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Effect of hepatocyte growth factor/scatter factor on the development and metabolism of bone tissue : relation of regulation on fetuin gene expression.

Research Project

Project/Area Number 10470391
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionKagoshima University

Principal Investigator

DAIKUHARA Yasushi  Kagoshima University, Dental School, Biochemistry, Professor, 歯学部, 教授 (40028733)

Co-Investigator(Kenkyū-buntansha) AMITA YASUDA Yoko  Kagoshima University, Dental Hospital, Biochemistry, Research Assistant, 歯学部, 教務職員 (70274850)
KOKUBO Akiko  Kagoshima University, Dental School, Biochemistry, Research Associate, 歯学部, 助手 (90304534)
KAJIHARA Takehiro  Kagoshima University, Dental School, Biochemistry, Research Associate, 歯学部, 助手 (10305138)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 2000: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1999: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1998: ¥5,800,000 (Direct Cost: ¥5,800,000)
KeywordsHepatocyte growth factor / Scatter factor / c-Met / Fetuin / First branchial arch / Bone resorption / Mandible / Development of tongue / myo D / myogenin / 細胞遊走 / myoD / myf5 / フェチェイン / 骨発生 / 骨芽細胞 / 骨形成タンパク
Research Abstract

In order to study whether and how hepatocyte growth factor/scatter factor (HGF/SF) is involved in development of bone tissue, we analyzed mRNAs for HGF/SF and c-Met, the receptor for HGF/SF, in the forelimb, hindlimb, somite and first branchial arch from mice of embryonic day 10 (E10) by reverse transcription-polymerase chain reaction and found that HGF/SF and c-Met were expressed in these tissues. We also confirmed the expression of HGF/SF and c-Met in these tissues from E10 to E16 mice by whole-mount in situ hybridization using RNA probes and found that expression of c-Met was especially high at the position of ossification. This finding suggested that HGF/SF may regulate the differentiation of osteoblasts ht bone tissue. Although we could not detect the expression of fetuin at any stages, above results also suggested that HGF/SF may involved in the development of tongue. Therefore, we next cultured the first branchial arch from E10 mice with serum-free EGJb medium for 120 hr in vitro and found that c-Met expressed at the position where mandible will develop. We also found that muscle specific transcription factors, such as myoD, myogenin and myf5, expressed in the cultured first branchial arch described above at the position of tongue. Addition of recombinant HGF/SF to the medium at the concentration of 50 ng/ml enlarged the size of tongue after 120 hr incubation, and the position of high proliferation of muscle cells of tongue was corresponding with that of c-Met was expressed. These findings suggested that HGF/SF suppressed the differentiation muscle of tongue during developing stage and at the same time, HGF/SF stimulated proliferation of myoblasts of tongue by paracrine mode.
During this project was processing, we also found and reported that rat fetuin stimulated bone resorption in the presence of parathyroid hormone and that rat fetuin was involved development of neocortex in fetal rats.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Arakaki,N.: "Hepatocyte growth factor/scatter factor activates the apoptosis signaling pathway by increasing caspase-3 activity in Sarcoma 180 cells."Biochem.Biophys.Res.Commun.. 245. 211-215 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kazi,J.A.: "Changes with age in rat fetuin concentration in serum and its mRNA expression."J.Biochem.. 124. 179-186 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Arakaki,N.: "Involvement of oxidative stress in tumor cytotoxic activity of hepatocyte growth factor/scatter factor."J.Biol.Chem.. 274. 13541-13546 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kajihara,T.,: "Expression of hepatocyte growth factor/scatter factor and c-Met in human dental papilla and fibroblasls from dental papilla."Arc.Oral Biol.. 44. 135-147 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Musa,D.M.A.A.: "Involvement of a cis-acting element in the suppression of carbamoylphosphate synthetase I gene expression in the liver of carnithine-deficient mice."Mol.Gen.Metab.. 68. 346-356 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakamura,O.: "Effect of rat fetuin on stimulation of bone resorption in the presence of parathyroid hormone."Biosci.Biotechnol.Biochem.. 63. 1383-1391 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ohnishi,T.: "Prostaglandin E2 predominantly induces production of hepatocyte growth factor/scatter factor in human dental pulp in acute inflammation."J.Dent.Res.. 79. 748-755 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tamura,M.: "Structure and function of hepatocyte growth factor/scatter factor (HGF/SF)."Current Topics in Biochem.Res.. 2. 149-159 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 田村正人: "HGF(肝細胞増殖因子)"蛋・核・酵、. 45. 1152-1157 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Dziegielewska,K.M.: "Fetuin in the developing neocortex of the rat : distribution and origin."J.Comp.Neurol.. 423. 373-388 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 中村修(分担): "タンパク質化学 第5巻(I)(血漿タンパク質)"廣川書店(印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tamura,M.: "Structure and function of hepatocyte growth factor/scatter factor (HGF/SF)."Current Topics in Biochem.Res.. 2. 149-159 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Dziegielewska,K.M.: "Fetuin in the developing neocortex of the rat : distribution and origin."J.Comp.Neurol.. 423. 373-388 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 中村修(分担): "タンパク質化学 第5巻(I)(血漿タンパク質)"廣川書店(印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Arakaki,N.: "Involvement of oxidative stress in tumor cytotoxic activity of hepatocyte growth factor/scatter factor."J.Biol.Chem.. 274. 13541-13546 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kajihara,T.: "Expression of hepatocyte growth factor/scatter factor and c-Met in human dental papilla and fibroblasts from dental papilla."Arc.Oral Biol.. 44. 135-147 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Musa,D.M.A.A.: "Involvement of a cis-acting element in the suppression of carbamoylphosphate synthetase I gene expression in the liver of carnithine-deficient mice."Mol.Gen.Metab.. 68. 346-356 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nakamura,O.: "Effect of rat fetuin on stimulation of bone resorption in the presence of parathyroid hormone."Biosci.Biotechnol.Biochem.. 63. 1383-1391 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ohnishi,T.: "Prostaglandin E2 predominantly induces production of hepatocyte growth factor/scatter factor in human dental pulp in acute inflammation."J.Dent.Res.. 79. 748-755 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Dziegielewska,K.M.: "Fetuin in the developing rat brain-distribution in neocortex,concentration in plasma and cerebrospinal fluid."J.Comparative Neurology. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kajihara,T.: "Expression of hepatocyte growth factor/scatter factor(HGF/SF)and c-met in human dental papilla and fibroblasts from dental papilla." Arch.Oral Biol.(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Arakaki,N.: "Hepatocyte growth factor/scatter factor activates the apoptosis signaling pathway by increasing caspase-3 activity in Sarcoma 180 cells." Biochem.Biophys.Res Commun.245. 211-215 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kazi,J.A.: "Changes with age in rat fetuin concentration in serum and its mRNA expression." J.Biochem.124. 179-186 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-11-11  

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