| Project/Area Number |
10470492
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
医薬分子機能学
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
HASHIDA Mitsuru Kyoto University, Graduate Sch. Pharm. Sci., Professor, 薬学研究科, 教授 (20135594)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAKURA Yoshinobu Kyoto University, Graduate Sch. Pharm. Sci., Professor, 薬学研究科, 教授 (30171432)
西川 元也 京都大学, 薬学研究科, 助手 (40273437)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 2000: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1998: ¥8,600,000 (Direct Cost: ¥8,600,000)
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| Keywords | gene therapy / non-viral vectors / plasmid DNA / liver targeting / glycosylated liposomes / glycosylated macromolecules / membrane-fusogenic peptides / DDS / 体内動態 / 動態発現相関 / DNAプラスミド / カチオン性リポソーム / ポリカチオン / コレステロール / カオチン性リポソーム / ガラクトース受容体 / エンドサイトーシス |
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| Research Abstract |
In vivo gene therapy is increasingly attractive as one of advanced therapeutic methodologies against various diseases. For successful gene therapy, it is necessary to develop the carriers delivering gene to the target in a selective and effective manner. In this study, we analyzed relationship between pharmacokinetics and gene expression of plasmid DNA complexed with macromolecular and particulate carriers, and developed some strategies for controlling gene delivery using macromolecular and particulate carriers bearing specific ligands. In vitro gene transfection experiments found glycosylated cationic macromolecules and liposomes effective for specific delivery via sugar recognition mechanisms. The pharmacokinetics and in vivo gene transfection of plasmid DNA complexes were also investigated. When plasmid DNA complexed with glycosylated carriers was intraportally injected, it selectively accumulated and exhibited transfection activity in the liver. Membrane-fusogenic peptides were introduced to glycosylated cationic poly (amino acids), to control an intracellular trafficking of plasmid DNA complexes. The complexes were highly effective and liver-specific even when intravenously injected. Throughout this study, we demonstrated that in vivo expression of exogenous genes was much improved by controlling their pharmacokinetics, and developed the prototypes of gene delivery systems towards various diseases such as hepatic disorders.
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