| Project/Area Number |
10470444
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Surgical dentistry
|
|---|
| Research Institution | MATSUMOTO DENTAL UNIVERSITY |
|---|
Principal Investigator |
UEMATSU Takashi MATSUMOTO DENTAL UNIVERSITY, FACULTY OF DENTISTRY, ASSOCIATE PROFESSOR, 歯学部, 助教授 (40203476)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TANAKA Hitoshl MATSUMOTO DENTAL UNIVERSITY, FACULTY OF DENTISTRY, ASSISTANT PROFESSOR, 歯学部, 助手 (50267788)
長谷川 貴史 松本歯科大学, 歯学部, 講師 (50237971)
|
|---|
| Project Period (FY) |
1998 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥11,700,000 (Direct Cost: ¥11,700,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥6,600,000 (Direct Cost: ¥6,600,000)
|
|---|
| Keywords | ジペチジルペプチダーゼ IV / CD26 / 口腔癌 / T細胞 / TGF-β1 / 免疫抑制 / マーカーエンザイム / ジペプチジルペプチダーゼIV / 活性化抑制 / dipeptidyl peptidase IV / Lympocyte culture / DPPIV activity / Flowcytometry |
|---|
| Research Abstract |
To clarify the cause of the decrease in serum dipeptidyl peptidase (DPP) IV activity in patients with oral squamous cell carcinoma, the influence of malignant cell-derived cytokines to the cell surface DPPIV (CD26 / DPP IV) expression in human T cells was analyzed.
Decrease in CD26/DPPIV expression of T cell extract and culture supernatant was found in the 8-30 kDa fraction of KBCM. KB cells produced cytokines, especially 25 kDa of mature TGF-β1 downregulated CD26/ DPPIV expression in T cells, which was responsible for decreased DPPIV activity in the cultured supernatant. The level of p27^<kip> expression in T cells was maintained in addition to T cell G1 arrest in the cultivation with KBCM and was abolished by neutralizing anti-TGF-β1 antibody. :
Mature TGF-β1 produced by KB cells down-regulates the CD26/DPPIV expression in T cells concomitant with the suppression of T cell growth by maintaining p27^<kip> expression, which leads to a cell cycle arrest in G1, supporting the concept that malignant cell-derived soluble factors inhibit cell proliferation, cell activation and immune function in T cells resulting in a decrease in the tumor marker, serum CD26/DPPIV activity of oral cancer patients.
|
