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Development of new pulp capping/ amputation agent combined with mineral induction activity

Research Project

Project/Area Number 10470408
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionHealth Sciences University of Hokkaido

Principal Investigator

MATSUDA Koichi  Health Sciences University of Hokkaido School of Dentistry, Department of Operative Dentistry and Endodontology, Professor, 歯学部, 教授 (20109458)

Co-Investigator(Kenkyū-buntansha) SAITO Takashi  Health Sciences University of Hokkaido School of Dentistry, Department of Operative Dentistry and Endodontology, Assistant Professor, 歯学部, 講師 (40265070)
TUKAGOSH Shin  Health Sciences University of Hokkaido School of Dentistry, Department of Operative Dentistry and Endodontology, Instructor, 歯学部, 助手 (10236846)
ENDO Kazuhiko  Health Sciences University of Hokkaido School of Dentistry, Department of Dental Materials Science, Associate Professor, 歯学部, 助教授 (70168821)
TOYOOKA Hiroki  Health Sciences University of Hokkaido School of Dentistry, Department of Operative Dentistry and Endodontology, Instructor, 歯学部, 助手 (30285530)
中出 修  北海道医療大学, 歯学部, 講師 (70188986)
Project Period (FY) 1998 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥15,200,000 (Direct Cost: ¥15,200,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1999: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1998: ¥8,400,000 (Direct Cost: ¥8,400,000)
KeywordsBMP / Mineral Induction / Pulp Capping / Pulp Amputation / 象牙芽細胞 / 断髄剤 / rhBMP-2 / コラーゲン / 多孔質ハイドロキシアパタイト / phosphophoryn / BMP / 石灰化 / 象牙質誘導
Research Abstract

The final goal of this project is to develop new bio - active pulp capping agent using rhBMP-2. First, we examined the effects of rhBMP-2 on differentiation of human pulp cells by measurement of, mRNA expression using quantitative PCR method. We got pulp tissue from extracted fresh wisdom tooth, and obtained pulp cells by out-growth method. We cultured the pulp cells with rhBMP-2 of 0, 10, ; 100 and 1000 ng/ml without serum, and measured various mRNA expressions using LightCycler (Roche, Germany). As a result, rhBMP-2 enhanced dentin sialo phosphoprotein mRNA expression that is thought to be marker of odontoblast differentiation, in a concentration pendent manner. Therefore, we found that rhBMP-2 enhanced the differentiation of pulp cells into odontoblasts, and it is suggested that effective pulp-conservative agent would be produced by using rhBMP-2. Next, We implanted rhBMP-2/ type I collagen/ hydroxyapatite/ phosphophoryn composite into subcutaneous connective tissue or onto skull of rat. Then we sacrificed the rats, and analyzed the implants biochemically and histologically. As a result, we found that rhBMP-2/ type I collagen/ hydroxyapatite/ phosphophoryn composite induced bone formation strongly compared with control group. It was Indicated that rhBMP-2/ type I collagen/ hydroxyapatite/ phosphophoryn composite can be effective pulp capping agent.

Report

(5 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (54 results)

All Other

All Publications (54 results)

  • [Publications] T.Saito et al.: "Apatite induction by insoluble dentin collagen"J. Bone and Mineral Res.. 13. 265-270 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 遠藤 一彦: "生体材料の表面処理"表面技術. 49. 676-681 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Matsuda et al.: "In vivo corrosion and tarnish of dental Au-based and Ag-based alloy restorations"J. Esthetic Dent.. 11. 58-66 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Kawakami et al.: "Application of Er : YAG laser to composite resin restoration in anterior teeth"J. Esthetic Dent.. 11. 80-85 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Fujii et al.: "Scanning electron microscopic study of the effects of Er : YAG laser on root cementum"J. Periodont.. 69. 1283-1290 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 小山 宏樹: "正常ヒト骨芽細胞におけるBMP産生に及ぼすフェニトインの効果"東日本歯学雑誌. 18. 39-48 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 豊岡 広起: "遊離型ホスホホリンによる石灰化誘導"日本歯科保存学雑誌. 42. 640-646 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Ito et al.: "In vitro mineral induction by immobilized phosphoprotein -Effect of phosphate group on interfacial tension for mineral induction-"Higasi Nippon Dental Journal. 18. 7-16 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 斎藤 隆史: "象牙質基質モデルとしてのフォスフォフォリン-再構成コラーゲン線維複合体による石灰化誘導"日本歯科保存学雑誌. 42. 776-782 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 斎藤 隆史: "In vitroにおける不溶性象牙質コラーゲンによる石灰化誘導 -リン酸基が界面エネルギーに及ぼす影響-"日本歯科保存学雑誌. 42. 323-329 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 塚越 慎: "オブチュラIIによる根管充填の充塞性"日本歯科保存学雑誌. 44. 763-767 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Saito et al.: "In vitro apatite induction by phosphophoryn immobilized on modified collagen fibrils"J. Bone & Mineral Res.. 15. 1615-1619 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koyama et al.: "Evidence for the involvement of bone morphogenetic protein-2 in phenytoin-stimulated osteocalcin secretion in human bone cells"Arch. Oral Biol.. 45. 647-655 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 斎藤 隆史: "リンタンパク質-再構成コラーゲン線維複合体による石灰化誘導-コラーゲン線維構造が石灰化に及ぼす影響-"日本歯科保存学雑誌. 43. 826-831 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Endo et al.: "Corrosion characteristics of ferric and austenitic stainless steels for dental magnetic attachment"Dental Mater. J.. 19. 34-49 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Hashimoto et al.: "In vivo degradation of resin-dentin bonds in humans over 1 to 3 years"J. Dent. Res. 19. 1385-1391 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 伊藤 修一: "In vitroにおける結合型リンタンパク質による石灰化誘導-リンタンパク質濃度が界面張力に及ぼす影響-"日本歯科保存学雑誌. 44. 79-85 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Yamane et al.: "Mechanism of adhesion between 4-META resin and alloys based on bolgers acid-based interaction"Dent. Material J.. 20. 63-74 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 斎藤 隆史: "脱灰象牙質コラーゲンのin vitro石灰化に対する 2-Hydroxylethyl Methacrylate(HEMA)の影響"日本歯科保存学雑誌. 44. 630-636 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 島袋 智広: "水酸化カルシウム系根管貼薬剤の応用に関する基礎的研究2. 水酸化カルシウム系製剤の応用における根管充填の封鎖性"日本歯科保存学雑誌. 44. 22-27 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Hashimoto et al.: "The extent to which resin can infilterate dentin by acetone-based adhesives"J. Dent. Res.. 81. 74-78 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Saito, M. Yamauchi MA, Crenshaw: "Apatite induction by insoluble dentin collagen"J. Bone & Miner. Res.. 13. 165-270 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Kawakarai, Y. Ibaraki, T. Saito, K. Matsuda: "Application of Erbium : YAG laser to comnosite resin restoration in anterior teeth"J. Esthetic Dent.. 11. 80-85 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Matsuda, K. Endo, K. Yamada, H. Ohn: "In vivo corrosion and tarnish of dental Au-based and Ag-based alloy restoration"J. Esthetic Dent.. 11. 58-66 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Endo, H. Ohno: "Surface modification of biomaterials"Surface Technique. 49. 676-681 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Fufii, CB. Pierre, O. Kanal, T. Kawakami, K. Matsuda, Y. Kowashi: "Scanning electron microscopic study of the effects of Er : YAG laser on root cementum"J. Periondont. 69. 1283-1290 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Saito, H. Toyooka, S. Ito, MA. Crenshaw, K. Matsuda: "In vitro mineral induction by insoluble dentin collagen -Effect of phosphate group on interfacial energy for mineral induction-"Jpn. J. Conservative Dent. 42. 323-329 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Saito, Y. Abiko, S. Ito, MA Grenshaw, K.Matsuda, Y.Yamauchi, K.Koichi: "Apatite induction by phosphophoryn-reconstuted collagen fibrils complex as a model dentin collagen"Jpn. J. Conservative Dent. 42. 776-782 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Toyooka, T. saito, MA. Crenshaw, S. Ito, K. Matsuda: "Regulation of mineralization by free-type phosphophoryn in dentin"Jpn. J. Conservative Dent.. 42. 640-646 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Ito, T. saito, MA. Crenshaw, E Toyooka, K.Matsuda: "In vitro mineral induction by immobilized phosphoprotein -Effect of phosphate group on inter facial tension for mineral induction-"Higashi Nippon Dental J.. 18. 7-15 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Koyama, O. Nakade, T. Saito, T. Kaku: "Effects of phenytoin on synthesis of bone morphogenetic protein (BMPs) in normal human bone cells in vitro"Higashi Nippon Dental J.. 18. 39-48 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Tsukagoshi, Y. Ibaraki, Enakawaki, T. Sato, Y. Tsuji, M. Hiramoto, K. Haraguchi, K. Matsuda: "Root canal filling by Obtura II"Jpn. J. Conservative Dent.. 42. 763-767 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Saito, M. Yaraauchi, Y. Abiko, K. Matsuda, MA. Crenshaw: "In vitro apatite induction oy phosphophoryn immobilized on modified collagen"J. Bone & Miner. Res.. 15. 1615-1619 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Koyama, O. Nakade, T. Saito, T. Takuma, Y. Kaku: "Evidence for the involvement of BMP-2 in phenytoin-stimulated osteocalcin secretion in human bone cells"Arch.Oral Biol.. 45. 647-655 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Saito, T. Uemura, S. Ito, T. Fujii, MA. Crenshaw, K. Matsuda: "Mineral induction phosphoprotein-reconstituted collagen fibrils complex -Effect of collagen fibril structure on mineral induction-"Jpn. J. Conservative Dent.. 143. 826-831 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Endo, M. Suzuki, H. Ohno: "Corrosion characteristics of ferric and austentic stainless steels for dental magnetic attachment"Dental Materials J.. 19. 34-49 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Hashimoto, Rohno, M. Kara, K. Endo. H. Sano. H. Oguchi: "In vivo degradation of resin-dentin bonds in humans over 1 to 3 years"J. Dent. Res.. 79. 1385-1391 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y. Yamane, H. Ohno, K. Endo: "Mechanism of adhesion between 4-META resin and alloy besed on Bolger's acid-base interaction"Dent. Mater. J.. 20. 63-74 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Shiraabukuro, S. Tsukagoshi, M. Imakita, Y. Ibaraki, K.Matsuda: "Fundamental stud on application of calcium hydro ? Oxide to root canal filling"Jpn. J. Conservative Dent. 44. 22-27 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Ito, T. Saito H. Toyooka, MA. Crenshaw, K. Matsuda: "Mineral induction by immobilized phosphoprotein -Effect of phosphoprotein concentration on interfacial tension-"Jpn. J. Conservative Dent. 44. 79-85 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Saito, S. Nakabo, J. Doi, Y. Nishitani, S. Ito, K. Matsuda, M. Yoshiyama: "Effect of 2-Hydroxyethyl Methacrylate (HEMA) on mineral induction by dematerialized dentil collagen in vitro"Jpn. J. Conservative Dent.. 44. 630-636 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Hashimoto, H. Ohno, M. Kaga, H. Sano, K. Endo, H. Oguchi: "The extent to which resin can infilterate dentin by acetone-based adhesives"J. Dent. Res.. 81. 74-78 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 斎藤 隆史: "脱灰象牙質コラーゲンのin vitro石灰化に対する2-hydroxyethyl Methacrylate(HEMA)の影響"日本歯科保存学雑誌. 44・3. 630-636 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 伊藤 修一: "In vitroにおける結合型リンタンパク質による石灰化誘導-リンタンパク質濃度が界面張力に及ぼす影響-"日本歯科保存学雑誌. 44・1. 79-85 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] TAKASHI SAITO: "In vitro apatite induction by phosphophoryn immobilized on Modified collagen fibrils"Journal of Bone & Mineral Research . 15・8. 1615-1619 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 斎藤隆史: "リンタンパク質・再構成コラーゲン線維複合体による石灰化誘導"日本歯科保存学雑誌. 43・4. 826-831 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 伊藤修一: "In vitroにおける結合型リンタンパク質による石灰化実験"日本歯科保存学雑誌. 44・1. 79-85 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.KOYAMA: "Evidence for the involvement of BHP-2 in phenytoin-stimulated osteocaloin Secretion in human bone cells"Arch.Oral,Biology. 45. 647-655 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 斎藤 隆史 ら: "in vitroにおける象牙質コラーゲンによる石灰化誘導実験"日本歯科保存学会雑誌. 42・2. 323-329 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S.Ito et al.: "In Vitro Mineral Induction by Immobilized Phosphoprotein"Higashi Nippon Dental Journal. 18・1. 7-15 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 豊岡 広起 ら: "遊離型フォスフォフォリンによる象牙質石灰化の制御"日本歯科保存学会雑誌. 42・3. 640-646 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 斎藤 隆史 ら: "象牙質基質モデルとしてのフォスフォフォリン-再構成コラーゲン線維複合体による石灰化誘導"日本歯科保存学会雑誌. 42・4. 776-782 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Sito et al.: "In Vitro Apatite Induction by Phosphophoryn Immobilitized on Modified Collagen"Journal of Bone Mineral Research. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 斎藤隆史: "in vitroにおける象牙質コラーゲンによる石灰化誘導" 日本歯科保存学雑誌. 42(2). 323-329 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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