| Project/Area Number |
10470440
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Department of Oral Surgery, Sapporo Medical University School of Medicine |
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Principal Investigator |
NOBUYUKI Tanaka Sapporo Medical University, School of Medicine Assoc. Prof., 医学部, 助教授 (50163548)
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| Co-Investigator(Kenkyū-buntansha) |
ODAJIMA Tetsuyo Sapporo Medical University, School of Medicine Assoc. Prof., 医学部, 講師 (00177239)
YOSHIDA Kouichi Sapporo Medical University, School of Medicine Assoc. Prof., 医学部, 講師 (60117653)
AKIRA Yamaguchi Sapporo Medical University, School of Medicine, Instructor, 医学部, 助手 (10210353)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1998: ¥11,900,000 (Direct Cost: ¥11,900,000)
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| Keywords | Gene-technology / Oral cancer / p130 / Degree of malignancy / β-catenin / p53 / p16 |
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| Research Abstract |
Immunohistochemical investigation of 131 oral squamous cell carcinomas with p130 revealed that positive staining with p130 was found in many well-differentiated carcinomas and low-grade malignancy cases.
In oral malignant melanoma, p130 positively was recognized in the cases of which survival period was over 10 years and p53 positively was recognized in the cases of which survival period was below 1 year.
In oral leukoplakie which is precancerous lesion, positive staining with p130 and Rb was shown in the cases with low grade of dysplasia and positive staining with p53 was shown in the cases with high grade of dysplasia. It is suggested that immunohistochemical examination with p130, Rb and p53 is helpful for predicting the clinical course of oral leukoplakie.
β-catenin gene mutation was not recognized in oral squamous cell carcinoma cases and oral squamous cell carcinoma cell line. However, β-catenin protein was accumulated in the nuclei and the cytoplasm with the expression of EGFR which is related to tyrosin-phospholyration of β-catenin protein and it is thought that the examination of β-catenin gene is effective for analysis of degree of malignancy of oral cancer.
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