| Project/Area Number |
10470382
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
HIURA Akio The University of Tokushima, School of Dentistry, Associate Prof., 歯学部, 助教授 (00106353)
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| Co-Investigator(Kenkyū-buntansha) |
KUWAHARA Michiyo The University of Tokushima, School of Dentistry, Assist., 歯学部, 助教授 (70243706)
NASU Fumio The University of Tokushima, School of Dentistry, Assist., 歯学部, 助手 (10180530)
石塚 寛 徳島大学, 歯学部, 教授 (50057494)
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| Project Period (FY) |
1998 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Synapses / Primary afferent neuron / Substantia gelatinosa / Interneuron / Neurophysiological study / Synapses / Primary afferent / Interneuron / Substantia gelatinosa / Spinal trigeminal nucleus / Svnapses / Primarv afferent / Spinal trigeminalnucleus |
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| Research Abstract |
The aim of this study is to identify the interneurons responding to the electrical stimuli peculiar to convey nociceptive information in the substantia gelatinosa of the mouse superficial dorsal horn. The second is to, investigate the synaptic relation of nociceptive primary afferent nerve (PAN) terminals to inhibitory interneurons such as GABAergic, thereby prove the flow of impulse from the PAN terminals to GABAergic interneurons. Taking out the spinal cord or medulla oblongata, the specimens were coronally cut at 500 μm thickness with microslicer and put into the chamber continuously circulated with medium (PH7.2 〜 7.4) adjusted the temperature at 37℃, including 95 % O_2 and 5 % CO_2.
Under the above ideal conditions, the glass electrode was inserted into the substantia gelatinosa. But now the resting potentials of the interneurons could not be obtained. Probably, the neurons were dying during the experiment. Thus, we have no data in this study, however, we have at least prepared the circumferential conditions to carry out the study. We should continue this study, overcome the problems and would get some of the interesting results in near future.
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