| Project/Area Number |
10470374
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Plastic surgery
|
|---|
| Research Institution | Tokyo Medical and Dental University (1999)
Kagawa Medical School (1998) |
|---|
Principal Investigator |
HATA Yuiro Tokyo Medical and Dental University, Graduate School Division of Maxillofacial/Neck Reconstruction Head and Neck Reconstruction Plastic and Reconstruction Surgery, PROFESSOR, 大学院・医歯学総合研究科, 教授 (90164839)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KOSAKA Hiroaki Kagawa Medical University, Second Department of Physiology, PROFESSOR, 医学部, 教授 (60158897)
|
|---|
| Project Period (FY) |
1998 – 1999
|
| Project Status |
Completed (Fiscal Year 1999)
|
| Budget Amount *help |
¥12,300,000 (Direct Cost: ¥12,300,000)
Fiscal Year 1999: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥9,300,000 (Direct Cost: ¥9,300,000)
|
|---|
| Keywords | MICROCIRCULATION / SKIN FLAP / FREE RADICAL / SUPEROXIDE / REACTIVE OXIGEN / ANGIOTENSIN II / NITRIC OXIDE / HYPERTENSION |
|---|
| Research Abstract |
We investigate the relationship between vessel contractions and free radicals using angiotensin II in the models of a mesentery vessel and a great vessel.
Model of a mesentery vessel : experiment 1 ; Generally the blood flow stops under angiotensin II. In this occasion, incase of adding superoxidedismutase, the stopping time of the blood flow and the reperfusion time became short. experiment 2 ; The mesentery vessels which were extracted and incubated with MCLA gave light i.e. it meant the production of superoxide. experiment 3 ; Superoxidedismutase reduced the contraction of the vessels. These results showed that superoxide would appeared when the vessel contracted and superoxide might influence the contraction.
Model of a great vessel : experiment 1 ; Angiotensin II reduced the increasing of the blood pressure. experiment 2 ; The great vessels which were extracted and incubated with MCLA gave out light. experiment 3 ; Superoxidedismutase reduced the contraction of the great vessels. These results showed that superoxide would appeared when the great vessels contracted and superoxide might influence the blood pressure.
In this study we found a new knowlegement that the vessels produced the superoxide. This suggests that angiotensin II promotes the growth of the smooth muscles inside the vessel under the midiator of superoxide.
|
