| Project/Area Number |
10470515
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SHIBATA Yoichi Dept. of Transfusion Medicine, The University of Tokyo, Professor, 医学部・附属病院, 教授 (30010474)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Koki Okinaka Memorial Center for Adult Disease Research, Researcher, 研究員 (50171484)
TSUNO Nelson Dept. of Transfusion Medicine, The University of Tokyo, Instructor, 医学部・附属病院, 助手 (50282637)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 1999: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1998: ¥7,400,000 (Direct Cost: ¥7,400,000)
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| Keywords | Pletelet Allo-antigen / Platelet Allo-antibody / Platelet Transfusion / Neonatal Alloimmune Thrombocytopenia / 血小板(HPA) / 新生児血小板減少症紫斑病 / 血小板輸血不応答 |
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| Research Abstract |
Platelet alloantibodies play a important role in neonatal alloimmune thrombocytopenia
HPA (human platelet antigens) are known to be involved in neonatal allo-immunethrombocytopenia (NAIT). For the detection of platelet antibodies, we developed MPHA (mixed passive hemagglutination) method for detecting anti-platelet antibodies.
Using MPHA test, we screened 20,333 sera from pregnant women (28-30 weeks gestation) and obtained the following results.
(1) Unexpectedly high prevalence of platelet antibodies in 198 sera (0.97%) The specificity of them were as follows,
anti-HPA-5b 168 sera(82, 3%), anti-HPA-4b 27 sera(13, 6%)
anti-HPA-6b 2 sera(1, 0%), anti-Nak-a 5 sera(2, 5%)
anti-HPA-4b+anti-HPA-5b 1 serum(0, 5%).
(2) Five cases of NAIT were due to anti-HPA-5b, two cases of NAIT were due to anti-HPA-4b, and one case was due to anti-HPA-4b and anti-HPA-5b. This is the first prospective study on neonatal alloimmune thrombocytemia.
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