Factors involved in the interactions, of Candida albicans and oral bacteria
| Project/Area Number |
10470419
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | Hiroshima University |
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Principal Investigator |
HAMADA Taizo Hiroshima University, Faculty of Dentistry, Professor, 歯学部, 教授 (50034244)
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| Co-Investigator(Kenkyū-buntansha) |
MAKIHIRA Seicho Hiroshima Univ., Faculty of Dent., Research Associate, 歯学部・附属病院, 助手 (80304450)
MISHIMURA Masahiro Hiroshima University, Faculty of Dentistry, Research Associate, 歯学部, 助手 (00294570)
NIKAWA Hiroki Hiroshima University, Faculty of Dentistry, Dental Hospital, Lecturer, 歯学部・附属病院, 講師 (10228140)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1999: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1998: ¥3,900,000 (Direct Cost: ¥3,900,000)
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| Keywords | Candida albicans / Streptococcus / Actinomyces / Lactobacillus / hydroxylapatite / Type I collagen / aggregation / plaque flora / aggregation / Streptococcus mutans / Streptococcus sanguis / plaque formation / cariogenicity |
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| Research Abstract |
Objective : Several recent reports imply the possibility of cariogenicity of denture plaque containing C. albicans. Hence the purpose of this study was first to investigate the effects of salivary and serum pellicles on C. albicans and Strep. mutans colonization on hydroxylapatite beads, and second to investigate the effects of salivary and serum pellicles on C. albicans , Streptococcus mutans Strep. sanguis Lactobacillus and Actinomyces colonization on type I collagen. Further, interactions between oral flora and Candida albicans is of importance in the (denture) plaque formation. Finally we also investigated to quantify the coadherence of C. albicans and oral bacteria and the effects of dietary sugar on the coadherence. Results & conclusions : When the relative ATP content of the 48h colonization of both isolates of Strep. mutans were compared, a saliva pellicle was significantly more effective in promoting bacterial colonization than either uncoated or serum pellicle (ANOVA ; p<0.01
… More
). In contrast, in the case of colonization of C. albicans isolates a serum pellicle was significantly more effective in promoting the colonization of C. albicans GDH 18 and GDH 19, than both uncoated specimens and saliva pellicle (ANOVA ; p<0.01). Similarly, with mutans streptococci, a saliva pellicle was significantly more effective in promoting bacterial colonization compared with the pellicle free collagen disk, and the serum-coated sample significantly inhibited the colonization of streptococci (ANOVA ; p<0.01). In contrast, in the case of C. albicans, Lactobacillus and Actinomyces isolates, a serum pellicle was significantly more effective in promoting the colonization, followed by saliva-pellicle and uncoated specimen (ANOVA ; p<0.01). These resulta imply that crevicular fluid rich in seruminous components would promote the colonization of Candida, lactobacilli and Actinomyces on type I collagen surfaces as opposed to streptococci which showed greater avidity to saliva-coated surfaces. The phenomena that when the yeasts were grown in media supplemented with high concentration of galactose, they lost the ability to adhere with the isolates of Strep. sanguis, and acquired the ability to adhere with one isolate of Strep. sobrius, Strep. salivarius and Actinomyces, should be contributed to the survival or spreading of the fungi in the oral tissue surfaces, since the networks of intergeneric coaggregations are considered to play an important role in the ecology of dental plaque. Less
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Report
(3 results)
Research Products
(7 results)
