| Project/Area Number |
10470417
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
KANYAMA Manabu (1999) Okayama University, Dental School, Research, 歯学部, 助手 (90294420)
山下 淳 (1998) 岡山大学, 歯学部, 教授 (00066995)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Kouji Okayama University, Dental Hospital, Lecture, 歯学部・附属病院, 助手 (30304322)
KUBOKI Takuo Okayama University, Dental Hospital, Lecture, 歯学部・附属病院, 講師 (00225195)
TAKIGAWA Masaharu Okayama University, Dental School, Professor, 歯学部, 教授 (20112063)
完山 学 岡山大学, 歯学部, 助手 (90294420)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥11,500,000 (Direct Cost: ¥11,500,000)
Fiscal Year 1999: ¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1998: ¥4,100,000 (Direct Cost: ¥4,100,000)
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| Keywords | odontoblast / adenovirus vector / dental pulp / subtractive hybridization / LacZ gene / 歯質保全療法 / subtractive hybridization |
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| Research Abstract |
This study first sought to find unknown genes that expressed specifically to be made the restorative dentine by odontblast. Next, to confirm that foreign genes can be transferred to dental pulp of rats and to clarify the in vivo transfer availability of the adenovirus vectors.
When tooth was prepared the cavity by diamond points, we detected the specific 14 clones from dental pulp by subtractive hybridization.
Recombinant adenovirus harboring LacZ gene was injected into the prepared tooth cavity of 6-week-old Wistar rats. At 1week after injection, the tooth was dissected and X-gal staining examined the expression of delivered LacZ. To investigate the expression of transferred gene in other organs, total RNA was extracted from liver, kidney, heart, and brain and expression of LacZ mRNA were analysed by RT-PCR. Expression of LacZ was observed a few odontblasts in the cavity. In the other organs, expression of the delivered transgene was not observed. Based on these findings, direct gene delivery into the tooth cavity using adenovirus vector is feasible as an effective in vivo method.
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