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A variety of molecular crosstalks between extracellular matrices and their cell surface receptors in oral carcinomas

Research Project

Project/Area Number 10470379
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionNIIGATA UNIVERSITY

Principal Investigator

SAKU Takashi  NIIGATA UNIVERSITY, Faculty of Dentistry Professor, 歯学部, 教授 (40145264)

Co-Investigator(Kenkyū-buntansha) YONEMOCHI Hiroko  NIIGATA UNIVERSITY, Faculty of Dentistry Assistant, 歯学部, 助手 (60293213)
OHSHIRO Kazufumi  NIIGATA UNIVERSITY, Faculty of Dentistry Assistant, 歯学部, 助手
CHENG Jun  NIIGATA UNIVERSITY, Faculty of Dentistry Associate Professor, 歯学部, 助教授 (40207460)
KIMURA Shin  NIIGATA UNIVERSITY, Faculty of Dentistry Assistant, 歯学部, 助手 (80251825)
ODA Kimimitsu  NIIGATA UNIVERSITY, Faculty of Dentistry Professor, 歯学部, 教授 (10122681)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥12,700,000 (Direct Cost: ¥12,700,000)
Fiscal Year 2000: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1998: ¥8,200,000 (Direct Cost: ¥8,200,000)
Keywordsextracellular matrix / heparan sulfate proteoglycan / fibronectin / integrin / adenoid cystic carcinoma / in-situ hybridization / ACC3 / alternative splicing / 腺様歯原性腫瘍 / 口腔粘膜 / ファイブロクネクチン / 口腔癌 / オリゴ糖鎖
Research Abstract

We have analyzed biosynthesis of extracellular matrix(ECM)molecules by oral carcinoma cells, such as ACC3 established from a human adenoid cystic carcinoma cells, MK-1 and ZK-1 established from oral squamous cell carcinomas. These carcinoma cells were shown to produce basement membrane-associated molecules, especially basement membrane type heparan sulfate proteoglycan, HSPG/perlecan, and fibronectin, FN.
These two ECM molecules were different in molecular weight with cell types. We examined the molecular background for the variations in molecular size among them, by using immunoprecipitation of 35S-methionin labeled cells with several combinations of oligosaccharide lyases. ACC3 cells produced high molecular weight HSPG/FN, which were resulted from larger proteins due to alternative splicing and addition of N-and O-linked oligosaccharide chains, although more investigations are necessary for the alternative splicing mechanism for HSPG core protein.
Similarly, integrins, INTs, receptors … More for ECM molecules, expressed by these cells were different in size. This was mainly due to oligosaccharide additions. Thus, there was a variety of structures of ECM molecules and their receptors among oral carcinoma cells. Since the molecular sizes were parallel with the attachment ability of the cells, it is suggested that the ECM molecular size, which is varied with carcinoma cell types, regulate the biological nature of them.
Such a variety of molecular crosstalks between ECM molecules and their receptors should be also reflected to the tissue architecture of oral carcinomas as well as tissue remodeling processes of granulation tissues. We also immunolocalized these molecules in oral neoplastic and inflammatory lesions. In relation to these immunohistochemical experiments, we proposed a guideline for enzymatic pretreatments for ECM molecules in various tissue types.
These results clearly indicate that the molecular structures of ECM molecules and their receptors vary with cell types and that the molecular variety should be reflected in their clinical courses. However, it is unknown from the present study what regulates such molecular variety and if these varieties are actually functions in human carcinoma tissues in vitro. Less

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] Kimura, S.: "Perlecan (heparan sulfate proteoglycan) gene expression reflected in the characteristic histological architecture of salivary adenoid cystic carcinoma." Virchows Arch. 437(2). 122-128 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Murata, M.: "Enamel protein S and extracellular matrix molecules are co-localized in the pseudocystic stroma space of adenomatoid odontogenic tumor."Journal of Oral Pathology & Medicine. 29(10). 483-490 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kimura, S.: "Basement membrane heparan sulfate proteoglycan (perlecan) synthesized by ACC, adenoid cystic carcinoma cell of human salivary gland origin."Journal of Biochemistry. 25(2). 406-413 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toyoshima, K.: "High-molecular-weight fibronectin synthesized by adenoid cvstic carcinoma cells of salivary gland origin."Japanese Journal of Cancer Research. 90(3). 308-319 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yonemochi, H.: "Immunohistochemical localization of extracellular matrix molecules in complex odontoma."Dentistry in Japan. 35. 13-19 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 阿部真由美: "基底膜関連細胞外気質分子の免疫組織化学のための酵素処理の有効性とその応用"病理と臨床. 17(5). 515-520 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ida-Yonemochi, H.: "Xanthomatous lesion of the gingiva : a possible cause of delayed tooth eruption."Oral medicine & Pathology. 4(2). 79-84 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yonemochi, H.: "Pericoronal hamartomayous lesions in the opercula of teeth delayed in eruption : an immunohistochemical study of the extracellular matrix."Journal of Oral Pathology & Medicine. 27(8). 441-452 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Okuda, K.: "TGF-βinfluences early gingival wound healing in rats : an immunohistochemical evaluation of stromal remodelling by extracellular matrix molecules and PCNA."Journal of Oral Pathology & Medicine. 27(9). 463-469 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Irie, T.: "Intracellular transport of basement membrane-type heparan sulfate proteoglycan in adenoid cystic carcinoma cells of salivary gland origin : an immunoelectron microscopic study."Virchows Arch. 433(1). 41-48 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kimura, S.et al.: "Perlecan(heparan sulfate proteglycan)gene expression reflected in the characteristic histological architecture of salivary adenoid cystic carcinoma."Virchows Arch. 437(2). 122-128 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Murata, M.et al.: "Enamel proteins and extracellular matrix molecules are co-localized in the pseudocystic stroma space of adenomatoid odontogenic tumor."Journal of Oral Pathology & Medicine. 29(10). 483-490 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kimura, S.et al.: "Basement membrane heparan sulfate proteoglycan(perlecan)synthesized by ACC, adenoid cystic carcinoma cell of human salivary gland origin."Journal of Biochemistry. 25(2). 406-413 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toyoshima, K.et al.: "High-molecular-weight fibronectin synthesized by adenoid cystic carcinoma cells of salivary gland origin."Japanese Journal of Cancer Research. 90(3). 308-319 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yonemochi, H.et al.: "Immunohistochemical localization of extracellular matrix molecules in complex odontoma."Dentistry in Japan. 35. 13-19 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Abe, M.et al.: "The availability and application of enzymatic pretreatments in immunohistochemical study for basement membrane related extracellular matrix molecules."Pathology and Clinical Medicine. 17(5). 515-520 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ida-Yonemochi, H.et al.: "Xanthomatous lesion of the gingiva : a possible cause of delayed tooth eruption."Oral Medicine & Pathology. 4(2). 79-84 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yonemochi, H.et al.: "Pericoronal hamartomatous lesions in the opercula of teeth delayed in eruption : an immunohistocheimical study of the extracellular matrix."Journal of Oral Pathology & Medicine. 27(8). 441-452 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Okuda, K.et al.: "TGF-β influences early gingival wound healing in rats : an immunohistochemical evaluation of stromal remodeling by extracellular matrix molecules and PCNA."Jouranl of Oral Pathology & Medicine. 27(9). 463-469 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Irie, T.et al.: "Intracellular transport of basement membrane-type heparan sulfate proteoglycan in adenoid cystic carcinoma cells of salivary gland origin : an immunoelectron microscopic study"Virchows Arch. 433(1). 41-48 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kimura,S.: "Perlecan (heparan sulfate proteoglycan) gene expression reflected in the characteristic histological architecture of salivary adenoid cystic carcinoma."Virchows Arch. 437. 122-128 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Murata,M.: "Enamel protein s and extracellular matrix molecules are co-localized in the pseudocystic stroma space of adenomatoid odontogenic tumor."Journal of Oral Pathology & Medicine. 29. 483-490 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kimura,S.: "Basement membrane heparan sulfate proteoglycan (perlecan) synthesized by ACC, adenoid cystic carcinoma cell of human salivary gland origin."Journal of Biochemistry. 25. 406-413 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Toyoshima,K.: "High-molecular-weight fibronectin synthesized by adenoid cystic carcinoma cells of salivary gland origin."Japanese Journal of Cancer Research. 90. 308-319 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yonemochi,H.: "lmmunohistochemical localization of extracellular matrix molecules in complex odontoma."Dentistry in Japan. 35. 13-19 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Irie,T.: "Intracellular transport of basement membrane-type heparan sulfate proteoglycan in adenoid cystic carcinoma cells of salivary gland origin : an immunoelectron microscopic study."Virchows Arch. 433. 41-48 (1998)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kimura, S. et al.: "Basement membrane heparan sulfate proteglycan (perlecan) synthesized by ACC, adenoid cystic carcinoma cell of human salivary gland origin."Journal of Biochemistry. 25. 406-413 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toyoshima, K. et al.: "High-molecular-weight fibronectin synthesized by adenoid cystic carcinoma cells of sailvary gland origin."Japanese Journal of Cancer Research. 90. 308-319 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kimura, S. et al.: "Perlecan gene expression reflected in the characteristic histological architecture of salivary adenoid cystic carcinoma."Virchows Archiv. 45. 印刷中 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yonemochi, H. et al.: "Immunohistochemical localization of extracellular matrix molecules in complex odontoma"Dentistry in Japan. 35. 13-19 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 阿部 真由美ほか: "基底膜関連細胞外基質分子の免疫組織化学のための酵素処理の有効性とその応用"病理と臨床. 17. 515-520 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 豊島 公栄ほか: "癌実質と間質で異なるファイブロネクチン選択的スプライシング様式"日本病理学会会誌. 88. 307 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kimura, S.et al.: "Basement membrane heparan sulfate proteoglycan (perlecan) synthesized by ACC, adenoid cystic carcinoma cell of human salivary gland origin." Journal of Biochemistry. 25. 406-413 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toyoshima, K.et al.: "High-molecular-weight fibronectin synthesized by adenoid cystic carcinoma cells of salivary gland origin." Japanese Journal of Cancer Research. 90. 308-319 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Irie, T.et al.: "Intracellular transport of basement membrane type heparan sulfate proteoglycan in adenoid cystic carcinoma cells of salivary gland orgin: an immunoelectron microscopic study." Virchows Archiv. 433. 41-48 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Munakata, R.et al.: "Spindle cell carcinoma of the gingiva." Journal of Oral Pathology & Medicine. 27. 180-184 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yonemochi, H.et al.: "Pericoronal hamartomatous lesions in the opercula of teeth delayed in eruption: an immunohistochemical study of extracellar matrix." Journal of Oral Pathology & Medicine. 27. 441-452 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ono, Y.et al.: "Vascular invasion of O-1N, hamster squamous cell carcinoma with high potential of lymph node metastasis: Ultrastrucutural comparison between lymphatics and blood vessels." Pathology International. 48. 254-264 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-11-11  

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