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Studies on functional roles of crystal-matrix protein interaction in developmental enamel mineralization

Research Project

Project/Area Number 10470385
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionThe Nippon Dental University, School of Dentistry

Principal Investigator

AOBA Takaaki  The Nippon Dental University, School of Dentistry at Tokyo, Department of Pathology, Professor, 歯学部, 教授 (30028807)

Co-Investigator(Kenkyū-buntansha) YAGISHITA Hisao  The Nippon Dental University, School of Dentistry at Tokyo, Department of Pathology, Lecturer, 歯学部, 講師 (50256989)
TAYA Yuji  The Nippon Dental University, School of Dentistry at Tokyo, Department of Pathology, Lecturer, 歯学部, 講師 (30197587)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1999: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1998: ¥6,900,000 (Direct Cost: ¥6,900,000)
KeywordsEnamel / Mineralization / Apatite / Crystal growth / Fluid environment / Degree of supersaturation / Matrix proteirts / Amelogenins / 溶液イオン組織
Research Abstract

Enamel formation is a complex biomineralization process, which comprises temporo-spatially orchestrated events, e.g., cellular proliferation and differentiation through the sequential epithelial-mesenchymal interactions, secretion of the tissue-specific matrix proteins and construction of their oligomeric molecular structures in the extracellular space, and precipitation/growth and three-dimonsionally oriented alignment of the largest carbonatoapatites in the body through the multiple interactions between organic and inorganic molecules. In comparison with dentinogenesis and osteogenesis, enamel formation is characterized by several unique features : (a) most of the secreted proteins are degraded in situ by multiple proteases and are removed from the tissue, (b) the growth of enamel crystals proceeds gradually through all the developmental stages, and (c) the mineralization process is not intervened by any remodeling of the formed tissue. These unique biominoralization processes are ex … More ecuted, at least in part depending on the tight cellular regulation of mass transport, e.g., transport of common ions (e.g, , Ca^<2+> and phosphate) and impurities (e.g., carbonate) from blood circulation into the extracellular space. In order to investinate the regulatory mechanism of biomineralization, our effort during the current period was focused on development of an experimental precipitation model by modifying a commercially available dialysis chamber. In designing the experimental apparatus, key considerations we took into account are : (a) utilizing a supersaturated solution as prodicted in in situ fluid phase, (b) coating apatite seeds with enamel proteins as found in the secretory enamel, and (c) limiting Ca supply into media surrounding the seed crystals as supposed in situ by previous autoradiographic work. The results obtained support the hypothesis that, under the situation where Ca supply becomes a rate-detormining step in the whole process, (i) the steady state condition with respect to the common ions is achievable in the mineralizing milieu and (ii) the resulting liquid composition depends in a sensitive manner on the presence of regulators for apatite precipitation, namely, enamel proteins and F-. Particularly, in relation to understanding of the pathogenosis of enamel fluorosis, which is now characterized by the delay of tissue maturation caused by excess incorporation of fluoride into the extracellular environment, the current work provided evidence for the effects of fluoride on Ca concentration in the mineralizing milieu. Less

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Hattori M, et al.: "Kinetic consideration of Ca transport and associated crystal precipitation in an in vitro precipitation model using dialysis membranes : fluoride-dependent regulation of Ca concentration in liquid environment."Jpn J Oral Biol. 43(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sato K, et al.: "FTIR spectroscopic studies of acid phosphate and carbonate in human enamel and synthetic carbonatoapatites."Odontology. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sato K, et al.: "Porcine male- and female-amelogenins and their putative roles in enamel mineralization."Odontology. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Amano T, et al.: "Soluble constituents in enamel fluid and their possible involvement in mineralization during porcine and rat amelogenesis."Jpn J Oral Biol. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yagishita H, et al.: "The secretion of amelogenins is associated with the induction of enamel and dentinoid in an ameloblastic fibro-odontoma."J Oral Pathol Med. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Aoba T, et al.: "Aggregation of 20 kDa porcine amelogenin in solution : FTIR and AFM analysis of the accompanying protein-conformational and morphological changes."Jpn J Oral Biol. 43(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 山崎雅光 他: "形成期エナメル質の成熟化の制御機構:結晶、基質蛋白、分解酵素の相互作用."歯科基礎誌. 43. 60-71 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hattori M, et al.: "Kinetic consideration of Ca transport and associated crystal precipitation in an in vitro precipitation model using dialysis membranes : fluoride-dependent regulation of Ca concentration in liquid environment."Jpn J Oral Biol. 43(in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sato K, et al.: "FTIR spectroscopic studies of acid phosphate and carbonate in human enamel and synthetic carbonatoapatites."Odontology. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sate K, et al.: "Porcine male- and female-amelogenins and their putative roles in enamel mineralization."Odontology. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Amano T, et al.: "Soluble constituents in enamel fluid and their possible involvement in mineralization during porcine and rat amelogenesis."Jpn J Oral Biol. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yagishita H, et al.: "The secretion of amelogenins is associated with the induction of enamel and dentinoid in an ameloblastic fibro-odontoma."J Oral Pathol Med. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Aoba T, et al.: "Aggregation of 20 kDa porcine amelogenin in solution : FTIR and AFM analysis of the accompanying protein-conformational and morphological changes."Jpn J Oral Biol. 43(in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamazaki M, et al.: "Mechanistic understanding of the maturation of developing enamel : Plausible interactions among crystals, matrix proteins and proteases."Jpn J Oral Biol. 43. 60-71 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hattori M, et al.: "Kinetic consideration of Ca transport and associated crystal precipitation in an in vitro precipitation model using dialysis membranes : fluoride-dependent regulation of Ca concentration in liquid environment."Jpn J Oral Biol. 43(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sato K, et al.: "FTIR spectroscopic studies of acid phosphate and carbonate in human enamel and synthetic carbonatoapatites."Odontology. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sato K, et al.: "Porcine male- and female-amelogenins and their putative roles in enamel mineralization."Odontology. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Amano T, et al.: "Soluble constituents in enamel fluid and their possible involvement in mineralization during porcine and rat amelogenesis."Jpn J Oral Biol. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yagishita H, et al.: "The secretion of amelogenins is associated with the induction of enamel and dentinoid in an ameloblastic fibro-odontoma."J Oral Pathol Med. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Aoba T, et al.: "Aggregation of 20 kDa porcine amelogenin in solution : FTIR and AFM analysis of the accompanying protein-conformational and morphological changes."Jpn J Oral Biol. 43(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 山崎雅光 他: "形成期エナメル質の成熟化の制御機構:結晶、基質蛋白、分解酵素の相互作用."歯科基礎誌. 43. 60-71 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Aoba T: "DISCOVERY! Voyage to the Land of Developmental Enamel Mineralization."J Dent Res.. 78・11. 1673-1676 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 青葉孝昭: "「今、エナメル質表層に何が起こっているか?」-脱灰と再石灰、この奇妙な現象を探る"日本歯科医師会雑誌. 52・7. 804-813 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y. Shimazu, et al.: "Functional significance of pyrophosphate in discrete forms in the mineralizing milieu."Jpn J Oral Biol.. 41・3. 193-201 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K. Sato, et al.: "Thermally-accelerated fluoridation of human enamel and dentin: plausible orderings of fluorine along the OH-column in apatite structure."J Nippon Dental University. 2. 36-41 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 青葉 孝昭: "生体の抗う蝕要因:再石灰化現象とう蝕病変の可逆性"日本歯科医学会雑誌. 18. 120-123 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K. Sato, et al.: "Carbonation of enamel apatite crystals: lattice substitutions and surface adsorption."Jpa J Oral Biol.. 41. 61-68 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Aoba, et al.: "Two discrete environments of calcium on the surface of enamel and synthetic apatites."Jpa J Oral Biol.. 41. 53-60 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kaori Sato,et al.: "Consideration of functional significance of dicarboxylic amino acids in calcium apatite precipitation." Jpn J Oral Biol. 40・6. 632-640 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshihito Shimazu,et al.: "Tissue Maturation of Fibro-osseous Lesions with Respect to the Inorganic Constituents and Crystallographic Features." Oral Med.Path.3. 61-66 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] N.Matsushima,et al.: "Small-angle X-ray scattering and computer-aided molecular modeling studies of 20 kDa fragment of porcine amelogenin: Does amelogenin adopt an elongated bundle structure?" J.Biochemistry. 123. 150-156 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 中原由絵、他: "日本人の永久歯にみられたう蝕病変の臨床診断と病理所見に基づく確定診断" 歯学. 86・2. 261-282 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 青葉孝昭(分担執筆)高木実 編: "口腔病理アトラス:う蝕" 文光堂, 20 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 青葉孝昭 著: "歯と歯周組織の病理学入門" 日本歯科大学病理学教室編, 255 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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