| Project/Area Number |
10470427
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Chiba University |
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Principal Investigator |
TANZAWA Hideki Chiba University, School of Medicine, Professor, 医学部, 教授 (50236775)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOE Hidetaka Chiba University, University Hospital, Associate Professor, 医学部, 講師 (70261930)
SHIRASAWA Hiroshi Chiba University, School of Medicine, Professor, 医学部, 教授 (00216194)
SUZUKI Nobuo Chiba University, School of Medicine, Professor, 医学部, 教授 (90111426)
IMAI Yutaka Dokkyo University, Dept.of Oral Surgery, Associate Professor, 医学部, 助教授 (80114239)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 2000: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1999: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1998: ¥8,300,000 (Direct Cost: ¥8,300,000)
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| Keywords | 13q14.3 / differential display / loss of heterozygosity (LOH) / lymph node metastasis / tumor suppressor genes / representational difference analysis |
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| Research Abstract |
We studied tumors from 54 unrelated patients with oral SCC by the polymerase chain reaction (PCR) -loss of heterozygosity (LOH) assay, using 18 different polymorphic loci. The results enabled the identification of a putative minimal region of deletion mapped at 13q 14.3. The commonly deleted region is located close, but telomeric to the RBI locus. We also examined the same samples for inactivation and mutation of the RBI gene by immunohistochemical analysis, PCR-single-strand conformational polymorphism (SSCP) analysis to detect any mutation of the RBI gene. However, neither mutation nor inactivation of the RBI gene was detected in our samples. Interestingly, we found significant correlation between LOH of 13q14.3 and lymph node metastasis. These results suggest the existence of a new suppressor gene near D13S273-D13S176 loci, which may play a role in lymph node metastasis of oral SCC.To identify a candidate gene for the putative tumor suppressor gene near D13S273-D13S176 loci, which may play a role in lymph node metastasis, differential display method and representational difference analysis, using tumors with and without LOH at these loci. Two DNA fragments were isolated and confirmed the presence on chromosome 13q. Using these DNA fragments as probes, Northern blot analysis revealed the different expression levels between tumors with and without lymph node metastasis, suggesting that these fragments may be a part of putative tumor suppressor genes involved in metastasis.
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