Study on Mechanisms of Angiogenesis Induced by Blood Flow using in vivo Experimental Model
| Project/Area Number |
10470373
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | THE UNIVERSITY OF TOKYO |
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Principal Investigator |
SATO Hirotaka DEPARTMENT OF PLASTIC SURGERY, FACULTY OF MEDICINE, UNIVERSITY OF TOKYO, ASSISTANT PROFESSOR, 医学部・附属病院, 講師 (20222581)
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| Co-Investigator(Kenkyū-buntansha) |
ICHIOKA Shigeru DEPARTMENT OF PLASTIC SURGERY, FACULTY OF MEDICINE, UNIVERSITY OF TOKYO, STAFF SURGEON, 医学部・附属病院, 医員 (60306272)
ANDO Joji DEPARTMENT OF BIOMEDICAL ENGINEERING, GRADUATE SCHOOL OF MEDICINE, UNIVERSITY OF TOKYO, ASSOCIATE PROFESSOR, 大学院・医学系研究科, 助教授 (20159528)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 1999: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 1998: ¥9,300,000 (Direct Cost: ¥9,300,000)
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| Keywords | Angiogenesis / Shear stress / Microcirculation |
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| Research Abstract |
The microvascular arteriovenous (A-V) shunt was made on the thigh of the male Wistar rats to modify blood flow. Granulation tissues were formed surrounding the A-V shunt wrapped with artifical skin dermis (ASD). A similar control dissection of these vessels was done but no shunt created.
The vascular structure was observed by the following methods ; (1) Histologic examinations (2) Microangiography (3) Three dimentional observation of vascular casts with a stereo zooming microscope. In addition, the total vascular volume was calculated from measuring weight of vascular casts.
In high blood flow group, shear stress calculated from blood flow and vessel diameter was significantly increased. Scanning electron microscopic observation showed many holes in vessel lumen in both artery and vein, suggesting that new vessels sprout from the loop. There was an increase in the volume of the neovascular around the A-V shunt loop day by day. Total volume of new vessels was four times as great as control.
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Report
(3 results)
Research Products
(13 results)
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[Publications] Ichioka, S., Nakatsuka, T., Sato, Y., Shibata, M., Kamiya, A., and Harii, K.: "Amrinone, a selective phosphodiesterase III inhibitor, improves microcirculation and flap survival : A comparative study with prostaglandin E1"J. Surg. Res.. 75. 42 (1998)
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[Publications] Ichioka, S., Shibata, M., Kosaki, K., Sato, Y., Harii, K., and Kamiya, A.: "In vivo measurement of morphometric and hemodynamic changes in the microcirculation during angiogenesis under chronic a1-adrenergic blocker treatment"Microvasc. Res.. 55. 165 (1998)
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[Publications] Ichioka, S., Minegishi, M., Iwasaka, M., Shibata, M., Nakatsuka, T., Harii, K., Kamiya, A., and Ueno, S.: "High-intensity static magnetic fields modulate skin microcirculation and temperature in vivo."Bioelectromagnetics. (in press).
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