| Project/Area Number |
10470459
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Periodontal dentistry
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
KURIHARA Hidemi Hiroshima Univ., Faculty of Dentistry, Professor, 歯学部, 教授 (40161765)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SHIBA Hideki Hiroshima Univ., Faculty of Dentistry, Research Associate, 歯学部, 助手 (60260668)
YOSHINO Hiroshi Hiroshima Univ., Faculty of Dentistry, Research Associate, 歯学部, 助手 (50240338)
TAKEMOTO Toshinobu Hiroshima Univ., Faculty of Dentistry, Research Associate, 歯学部, 助手 (00236506)
HINO Takemune Hiroshima Univ., Faculty of Dentistry, Research Associate, 歯学部, 助手 (20274102)
|
|---|
| Project Period (FY) |
1998 – 1999
|
| Project Status |
Completed (Fiscal Year 1999)
|
| Budget Amount *help |
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1999: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥4,700,000 (Direct Cost: ¥4,700,000)
|
|---|
| Keywords | autoantibody / human gingival fibroblast / parvovirus B19 / Campylobactor rectus / HLA class 11 / genotyping / 早期発症型歯周炎 / 自己抗原 / Th1 / Th2 / Parvovirus B19 / Campylobacter reclus |
|---|
| Research Abstract |
In this project, we evaluate autoimmune mechanisms on the development of early-onset periodontitis. Firstly, the presence of autoantibodies against human gingival fibroblast (GF) and human periodontal ligament derived fibroblast (PLF) in patients with periodontitis was analyzed. Totally fifty-one patients with periodontitis and 12 healthy subjects were examined with Western blotting and ELISA. On Western blotting analyses, patients with early-onset periodontitis (EOP) frequently recognized auto-antigen in GF. Especially, antigens with 95 kDa and 108 kDa were clearly detected with serum IgG from EOP patients. Both antigens were also recognized with serum IgG from rabbits immunized Campylobactor rectus. Patients who have these autoantibodies showed high serum IgG titer against C rectus. Therefore, there may be molecular mimicry and cross reactivity between GF antigen and C. rectus antigen. Some viral infections were involved in autoirnmune disease, such as Rheumatoid arthritis. Then, the relationship between parvovirus B 19 (PVB 19) infection and autoantibody production was analyzed by ELISA. The ratio of PVB 19 infection in patients with periodontitis was higher than in healthy subjects. All patients who produce high level of autoanubody against GF or PLF were infected with PVB19. Immune response is known to be resincted by the major histocompatible complex (MHC, human leukocyte antigen (HLA) in human). Then, we further analyzed the HLA class 11 genotypes of patients with periodontitis, autoantibody production and PVB 19 infection. PCR-RFLP analyses were taken to detenuine HLA class II genotype. The frequencies of DQA1*0101, DQA1*0501, and DQB1*0503 in patients with periodontitis, autoantibody production and PVB 19 infection were higher in other patients and healthy subjects.
|
