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Transcriptional regulation of rhodopsin gene under light dark cycle

Research Project

Project/Area Number 10470360
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

MATSUDA Hidehiko  Hokkaido Univ., Sch. Of Med., Professor, 医学部, 教授 (80000959)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Kazuhiko  Hokkaido Univ., Sch. Of Med., instructor, 医学部, 助手 (90281807)
SAKAI Masaharu  Hokkaido Univ., School Of Medicine, associate professor, 医学部, 助教授 (50162269)
IMAKI Junko  Department of Anatomy, Nippon Medical College, Associate Professor, 医学部, 講師 (20223323)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥12,300,000 (Direct Cost: ¥12,300,000)
Fiscal Year 1999: ¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1998: ¥6,800,000 (Direct Cost: ¥6,800,000)
Keywordsc-fos / rhodopsin / transcription / light / promotor / 明暗環境 / mGluR6 / CREB / FOS蛋白 / 転写制御 / 明暗変化 / 日内変動
Research Abstract

The signal pathway transcriptional regulation of rhodopsin gene under light dark cycle was investigated. The oligo nucleotide of the promoter cite of the rhodopsin ran slower in the gel retardation assay when incubated with the nuclear extract of the retina, suggesting that there are something in the nucleus of the retina that bind the site.
In addition, the oligo nucleotide ran even slower when incubated with anti-c-Fos antibody and the nuclear extract of the retina, suggesting that c-Fos protein in the retina bind the promoter cite of the rhodopsin gene. We are now eramining the role of the c-fos gene in the transcriptional regulation of the rhodopsin in vivo by using c-fos knockout mice.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Kitaichi N.et al.: "Prominent increase of macrophage migration inhibitory factor in the sera of patients with uveitis"Invest Ophthamol Vis Sci. 40. 247-250 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kotake S.et al.: "Central nervous system symptoms in patients with Behcet disease receiving cyclosprine therapy"Opthalmology. 106. 586-589 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matuda A.et al.: "Identification and immunohistochemical localization of annexin 2 inrat cornea"Curr Eye Res. 19. 368-375 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Onodera H.et al.: "Differential expression of c-fos mRNA in rat neocortex by instuhybridization"Life Sci.. 64. 1127-1135 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tokita R.et al.: "Prolaction secretion in response to prolactin-releasing peptide and the expression of the prolactin-releasing paptide gene in the medulla oblongata are eatrogen dependent in rats"Neurosci.Lett.. 276. 103-106 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamada K.et al.: "Hyper responsiveness to palatable and aversive taste stimuli in genetically obese (bonbesin recepter subtype-3-dificiet) mice"Physiol Behav.. 66. 863-867 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kitaicbi, N et al.: "Prominent increase of macrophage migration inhibitory factor in the sera of patients with uveitis."Invest Ophthahnel Vis Sci.. 40. 247-250 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kotake. S et al.: "Central nervous system symptoms in patients with Behcet disease receiving cyclosprine therapy."Opthalmology. 106. 586-589 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matuda, A et al.: "Identification and immunohistochemical localization of annexin 2inrat cornea."Curr Eye Res. 19. 368-375 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Onodera, H et al.: "Differential expression of c-fos mRNA in rat neocortex by instuhybridization."Life Sci.. 64. 1127-1135 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tokita, R et al.: "Prolaction secretion in response to prolactin-releasing peptide and the expressin of the prolactin-releasing paptide gene in the medulla oblongata are eatrogen dependent in rats."Neurosei. Lett.. 276. 103-106 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamada, K et al.: "Hyper responsiveness to platable and aversive taste stimuli in genctically obese (bonbesin recepter subtype-3-dificient) mice."Physicl Behav.. 66. 863-867 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kitaichi, N., et al.: "Prominent increase of macrophage migration inhibitory factor in the sera of patients with uveitis"Invest Ophthalmol Vis Sci. 40. 247-250 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kotake, S., et al.: "Central nervous system symptoms in patients with Bechet disease receiving cyclosprine therapy"Opthalmology. 106. 586-589 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matuda, A., et al.: "Identification and immunohistochemical localization of annexin 2 in rat cornea"Curr Eye Res. 19. 368-375 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Onodera, H., et al.: "Differential expression of c-fos mRNA in rat neocortex by instuhybridization"Life Sci.. 64. 1127-1135 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tokita, R., et al.: "Prolaction secretion in response to prolactin-releasing peptide and the expression of the prolactin-releasing peptide gene in the medulla oblongata are eatrogen dependent in rats"Neurosci. Lett.. 276. 103-106 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yamada, K., et al.: "Hyper responsiveness to palatable and aversive taste stimuli in genetically obese (bonbesin recepter subtype-3-dificient) mice"Physiol Behav.. 66. 863-867 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshida Y.et al: "CREB-induced transcriptional activation depends on m GluR6 in rod bipolar cell" Mol.Brain Res.52:2. 241-247 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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