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Immunological and Molecular Biological Research on Local Defense System of the Eye

Research Project

Project/Area Number 10470368
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

MOCHIZUKI Manabu  Department of Ophthalmology & Visual Science, Tokyo Medical and Dental University Graduate School, 大学院・医歯学総合研究科, 教授 (10010464)

Co-Investigator(Kenkyū-buntansha) AKIYAMA Takashi  Department of Ophthalmology & Visual Science, Tokyo Medical and Dental University Graduate School, 医学部, 助手 (50282736)
TANAKA Sumiyoshi  Department of Ophthalmology & Visual Science, Tokyo Medical and Dental University Graduate School, 大学院・医歯学総合研究科, 助手
SEKO Yuko  Department of Ophthalmology & Visual Science, Tokyo Medical and Dental University Graduate School, 大学院・医歯学総合研究科, 助手 (60301157)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥10,000,000 (Direct Cost: ¥10,000,000)
Keywordsuveitis / apoptosis / soluble Fas ligand / soluble Fas / cytokines / aqueous humor / T cell clone / human / 局所防禦機構 / HTLV-I / FPLCカラムクロマトグラフィー / 免疫抑制
Research Abstract

The research was aimed at investigating the local defense system of the eye. The aqueous humor (AH) obtained from the anterior chamber of patients with age-related cataract during cataract surgery was found to suppress the cytokine production by T cell clones established from ocular infiltrating cells of patients with uveitis. Four molecules having the suppressive activities were identified from the AH by FPLC column chromatography. One of the four molecules had similar molecular weight as soluble Fas ligand (sFasL). A significant amount of sFasL was detected in the AH of age-related cataract patients, indicating the presence of sFasL in the AH of human eye at normal condition. In experimental model of uveitis, a significant correlation was found between the uveitis activity and the number of apoptotic cells or FasL-positive cells. These results suggest that sFasL and other immunosuppressive molecules in AH have a significant role to regulate intraocular inflammation. Further studies to examine if sFasL induces suppression of cytokine production or apoptosis of T cell clones established ocular infiltrating cells of uveitis patients are now ongoing at our laboratory.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Ayako Ono, et al.: "Provirus load in patients with human T-cell leulemia virus type 1 uveitis correlates with precedent Graves'disease and disease activites"Japanese Journal of Cancer Research. 89. 608-614 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mami Sakaguchi, et al.: "Cytokine production by T cells infiltrating in the eye of uveitis patients"Japanese Journal of Ophthalmology. 42. 262-268 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Naoko Seki, et al.: "Polymorphism of the 5'-flanking region of the tumor necrosis factor-α gene Susceptibility to human T-cell lymphotropic virus type I uveitis"Journal of Infectious Diseases. 180. 880-883 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 杉田直: "前房水の免疫抑制作用"日本眼科紀要. 51(印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sunao Sugita, et al.: "Soluble Fas ligand and soluble Fas in the ocular fluid ofpatients with uveitis"British Journal of Ophthalmology. 84(印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Jun Sueda, et al.: "Kinetics of apoptotic cells in experimental autoimmune uveoretintis"Investigative Ophthalmology & Visual Science. 41(印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ayako Ono, et al.: "Provirus load in patients with human T-cell leukemia virus type 1 uveitis correlates with precedent Graves' disease and disease activities."Japanese Journal of Cancer Research. 89. 608-614 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mami Sakaguchi, et al.: "Cytokine production by T cells infiltrating in the eye of uveitis patients."Japanese Journal of Ophthalmology. 42. 262-268 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Naoko Seki, et al.: "Polymorphism of the 5'-flanking region of the tumor necrosis factor (TNF)-α gene and susceptibility to human T-cell lymphotropic virus type I (HTLV-I) uveitis."Journal of Infectious Diseases. 180. 880-883 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sunao Sugita, et al.: "Immunosuppressive activities of the aqueous humor"Folia Ophthalmologica Japonica. (in press). 51 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sunao Sugita, et al.: "Soluble Fas ligand and soluble Fas in the ocular fluid of patients with uveitis."British Jouunal of Ophthalmology. (in press). 84 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Jun Sueda, et al.: "Kinetics of apoptotic cells in experimental autoimmune uveoretinitis."Investigative Ophthalmology & Visual Science. (in press). 41 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Naoko Seki, et al.: "Polymorphism of the 5'-flanking region of the tumor necrosis factor-α gene Susceptibility to human T-cell lymphotropic virus type I uveitis"Journal of Infectious Diseases. 180. 880-883 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ayako Ono 他: "Provirus load in patients with human T-cell leukemia virus type 1 uveitis correlates with precedent Graves disease and disease activities" Japanese Journal of Cancer Research. 89巻6号. 608-614 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mami Sakaguchi 他: "Cytokine production by T cells infiltrating in the eye of uveitis patiente" Japanese Journal of Ophthalmology. 42巻10月号. 262-268 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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