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Dynamics and Biological Significance of Vascular Growth Factors in Urogenital Cancers.

Research Project

Project/Area Number 10470330
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionAkita University

Principal Investigator

KATO Tetsuro  Akita University School of Medicine, Professor, 医学部, 教授 (40004642)

Co-Investigator(Kenkyū-buntansha) SATO Kazunari  Akita University School of Medicine, Lecturer, 医学部, 講師 (90270842)
SATOH Shigeru  Akita University School of Medicine, Lecturer, 医学部, 講師 (80187195)
OGAWA Osamu  Kyoto University School of Medicine, Professor, 医学部, 教授 (90260611)
MISHINA Mutsuki  Akita University School of Medicine, Research Assoc., 医学部, 助手 (30301063)
佐々木 隆聖  秋田大学, 医学部, 助手 (90292375)
土屋 順彦 (土谷 順彦)  秋田大学, 医学部, 助手 (70282176)
赤尾 利弥  秋田大学, 医学部, 助手 (40301064)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2000: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1998: ¥6,400,000 (Direct Cost: ¥6,400,000)
Keywordsangiogenic factor / urogenital cancer / VEGF / bFGF / ELISA / angiogenesis / 腎細胞癌 / VEGF受容体 / 遺伝子発現 / 定量的PCR
Research Abstract

The relationship between tumor angiogenesis and angiogenic factors was evaluated in urogenital cancers. VEGF gene was overexpressed in more than a half of renal cell carcinoma (RCC). The expression level of VEGF genes was significantly correlated with the intratumoral microvessel density. An immunohistologic analysis revealed that cytoplasm of RCC appeared to be positive for the VEGF staining. VEGF gene was also overexpressed in 40% of BCas. At protein level, VEGF was apparently positive in bladder carcinoma cells. VEGF-receptor genes were expressed in concordance with the VEGF gene expression level in both tumors. Thus, angiogenesis in RCC and bladder cancer is suggested to be facilitated by a paracrine mechanism with VEGF and its receptors.
We studied the expression of VEGF-relating genes (VEGF-A, B, C, and D) in RCCs and found that VEGF-A and the receptors, VEGFR-1 and VEGFR-2, play crucial roles in the angiogenesis of RCC while VEGF-B and VEGF-C do little.
Based on the above results, we compared serum VEGF levels between cancer patients and healthy control subjects. The mean serum VEGF level was higher in the RCC patient group than in the healthy control group. Postoperative serum VEGF level apparently decreased as compared with the preoperative level. Serum VEGF level was significantly correlated with tumor volume, metastasis, and stage. The mean serum VEGF level was also higher in the bladder cancer patient group than in the healthy control group. Thus we suggest that RCC and bladder cancer release VEGF into the blood circulation and serum VEGF level would be a marker of malignant potential of these tumors.
Taken together, these results would be useful as basic data for the development of antiangiogenic-factor antibody facilitating diagnosis and therapy of RCC and bladder cancer.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Kazunari Sato et al.: "Increased serum levels of vascular endothelial growth factor in patients with renal cell carcinoma."Jpn J Cancer Res. 90. 874-879 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kazunari Sato et al.: "Expression of vascular endothelial growth factor gene and its receptor(flt-1) gene in urinary bladder cancer."Tohoku J Exp Med. 185. 173-184 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 佐藤一成: "血管新生からみた腎細胞癌の治療"大塚泌尿器がん懇話会会報. 5. 7 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tsuchiya N et al: "Gene expression of neuropilin-1, an isoform specific receptor for VEGF165, is increased in human renal cell carcinoma."J Urol. 163. 501A (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sato K et al.: "Increased serum levels of vascular endothelial growth factor in patients with renal cell carcinoma."Jpn J Cancer Res. 90. 874-879 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sato K et al.: "Expression of vascular endothelial growth factor gene and its receptor (flt-1) gene in urinary bladder cancer."Tohoku J Exp Med. 185. 173-184 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sato K: "Therapy for renal cell carcinoma oriented to angiogenesis."Proceeding of 5th Otsuka Urologic Oncology Seminar. 5. 7 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tsuchiya N et al.: "Gene expression of neuropilin-1, an isoform specific receptor for VEGF165, is increased in human renal cell carcinoma."J Urol. 163. 501A (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tsuchiya N et al: "Gene expression of neuropilin-1,an isoform specific receptor for VEGF165,is increased in human renal cell carcinoma. "J Urol. 163. 501A (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 佐藤一成: "血管新生からみた腎細胞癌の治療."大塚泌尿器がん懇話会会報. 5. 7 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kazunari Sato, et al.: "Increased serum levels of vascular endothelial growth factor in patients with renal cell carcinoma"Jpn J Cancer Res. 90. 874-879 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Norihiko Tsuchiya, et al.: "Quantitative analysis of VEGF,VEGF-C and VEGFR-2 gene expression in renal cell carcinoma"J Urol. 161. 143A (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sato K, et al.: "Expression of vascular endothelial growth factor gene and its receptor (flt-1) gene in urinary bladder cancer." Tohoku J Exp Med. 185(3). 173-184 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 小倉泰伸、他: "表在性膀胱癌における血管新生因子の発現と腫瘍血管密度に関する免疫組織学的検討" 日本泌尿器科学会雑誌. 80(5). 529-537 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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