| Project/Area Number |
10470308
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | YOKOHAMA CITY UNIVERSITY |
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Principal Investigator |
KOSHINO Tomihisa SCHOOL OF MEDISINE DEPARTMENT OF ORTHOPAEDIC SURGERY PROFESSOR, 医学部, 教授 (90046029)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAGI Toshitaka SCHOOL OF MEDISINE DEPARITMENT OF ORTHOPAEDIC SURGERY INSTRUCTOR, 附属病院, 講師 (30254171)
SAITO Tomoyuki SCHOOL OF MEDISINE DEPARTMENT OF ORTHOPAEDIC SURGERY ASSISTANT PROFESSOR, 医学部, 助教授 (30170517)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥11,400,000 (Direct Cost: ¥11,400,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1999: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1998: ¥5,300,000 (Direct Cost: ¥5,300,000)
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| Keywords | osteoarthiris of the knee / degenerated cartilage / regenerated cartilage / high tibial osteotomy / growth factors / インスリン様成長因子 |
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| Research Abstract |
High tibial osteotomy (HTO) was performed in osteoarthritic knees. Regeneration of the articular cartilage after HTO was observed and classified. Expression and localization of basic Fibroblast Growth Factor (bFGF) was studied in 46 knees of 39 medial compartmental osteoarthritis patients who underwent the removal of the internal fixation at an average of two years after initial high tibial valgus osteotomy (HTO). The mean age of the donors at HTO were 71 years old. The mean interval between HTO and the removal of the internal fixation was 17 months. Biopsy specimens were taken from the medial femoral condyle which were embedded in paraffin sections. The specimens were stained with toluidine blue and observed under a light microscopy-Quantitative evaluation for bFGF in joint fluid from osteoarthiric patients taken at the removal of the internal fixation after HTO, was performed with ELISA technique. We investigated the relationship between the amount of bFGF and histological findings with the regenerated articular tissue. Higher amount of bFGF was observed in severe osteoarthritic patients. Low amount of bFGF was observed in patients with well-regenerated fibrocartilageous tissue. Hereafter we intend to evaluate correlation with cartilage regeneration and growth factors such as Carboxy-terminal type II peptide (pCOL-II-C) in more osteoarthiric patients and rabbits models.
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