• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Research on the role of angiotensinogen gene in the pathogenesis of essential hypertension

Research Project

Project/Area Number 10470504
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Human genetics
Research InstitutionASAHIKAWA MEDICAL COLLEGE

Principal Investigator

HATA Akira  ASAHIKAWA MEDICAL COLLEGE, PROFESSOR, 医学部, 教授 (00244541)

Co-Investigator(Kenkyū-buntansha) INOUE Ituro  THE INSTITUTE OF MEDICAL SCIENCE, THE UNIVERSITY OF TOKYO, ASSOCIATE PROFESSOR, 医科学研究所, 助教授 (00192500)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥12,200,000 (Direct Cost: ¥12,200,000)
Fiscal Year 1999: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1998: ¥7,300,000 (Direct Cost: ¥7,300,000)
Keywordsangiotensinogen / hypertension / salt / mouse / gene copy number / マウスモデル / 遺伝子発現 / 塩分摂取
Research Abstract

1) In vivo expression of angiotensinogen gene (AGT) is different between T allele and M allele. We have previously demonstrated about 20% higher expression in T allele than in M allele by way of in vitro experiments. In this study, we have tried to show allele dependent higher expression of AGT in vivo. For this purpose, we collected human cardiac tissue obtained in the operation of coronary artery bypass grafting (CABG). Out of 64 samples, 11 samples were revealed from the patient whose genotype is heterozygous of M235T (MT type). With these 11 samples, we have performed allele specific expression assay with primer pairs of P^<32> end-labeled common and allele specific oligonucleotide primer and Taq DNA ligase. Densitometrically, we found AGT expression from T alleles were 0 to 20% higher than that from M allele. This result may indicate the higher expression of AGT could be the cause of hypertension susceptibility.
2) We tried to show the difference of salt sensitivity in mice whose number of AGT gene copies ranged from 1 to 4. As the developer of these mice claimed, we have observed copy number dependent increase of blood pressure. However, we could not detect significant difference of blood pressure between high salt load (8%) and low salt diet (0.6%) in any combination of mice. Two possibilities could be considered for our result. One could be due to tail-cuff method we employed to measure blood pressure. The other could be due to the mouse strain which may be salt insensitive.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Oike Yet al.: "Truncated CBP protein leads to classical Rubinstein-Taybi syndrome phenotypes in mice: Implication of a dominant negative mechanism."Hum.Mol Genet. 8. 387-396 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Oike Y et al.: "Mice homozygous for a truncated form of CREB-biding protein exhibit defects in hematopoiesis and vasculo Oangiogenesis."Blood. 93. 2771-2779 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Saito T et al.: "Immunohistochemical determination of Wilson Copper-transporting P-type ATPase in the brain tissues of the rat"Neuroscience. 266. 13-16 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kamigaki M et al.: "Familial hypercholesterolemia with cholesteryl ester transfer protein deficiency"Int Med. 37. 523-527 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kobashi G: "Association of a variant of the angiotensinogen gene with pure type of hypertension in pregnancy in the Japanese"Am J Med Genet. 86. 232-236 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hirayama H et al.: "Five familial hypercholesterolemic kindreds in Japan with novel mutations of the LDL receptor gene"J Hum Genet. 43. 250-254 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Okabe M et al.: "Relationship between Cu metabolism hereditary disorders and distribution of Cu-metallothionein in kidneys.Metallothionein IV"Birkhauser verlag Basel/Switherland. 413-419 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 羽田 明: "高血圧.遺伝子治療開発ハンドブック"エヌ・ティー・エス. 92-95 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Oike Y et al.: "Truncated CBP protein leads to classical Rubinstein-Taybi syndrome phenotypes in mice : Implication of a dominant negative mechanism."Hum Mol Genet.. 8. 387-396 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Oike Y et al.: "Mice homozygous for a truncated form of CREB-binding protein exhibit defects in hemopoiesis and vasculoangiogenesis."Blood. 93. 2771-2779 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Saito T et al.: "Immunohistochemical determination of Wilson Copper-transporting P-type ATPase in the brain tissues of the rat."Neuroscience letters. 266. 13-16 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kamigaki M et al.: "Familial hypercholesterolemia with cholesteryl ester transfer protein deficiency."Int Med.. 37. 523-527 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kobashi G et al.: "Association of a variant of the angiotensinogen gene with pure type of hypertension in pregnancy in the Japanese."Am J Med Genet.. 86. 232-236 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hirayama H et al.: "Five familial hypercholesterolemic kindreds in Japan with novel mutations of the LDL receptor gene."J Hum Genet.. 43. 250-254 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Oike Y.et al.: "Truncated CBP protein leads to classical Rubinstein-Taybi syndrome phenotypes in mice : Implication of a dominant negative mechanism"Hum. Mol Genet. 8. 387-396 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Oike Y.et al.: "Mice homozygous for a truncated form of CREB-biding protein exhibit defects in hematopoiesis and vasculo angiogenesis"Blood. 93. 2771-2779 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Saito T.et al.: "Immunohistochemical determination of Wilson Copper-transporting P-type ATPase in the brain tissues of the rat"Neuroscience letters. 266. 13-16 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kamigaki M.et al.: "Familial hypercholesterolemia with cholesteryl ester transfer protein deficiency"Int Med. 37. 523-527 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hirayama H.et al.: "Five familial hypercholesterolemic kindreds in Japan with novel mutations of the LDL receptor gene"J Hum Genet. 43. 250-254 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Okabe, M.et al.: "Relationship between Cu metabolism hereditary disorders and distribution of Cu-metallothionein in kidneys. Metallothionein IV"Birkhauser verlag Basel/Switzerland. 413-419 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 羽田 明: "高血圧.遺伝子治療開発ハンドブック"エヌ・ティー・エス. 92-95 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Oike et al.: "Truncated CBP protein leads to classical Rubinstein-Taybi syndrome phenotypes in mice : Implication of a dominant negative mechanism." Hum.Mol.Genet,. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Oike et al.: "Failure of primitive haematopoiesis and vasculoangiogenesis in mice lacking transcriptional cofactor CBP" Hematology,. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kamigaki M et al.: "Familial hypercholesterolemia with cholesteryl ester transfer protein deficiency." Int.Med.37. 523-527 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hirayama H et al.: "Five familial hypercholesterolemic kindreds in Japan with novel mutations of the LDL receptor gene" J Hum Genet. 43. 250-254 (1998)

    • Related Report
      1998 Annual Research Report

URL: 

Published: 1998-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi