THE PRESENT STUDY, WE FOUND CEREBROSPINAL FLUID INHIBIT ENZYME REACTION OF CALPAIN IN ITS NORMAL CONCENTRAION. THE MOLECULAR WEIGHT OF CALPASTATIN, SPECIFIC ENDOGENOUS INHIBITOR OF CALPAIN, IS APPROXIMATELY 80-kDa. THE MOLECULAR WEIGHT OF THE SUBSTANCE WHICH INHIBIT CALPAIN ENZYME ACTIVITY IN CEREBROSPINAL FLUID MIGHT BE LESS THAN 10 -kDa. CEREBROSPINAL FLUID WERE SUBJECTED TO SDS-PAGE. IN THE IMMUNOBLOTTING WITH ANTICALPASTATIN ANTIBODY POSITIVE STAINING WAS NOT OBTAINED. THE SUBSTANCE WHICH INHIBIT CALPAIN IN CEREBROSPINAL FLUID MIGHT BE DIFFERENT FROM CALPASTATIN.
THESE DATA SUGGEST IN CASE OF ACCIDENTAL INTRATHECAL INJECTION OF μCALPAIN FOR CHEMONUCLEOLYSIS, ITS ENZYME ACTIVITY WILL BE LOST BY CEREBROSPINAL FLUID. IT IS POSSIBLE THAT AUTOGENOUS μCALPAIN PREVENT FROM ONE OF MAJOR COMPLICATIONS SUCH AS PARALYSIS. WE SUGGEST IT MAKES CHEMONUCLEOLYSIS WITH AUTOGENOUS μCALPAIN A SAFER TREATMENT OF HENIATED INTERVERTEBRAL DISCS.
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