| Project/Area Number |
10470389
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
TAKIGAWA Masaharu Okayama University, Dental School, Professor, 歯学部, 教授 (20112063)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIDA Takashi Okayama University, Dental School, Research Assistant, 歯学部, 教務員 (30322233)
HATTORI Takako Okayama University, Dental School, Instructor, 歯学部, 助手 (00228488)
NAKANISHI Tohru Okayama University, Dental School, Associate Professor, 歯学部, 助教授 (30243463)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2000: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 1999: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1998: ¥5,300,000 (Direct Cost: ¥5,300,000)
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| Keywords | ecogenin / CTGF / growth factor / chondrocytes / proliferation / differentiation / matricrine / receptor / phosphorylation / MAPK / ELK / 血管内皮細胞 / 骨芽細胞 / 情報伝達 |
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| Research Abstract |
1) Specific receptors for ecogenin/CTGF were found on chondrocytes, osteoblasts and vascular endothelial cells. Its molecular weight was about 240 kDa.
2) Specific binding of radiolabeled ecogenin/CTGF for chondrocytes decreased as the cells differentiated.
3) In chondrocyte culture system, ecogenin/CTGF bound cell surface heparan sulfate proteoglycans after secreted and was then released, suggesting that it is a matricrine factor.
4) Ecogenin/CTGF stimulated phosphorylation of ERK and p38MAPK in chondrocytes. Using specific inhibitors, we found that ecogenin/CTGF promoted the proliferation and differentiation of chondrocytes through ERK and p38MAPK pathways, respectively.
5) Two binding proteins for ecogenin/CTGF were purified from human chondrocytic cell line HCS-2/8. The one was 42 kDa protein of which N-terminal amino acid sequence corresponded to that of γ-actin and the other was 50 kDa protein of which N-terminal amino acid sequence corresponded to that of cytokeratin.
6) When ecogenin/CTGF was overexpressed in Cos-7 cells, it localized around centrosome. C-terminal fragment was also found in cells.
These findings suggest that ecogenin/CTGF not only acts as a paracrine and matricrine factor through its specfic receptors but also functions through an alternative intracellular pathway.
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