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Evaluation of role of central nervous CaィイD1[2+]ィエD1 channel subtype in pain regulation system and its significance

Research Project

Project/Area Number 10470326
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionTokyo Medical University

Principal Investigator

ISSIKI Atsusi  Tokyo Medical University, Anesthesiology, Professor, 医学部, 教授 (60074796)

Co-Investigator(Kenkyū-buntansha) OGIWARA Yukihiko  Tokyo Medical University, Anesthesiology, Assis Prof, 医学部, 講師 (40224141)
OUMI Akifumi  Tokyo Medical University, Anesthesiology, Assis Prof, 医学部, 講師 (70246221)
WATANABE Yasuo  Tokyo Medical University, Pharmacology, Assos Prof, 医学部, 助教授 (70183720)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1999: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsCaィイD12+ィエD1 / L-type CaィイD12+ィエD1 channel / T-type CaィイD12+ィエD1 channel / pain / cyclooxygenase II / spinal neuron / GABAa receptor / GABAb receptor / flinching / 電位依存性Ca^<2+>チャンネル / 疼痛制御機構 / 二次性疼痛 / c-fos / Bcl-2 / L型 / T型 / 腰髄
Research Abstract

In the neuronal pathway of the spinal cord, L- and T-type CaィイD12+ィエD1 channels, cyclooxygenase(COX) isozyme and GABA receptor subtype play important roles in pain regulation. In this experiment, we compared the effects of intralthecal injections of above specific inhibitors on secondary nociception induced by formalin using male Wistar rats(about 300g). As specific inhibitors, nimodipine(L-type), flunarizine(T-type), NS398(COX II), muscimol(GABAa) and baclofen(GABAb) were used.All agents were abministered intranthecally 10 min prior to formalin injection.Flinching was quantified in five minute intervals for 60min. These inhibitors significantly blocked formalin-induced pain. Particularly, intrathecal injection of flunarizine/baclofen showed the strong inhibition. Flunarizine also suppressed the appearance of c-fos induced by formalin. However, the larger dosage of NS398, which partially suppressed COX I, did not show any inhibition on formalin-induced pain. This NS398 dosage enhanced the nociceptive response. In conclusion, the prostanoid synthetic pathway activated by cytosolic CaィイD12+ィエD1 via T-type channel in the spinal cord appears to strongly influence nociceptive response, and the physiological balance between COX I and II activities may be associated with the regulation of prostanoid synthesis and GABAb receptor.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] 太田隆史 他: "細胞内Ca調節薬のフォルマリン誘発2次性疼痛反応に及ぼす影響"ペインクリニック. 20. 636-642 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Okita et al: "Presynaptic L-type Ca^<2+> channel on dopanine excessive release from rat caudate putamen"Physiol & Behav. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] TAYA et al: "Appearance of high sensitive cells to L-type Caantagonist in cultured cerebellar gramle cells"Japan J Nearopsychopharmaco 1. 19. 133-139 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohta, Sogabe, Issiki, Kitamura, Watanabe,: "Effects of cytosolic Ca ion modulators on the secondary pain induced by formalin."Pain Clinic. 20. 636-642 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Okita, Watanabe, Taya, Utsumi, Hayashi,: "Presynaptic L-type CaィイD12+ィエD1 channel on dopamine excessive release from rat caudate putamen"Physiol & Behav. in press. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Taya, Watanabe,: "Appearance of high sensitive cells to L-type Ca antagonist in calfared cerebellar gramile cells"Japan J, Neuropsychopha-macol. 19. 133-139 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 太田隆史 他: "細胞内Ca調節薬のフォルマリン誘発2次性疼痛反応に及ぼす影響"ペインクリニック. 20. 636-642 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Okita et al: "Presynapticl-typeCa^<2+>channel on dopamine excessive from rat coudate pitome."Physiol & Behan. in press. (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Taya et al: "Appearence of high sensitive cells to L-type Caantagonist in Hared cerebellar groaule cells"Japan J Neuropyschophavmeal. 19. 133-139 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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