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Genetic diagnosis of micrometastasis in oral cancer

Research Project

Project/Area Number 10470434
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionEhime University

Principal Investigator

HAMAKAWA Hiroyuki  Faculty of Medicine, Ehime University, professor, 医学部, 教授 (20127905)

Co-Investigator(Kenkyū-buntansha) TANIOKA Hiroaki  Faculty of Medicine, Ehime University, professor, 医学部, 教授 (10028748)
FUKUZUMI Masakuni  University Hospital, Ehime University, Instructor, 医学部・附属病院, 助手 (60294815)
KAYAHARA Hiroaki  University Hospital, Ehime University, Instructor, 医学部・附属病院, 助手 (50263942)
Project Period (FY) 1998 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥11,500,000 (Direct Cost: ¥11,500,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2000: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1998: ¥5,200,000 (Direct Cost: ¥5,200,000)
KeywordsOral cancer / Micrometastasis / Genetic diagnosis / Cervical lymph node / Circulating cancer cell / quantitative PCR / SCCA mRNA / Keratin 13 mRNA / 定量化PCR / SCCA / MASA法 / p53 / K13 / K20 / テロメラーゼ / K19 / SCC antigen
Research Abstract

Radical cure of primary oral squamous cell carcinoma (SCC) has improved owing to the development of diagnosis and treatment modalities, The 5-year survival rate of patients with multiple nodal metastases and/or distant metastasis is significantly low. Therefore, early detection of micrometastases in the cervical lymph node (LNs) and in the peripheral blood is expected to further improve their survival rate. We studied on several issueaas follows.
1. Microscopical detection of micrometastasis in the cervical lymph nodes of patients with oral cancer by means of semiserial sectionings.
2. Analysis of suitable tumor marker for detecting micometastasis of oral cancer cells.
3. Genetic diagnosis of micrometastasis in the cercical lymph nodes of clinical patients using conventional PCR and real time TaqMan PCR
4. Basic study for morphological detection of circulating cancer cells in the peripheral blood. -smear specimen, enrichment by cytospin, immunomagnetic separation by Dynabeads -
[Results]
1. … More Five-hundred fifty-four nonmetastatic cervical lymph nodes taken from 73 patients with oral cancer were subjected to hematoxylin-eosin (HE) staining and keratin immunohistochemistry (IHC). Micrometastases, defined as foci 【less than or equal】 3 mm, were detected in 29 sites of 23 lymph nodes (4.2%) of 16 patients (21.9%). Nine patients (12.3%) showed pN upgrading, i.e., 6 from PNO to pN1, 1 from PNO to pN2b, and 2 from pN1 to pN2b. The remaining 13 lymph 【less than or equal】 nodes with occult metastasis were involved in 5 pN2b and 2 pN2c patients resulting in no pN upgrading. The occult metastasis was also detected in six small lymph nodes 5 mm in diameter. The average minor axis of the micrometastasis was 1.36 ± 0.85mm. We propose that the lymph nodes should be cut and examined in 1-mm intervals to detect micrometastatic foci and to evaluate the accurate pN classification.
2. Keratin 13 and SCCA mRNAs are promising tumor marker for detecting micrometastasis of oral cancer by means of conventional RT-PCR.
3. We examined the expression of SCCA mRNA in 12 primary tumors and 212 cervical LNs (101 LNs taken from 8 patients with tongue cancer, 71 from 7 patients with gingival cancer, 19 from 2 patients with laryngeal cancer, 9 from 2 patients with pharyngeal cancer, 7 from 1 patient with cancer of the buccal mucosa, and 5 from 1 patient with cancer of floor of the mouth). Detectability of metastatic LNs by nested and single reverse transcriptase-polymerase chain reaction (RT-PCR) was compared with semiserial sections (hematoxylin-eosin staining and keratin immunostaining). All primary tumors expressed SCCA mRNA. Of 198 histologically metastasis-negative nodes, SCCA mRNA was detected in 37 (18.7%) by nested PCR. Eleven micrometastatic foci in 9 LNs (4.6%) were discovered by semiserial sectioning. This suggests that SCCA mRNA is a promising tumor marker for detecting the micrometastases in cervical LNs of head and neck cancer.
Real time quantitative PCR method was more sensitive than conventional PCR. One or several cacer cells are detectable by this method (unpublished data).
4. We contrived how to enrich whole mononuclear cells (MNCs) spiked with cancer cells on one slide specimen. Using this method and cytospin, cancer cells and MNCs from 1 ml of blood (without red blood cells) are easily checked on a 1.3 x 2.0 cm slide glass. This method allowed shortening of examination time. Next, we found that EOFR is a promising antibody for the immunomagnetic separation of oral cancer cells circulating in the peripheral blood and in the bone marrow. Less

Report

(5 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (69 results)

All Other

All Publications (69 results)

  • [Publications] Hamakawa H, Bao Y, Takarada M, Fukuzumi M, Tanioka H: "Cytokeratin Expression in Squamous Cell Carcinoma of the Lung and Oral Cavity : An immunocytochemical study with possible clinical relevance"Oral Surg Oral Med Oral Pathol. 85. 438-444 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sumida T, Sogawa K, Hamakawa H Sugita A, Tanioka H, Ueda N: "Detection of telomerase activity in oral lesions"J Oral Pathol Med.. 27. 111-115 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sogawa K, Yamada T, Muramatsu Y, Sumida T, Hamakawa H, Oda H, Miyake H, Tashiro S, Matsuda M, Matsumoto K, Okutani K: "Decrease of nuclear protein phosphatase 1 acitivityand induction of mitotic arrest and apoptosis by a marine microalgal polysaccharide in human myeloid leukemia U937 cells"Res Commun Mol Pathol Pharmacol. 99. 267-282 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sogawa K, Sumida T, Hamakawa H, Yamada T, Matsumoto K, Matsuda M, Oda H, Miyake H, Tashiro S, Okutani K: "Inhibitory effect of a marine microalgal polysaccharide on the telomerase activity in K562 cells"Res Commun Mol Pathol Pharmacol. 99. 259-265 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamada T, Sogawa K, Kin J-K, Izumi K, Suzuki Y, Muramats Y, Sumida T, Hamakawa H, Matsumoto K: "Increased polyploid, delayed mitosis and reduced protein phosphatase-1 activity associated with excess copper in Long Evans Cinnamon rat"Res Commun Mol Pathol Pharmacol. 99. 283-304 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Moralejo DH, Wei K, Wei S, Ogino T, Yamada T, Sogawa K, Hamakawa H, Szpirer C, Carbone C, Matsumoto K: "Mapping of the genes for rat P-glycoprotein 1,2,and 3 (Pgy-1, Pgy-2, and Pgy-3) to chromosome 4"Exp Anim. 47. 283-284 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hamakawa H, Sumida T, Tanioka H, Sogawa K, Yamada T: "Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis"Res Commun Mol Pathol Pharmacol. 101. 115-126 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sumida T, Hamakawa H, Sogawa K, Sugita A, Tanioka H, Ueda N: "Telomerase components as a diagnostic tool in human oral lesions"Int J Cancer. 80. 1-4 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hamakawa H, Bao Y, Tanioka H, Sogawa K, Yamada T: "Species specificity of cytokeratin polypeptide expression in the submandibular gland"Cell Moll Biol. 45. 265-276 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sumida T, Hamakawa H, Sogawa K, Bao Y, Zen H, Sugita A, Nezu K, Abe Y, Tanioka H, Ueda N: "Telomerase activation and cell proliferation during 9, 1O-dimethyl-1, 2-benzanthracene-induced hamster cheek pouch carcinogenesis"Mol Carcinogenesis. 25. 164-168 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hamakawa H, Fukuzumi M, Bao Y, Sumida T, Onishi A, Tanioka H, Sato H, Yumoto E: "Genetic diagnosis of micrometastasis based on SCC antigen mRNA in cervical lymph nodes of head and neck cancer"Clin Exp Metastasis. 17. 593-599 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sogawa K, Yamada T, Sumida T, Hamakawa H, Oda H, Tashiro S, Matsuda M, Matsumoto K, Okutani K: "Induction of K-562 cell apoptosis and inhibitory effect of DNA topoisomerase-I by a marine microalgal G3 polysaccharide"Life Science. 66. 227-231 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sumida T, Hamakawa H, Sogawa K, Tanioka H, Ueda N: "Clinical usefulness of telomerase assay for the detection of lymph node metastasis in patients with oral malignancy"Arch Pathol Lab Med. 124. 398-400 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hamakawa H, Takemura K, Sumida T, Kayahara H, Tanioka H, Sogawa K: "Histological study on pN upgrading of oral cancer"Virchows arch. 437. 116-121 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hamakawa H, Fukuzumi M, Bao Y, Sumida T, Kayahara H, Onishi A, Sogawa K: "Keratin mRNA for detecting micrometastases in cervical lymph nodes of oral cancer"Cancer Lett. 160. 115-123 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fukuzumi M, Hamakawa H, Onishi A, T Sumida, Tanioka H: "Gene expression of GLUT isoforms and VHL in oral squamous cell carcinoma"Cancer Lett. 161. 133-140 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T Sumida, H Hamakawa, K Otsuka, H Tanioka: "Leiomyosarcoma of the maxillary sinus with cervical lymph node metastasis"J Oral Maxillofac Surg. 59. 568-571 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T Sumida, H Hamakawa: "Telomerase and oral cancer"Oral Oncol. 37. 333-340 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kayahara H, Yamagata H, Miki T, Tanioka H, Hamakawa H: "Frequent loss of heterozygosity at 3p25-p26 is associated with invasive oral squamous cell carcinoma"J Human Genet. 46. 335-341 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ferlito A, Partridge M, Brennan JA, Hamakawa H: "Lymph node micrometastasis in head and neck cancer ; a review"Acta Otolaryngol. 121. 660-665 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mese H, Ueyama Y, Suzuki A, Nakayama S, Sasaki A, Hamakawa H, Matsumura T: "Inhibition of telomerase activity as a measure of tumor cell killing by cisplatin in squamous cell carcinoma cell line"Chemotherapy. 47. 136-142 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Begum NM, Nakashiro K, Kawamata H, Uchida D, Shintani S, Ikawa Y, Sato M, Hamakawa H: "Expression of peroxisome proliferator-activated receptor gamma and the growth inhibitory effect of its synthetic ligands in human salivary gland cancer cell lines"International Journal of Oncology. 20(in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Shintani, M.Mihara, Y.Nakahara, T.Aida, T.Tachikawa, H.Hamakawa: "Lymph node metastasis of oral cancer visualised in live tissue by green fluorescent protein expression"Oral Oncology. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hamakawa H., Bao Y., Takarada M., Fukuzumi M., Tanioka H.:: "Cytokeratin Expression in Squamous Cell Carcinoma of the Lung and Oral Cavity: An immunocytochemical study with possible clinical relevance"Oral Surg Oral Med Oral Pathol. 85. 438-44 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sumida T., Sogawa K., Hamakawa H., Sugita A., Tanioka H., Ueda N.: "Detection of telomerase activity in oral lesions"J Oral Pathol Med. 27. 111-5 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sogawa K., Yamada T., Muramatsu Y., Sumida T., Hamakawa H. Oda H., Miyake H., Tashiro S., Matsuda M., Matsumoto K., Okutani K.: "Decrease of nuclear protein phosphatase 1 activity and induction of mitotic arrest and apoptosis by a marine microalgal polysaccharide in human myeloid leukemia U937 cells"Res Commun Mol Pathol Pharmacol. 99. 267-282 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sogawa K., Sumida T., Hamakawa H., Yamada T., Matsumoto K., Matsuda M., Oda H., Miyake H., Tashiro S., Okutani K.: "Inhibitory effect of a marine microalgal polysaccharide on the telomerase activity in K562 cells"Res Commun Mol Pathol Pharmacol. 99. 259-265 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamada T., Sogawa K., Kin J.-K., Izumi K., Suzuki Y., Muramatsu Y., Sumida T., Hamakawa H., Matsumoto F.: "Increased polyploid, delayed mitosis and reduced protein phosphatase-1 activity associated with excess copper in Long Evans Cinnamon rat"Res Commun Mol Pathol Pharmacol. 99. 283-304 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Moralejo D.H., Wei K., Wei S., Ogino T., Yamada T., Sogawa K., Hamaawa H., Szpirer C., Carbone C., Matsumoto K.: "Mapping of the genes for rat P-glycoprotein 1,2,and 3(Pgy-1, Pgy-2, and Pgy-3) to chromosome 4"Exp Anim. 47. 283-284 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hamakawa H., Sumida T., Tanioka H., Sogawa K., Yamada T.: "Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis"Res Commun Mol Pathol Pharmacol. 101. 115-126 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sumida T., Hamakawa H., Sogawa K., Sugita A., Tanioka H., Ueda N.: "Telomerase components as a diagnostic tool in human oral lesions"Int J Cancer. 80. 1-4 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hamakawa H., Bao Y., Tanioka H., Sogawa K., Yamada T.: "Species specificity of cytokeratin polypeptide expression in the submandibular gland"Cell Moll Biol. 45. 265-276 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sumida T., Hamakawa H., Sogawa K., Bao Y., Zen H., Sugita A., Nezu K., Abe Y., Tanioka H., Ueda N.: "Telomerase activation and cell proliferation during 9, 10-dymethyl-1, 2-benzanthracene-induced hamster cheek pouch carcinogenesis"Mol Carcinogenesis. 25. 164-168 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hamakawa H., Fukuzumi M., Bao Y., Sumida T., Onishi A., Tanioka H., Sato H., Yumoto E.: "Genetic diagnosis of micrometastasis based on SCC antigen mRNA in cervical lymph nodes of head and neck cancer"Clin Exp Metastasis. 17. 593-599 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sogawa K., Yamada T., Sumida T., Hamakawa H., Oda H., Tashiro S., Matsuda M., Matsumoto K., Okutani K.: "Induction of K-562 cell apoptosis and inhibitory effect of DNA topoisomerase-I by a marine microalgal G3 polysaccharide"Life Science. 66. 227-231 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sumida T., Hamakawa H., Sogawa K., Tanioka H., Ueda N.: "Clinical usefulness of telemerase assay for the detection of lymph node metastasis in patients with oral malignancy"Arch Pathol Lab Med. 124. 398-400 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hamakawa H., Takemura K., Sumida T., Kayahara H., Tanioka H., Sogawa K.: "Histological study on pN upgrading of oral cancer"Virchows arch. 437. 116-121 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hamakawa H., Fukuzumi M., Bao Y., Sumida T., Kayahara H., Onishi A., Sogawa K.: "Keratin mRNA for detecting micrometastases in cervical lymph nodes of oral cancer"Cancer Lett. 160. 115-123 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fukuzumi M., Hamakawa H., Onishi A., T. Sumida, Tanioka H.: "Gene expression of GLUT isoform and VHL in oral squamous cell carcinoma"Cancer Lett. 161. 133-140 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Sumida, H. Hamakawa, K. Otsuka, H. Tanioka: "Leiomyosarcoma of the maxillary sinus with cervical lymph nod metastasis"J Oral Maxillofac Surg. 59. 568-571 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Sumida, H. Hamakawa: "Telomerase and oral cancer"Oral Oncol. 37. 333-340 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kayahara H., Yamagata H., Miki T., Tanioka H., Hamakawa H.: "Frequent loss of heterozygosity at 3p25-p26 is associated with invasive oral squamous cell carcinoma"J Human Genet. 46. 335-341 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ferlito A., Partridge M., Brennan J.A., Hamakawa H.: "Lymph node micrometastasis in head and neck cancer; a review"Acta Otolaryngol. 121. 660-665 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mese H., Ueyama Y., Suzuki A., Nakayama S., Sasaki A., Hamakawa H., Matsumura T.: "Inhibition of telomerase activity as a measure of tumor cell killing by cisplatin in squamous cell carcinoma cell line"Chemotherapy. 47. 136-142 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Begum N.M., Nakashiro K., Kawamata H., Uchida D., Shintani S., Ikawa Y., Sato M., Hamakawa H.: "Expression of peroxisome proliferator-activated receptor gamma and the growth inhibitory effect of its synthetic ligands in human salivary gland cancer cell lines"International Journal of Oncology. 20 (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Shintani, M. Mihara, Y. Nakahara, T. Aida, T. Tachikawa, H. Hamakawa: "Lymph node metastasis of oral cancer visualised in live tissue by green fluorescent protein expression"Oral Onchology. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sumida T: "Telomerase and oral cancer"Oral Oncol. 37. 333-340 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kayahara H: "Frequent loss of heterozygosity at 3p25-p26 is associated with invasive squamous cell carcinoma"J Human Genet. 46. 335-341 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ferlito A: "Lymph node micrometastasis in head and neck cancer ; a review"Acta Otolaryngol. 121. 660-665 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Begum NM: "Expression of peroxisome proliferator-activated receptor gamma and the growth inhihitory effect of its synthetic ligands in human salivary gland"Int J Oncol. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shintani S: "Lymph node metastasis of oral cancer visualised in live tissue by green fluorescent protein expression"Oral Oncol. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 浜川裕之: "口腔癌微小転移の遺伝子診断"日本口腔科学会雑誌. 50. 1-10 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sumida T: "Clinical usefulness of telomerase assay for the detection of lymph node metastasis in patients with oral malignancy."Arch Pathol Lap Med. 124. 398-400 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hamakawa H: "Histological study on pN upgrading of oral cancer."Virchows arch. 437. 116-121 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hamakawa H: "Kertin mRNA for detecting micrometastases in cervical lymph nodes of oral cancer."Cancer letters. 160. 115-123 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fukuzumi M: "Gene expression of GLUT isoforms and VHL in oral squamous cell carcinoma."Cancer letters. 161. 133-140 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sumida T: "Telomerase and oral cancer."Oral Oncol. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 浜川裕之: "口腔癌微小転移の遺伝子診断"日本口腔科学会雑誌. (印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] Hamakawa H: "Species Specificity of cytokeratin polypeptide expression in the submandibular gland"Cell Moll Biol. 45. 265-276 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 浜川裕之: "頭頸部腫瘍リンパ節微小転移の遺伝子診断"頭頸部腫瘍. 25(1). 81-86 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sumida T: "Telomerase activation and cell proliferation during 9,10-dimethyl-1,2-benzanthracene-induced hamster cheek pouch carcinogenesis"Mol Carcinogenesis. 25. 164-168 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sumida T: "Clinical usefulness of telomerase assay for the detection of lymph node metastasis in patients with oral malignancy"Arch Pathol Lab Med.. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hamakawa H: "Histological study on pN upgrading of oral cancer."Virchows arch. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hamakawa H: "Genetic diagnosis of micrometastasis based on SCC antigen mRNA in cervical lymph nodes of head and neck cancer"Clin Exp Metastasis. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Hamakawa: "Cytokeratin expression in squamous cell carcinoma of the lung and oral cavity. An immunohistochemical study with possible clinical relevance." Oral Surg Oral Med Oral Pathol. 85. 438-444 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Sumida: "Detection of telomerase activity in oral lesions." J Oral Pathol Med. 27. 111-115 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Hamakawa: "Extraction of cytokeratin from the human submandibular gland and electrophoretic analysys" Res Commun Mol Pathol Pharmacol. 101. 115-126 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Hamakawa: "Species specificity of cytokeratin polypeptide expression in the submandibular gland" Cell Moll Biol. 45in press. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 浜川裕之: "頭頸部腫瘍リンパ節微小転移の遺伝子診断" 頭頸部腫瘍. 25(1)印刷中. (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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