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Molecular cloning of transcription factor for odontblast differentiation

Research Project

Project/Area Number 10470407
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

AKAMINE Akifumi  Kyushu Univ., Dentistry, Professor, 歯学部, 教授 (00117053)

Co-Investigator(Kenkyū-buntansha) NAKASHIMA Kisako  Kyushu Univ., Dentistry, Research Associate, 歯学部, 助手 (20207773)
GORO Yasuharu  Kyushu Univ., Dentistry, Research Associate, 歯学部, 助手 (00170473)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥12,200,000 (Direct Cost: ¥12,200,000)
Keywordsdentinogenesis / Transcription factor / molecular cloning / Gli / Zinc finger protein / bone morphogenetic protein / Growth / differentiation factor / gene targeting / ジンクフィンガ-プロテイン
Research Abstract

We have cloned a new member of the zinc finger transcription factor, G23/Gli5 from incisor pulp RNA by RT-PCR using degenerate primers. The five zinc finger domain encoded by mouse G23 has almost 65% amino acid sequence homology with Gli1, Gli2 and Gli3. Northern blot analysis revealed G23/Gli5 is expressed in dental pulp, kidney and testis. In situ hybridization of sections and while mount embryos demonstrated G23/Gli5 is first detected diffusely in forelimb and hindlimb bud at 10.0 days post coitus (dpc). At 10.5 dpc, it is expressed in the branchial arches, limb bud, craniofacial border, and ventral part of the tail. Later, it is expressed in whisker follicle, intervertebral disc, kidney, and testis. In tooth germ, G23/Gli5 is expressed in dental papillae at bell stage, suggesting that it might function as a repressor during odontoblast differentiation.
We made targeted disruption of G23/Gli5. The second exon, 850bp from 5'-untranslated region to 3rd zinc finger DNA binding domain, containing the start codon, ATG was replaced by LacZ polyA and the lox-neo-polyA cassette. Homologous recombination was confirmed by Southern blot and by PCR. The homozygotes were fully viable and did not seem to have any conspicuous phenotype. The complementary expression patterns of G23/Gli5 and Gli1 family members suggest the redundant function of G23/Gli5 and Gli1 members. We are now analyzing the double homozygotes to find the function of G23/Gli5 during tooth development.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Nakashima M., Murakami T. and Akamine A.: "Transforming growth factor b SUPERFAMILY MEMBERS EXPRESSED IN RAT INCISOR PULP."Archs oral Biol.. 43・9. 745-751 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakashima M., Murakami T. and Joyner A.: "Expression of growth/differentitaion factor 11, a new member of the BMP/TGF b superfamily during mouse embryogenesis."Mech. Dev.. 80・2. 185-189 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakashima M., Toyono T., Murakami T., and Akamine A.: "Transforming growth factorβ superfamily members expressed in rat incisor pulp"Archs oral Biol.. 43(9). 745-751 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakashima M., Toyono T.,Akamine A., and Joyner A.: "Expression of Growth/differentiation factor 11, a new member of the BMP/TGFβ Superfamily during mouse embryogenesis"Mech. Dev.. 80(2). 185-189 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakashima M.,Toyono T.,Murakami T.and Akamine A.: "Transforming growth factorβ superfamily members expressed in rat incisor pulp."Archs oral Biol.. 43・9. 745-751 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nakashima M.,Toyono T.,Akamine A.and Joyner A.: "Expression of growth/differentiaion factor 11,a new member of the BMP/TGFβ Superfamily during mouse embryogenesis."Mech.Dev.. 80・2. 185-189 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Nakashima, T.Toyono, T.Murakami A.Akamine: "Transforming growth factor- β superfamily members expressed in rat incisor pulp." Archs oral Biol.43・9. 745-751 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Nakashim, T.Toyono, A.Akamine, A.Joyner: "Expression of growth/differentiaton factor 11, a new member of the BMP/TGF β superfamily during mouse embryogenesis." Mech. Dev.80・2. 185-189 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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