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Inhibitory effects of sugar alcohols on acid production of dental plaque bacteria

Research Project

Project/Area Number 10470387
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

YAMADA Tadashi  School of Dentistry, Tohoku Univ., Professor, 歯学部, 教授 (50005021)

Co-Investigator(Kenkyū-buntansha) IWAMI Yoshimichi  School of Dentistry, Tohoku Univ., Associate Professor, 歯学部, 助手 (60005030)
TAKAHASHI Nobuhiro  School of Dentistry, Tohoku Univ., Associate Professor, 歯学部, 助教授 (60183852)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥10,400,000 (Direct Cost: ¥10,400,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥9,300,000 (Direct Cost: ¥9,300,000)
KeywordsStreptococcus mutans / sugar metabolism / metabolism of sugar alcohols / sorbitol / xylitol / oxygen / inhibition of metabolism
Research Abstract

1. The aim of this study was to examine the effect of temporary exposure of the streptococcal cells to air on the intracellular flux of glucose and sorbitol metabolism by measuring acid excretion, fluorescence dependent on cellular level of NADH, glycolytic intermediates and enzyme activities. The exposure of cells to air decreased the acid excretions during glucose and sorbitol metabolism. The analysis of the glycolytic intermediates and the fluorescence suggested that the reduced level of acid excretion in the air-exposed glucose metabolizing cells resulted from the decrease in pyruvate catabolism. In the presence of sorbitol, the decreased acid production resulted from the reduced rates of the reactions catalyzed by sorbitol-phosphoenolpyruvate phosphotransferase and sorbitol 6-phosphate dehydrogenase because of shortage of substrates for these enzymes in addition to the decrease in pyruvate catabolism.
2. The aim of this study was to examine glucose and sorbitol metabolism in Strept … More ococcus mutans cells exposed and not exposed to air at the coexistence of these compounds by measuring acid excretion, levels of fluorescence dependent on cellular NADH and glycolytic intermediates. A part of bacterial cells grown under strictly anaerobic conditions (anaerobic cells) was exposed temporarily to air (aerobic cells). When glucose was added to the anaerobic cells metabolizing sorbitol, the acid excretion was increased. At the same time, the level of NADH was decreased and then reached to the higher plateau level than that during glucose metabolism. The aerobic cells neither metabolized sorbitol nor contained cellular intermediates. At 2 min after glucose was added in the presence of sorbitol, the acid excretion was started slowly and the intermediates appeared. The level of NADH was decreased at first and then increased. These results suggested that the anaerobic S. mutans cells metabolized glucose and sorbitol simultaneously, and that in the presence of sorbitol the aerobic cells could start to metabolize glucose by addition of glucose at 2 min after glucose was added, as the intermediates (PEP potential) for the glucose transport were accumulated. Less

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Y.Iwami et al.: "Rate-limiting steps of glucose and sorbitol metabolism in Streptococcus mutans cells exposed to air"Oral Microbiology and Immunology. 15(in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y.Iwami et al.: "Intracellular flux of glucose metabolism in streptococcal cells by simultaneous monitoring of fluorescence dependent on reduced nicotinamide adenine nucleotide and acid excretion under strictly anaerobic conditions"Oral Microbiology and Immunology. 14. 220-224 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H.Miyasawa et al.: "Growth-phase-and pH-dependent xylitol inhibition on acid production of Streptococcus mutans"Journal of Dental Research. 79(in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y.Iwami et al.: "Inhibition of sorbitol metabolism by air-exposure of Streptococcus mutans cells"Journal of Dental Research. 78. 346 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Iwami et al.: "Rate-limiting steps of glucose and sorbitol metabolism in Streptococcus mutans cells exposed to air"Oral Microbiology and Immunology. 15(in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Iwami et al.: "Intracellular flux of glucose metabolism in streptococcal cells by simultaneous monitoring of fluorescence dependent on reduced nicotinamide adenine nucleotide and acid excretion under strictly anaerobic conditions"Oral Microbiology and Immunology. 14. 220-224 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Miyasawa et al.: "Growth-phase- and pH-dependent xylitol inhibition on acid production of Streptococcus mutans."Journal of Dental Research. 79(in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Iwami et al.: "Inhibition of sorbitol metabolism by air-exposure of Streptococcus mutans cells"Journal of Dental Research. 78. 346 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y.Iwami: "Rate-limiting steps of glucose and sorbitol metabolism in Streptococcus mutans cells exposed to air"Oral Microbiology and Immunology. 15(in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Miyasawa: "Growth-phase- and pH-dependent xylitol inhibition on acid production of Streptococcus mutans"Journal of Dental Research. 79(in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 宮澤はるみ: "Xylitol による mutans streptococci の酸産生阻害効果:増殖条件とpHの影響"東北大学歯学雑誌. 19(1)(印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 岩見憙道: "ソルトビールおよびキシリトールによる Streptococcus mutans のグルコース代謝阻害とその機構"歯科基礎医学会雑誌. 41(5). 480 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y. Iwami: "Inhibition of sorbitol metabolism by air-exposure of Streptococcus mutans cells"Journal of Dental Research. 78. 346 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 岩見 憙道: "Streptococcus mutansのソルビトール代謝の酸素による阻害段階" 歯科基礎医学会雑誌. 40・S. 459 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.IWAMI: "Inhibition of sorbitol metabolism by air-exposure of Streptococcus mutans cells" Journal of Dental Research. 78・S. 346 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] N.TAKAHASHI: "Acid-induced acid tolarance and acidogenicity of non-mutans streptococci" Oral Microbiology and Immunology. 14・1. 43-48 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] N.TAKAHASHI: "Effects of pH on the glucose and lactate metabolisms by the washed cells of Actinomyces nacslundii under anaerobic and aerobic conditions." Oral Microbiology and Immunology. 14・1. 60-65 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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