• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Evaluation of Physical Exercise from Stress Protein (2)-Evallluation of Hypoactivity and Training Effect-

Research Project

Project/Area Number 10480004
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 体育学
Research InstitutionUniversity of Tokyo

Principal Investigator

ATOMI Yoriko  University of Tokyo, the Graduate School of Arts and Sciences, Department of Life Sciences, Professor, 大学院・総合文化研究科, 教授 (90125972)

Co-Investigator(Kenkyū-buntansha) MIZUNO Kazunori  University of Tokyo, the Graduate School of Arts and Sciences, Department of Life Sciences, Assistant Researcher, 大学院・総合文化研究科, 助手 (70251337)
HATTA Hideo  University of Tokyo, the Graduate School of Arts and Sciences, Department of Life Sciences, Assistant Professor, 大学院・総合文化研究科, 助教授 (60208535)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥11,600,000 (Direct Cost: ¥11,600,000)
Fiscal Year 1999: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1998: ¥9,900,000 (Direct Cost: ¥9,900,000)
Keywordsstress protein / running exercise / liver / skeletal muscle / HSP70 / hindlimb suspension model / muscle atrophy / GPT / 不活動 / αB-クリスタン / HSP72 / HSC73 / 後肢懸垂 / 運動
Research Abstract

In this study, 1) the effects of running exercise on the expression of stress protein, and 2) the effects of hindlimb suspension on the cytoskeleton tubulin/microtubule system which is the substrate for stress protein, were examined. 1) The stress protein expressions of HSP72 and HSC73, inducible and constitutive forms of HSP7Os, in exercise relating tissues (skeletal muscles and heart) and exercise non-relating tissues after two levels of exercises of low (speed : 13 m/min ; blood lactate<4 mM : LIE) and high (24 m/min ; blood lactate > 4 mM : HIE) intensities of running exercise were measured by Western blotting using anti-HSP72 and HSC73 antibodies. Serum GTP was measured as an index of liver damage after exercise. The HSP expressions after heat shock were measured as positive controls. Both the GPT levels and inducible HSP72 (3〜4 times) increased after HIE, as well as with small increases of HSC73 (20%), while only HSC73 increase was observed after LIE.Both HSP70s depressed after both exercises in hearts. 2) Tubulin content in the 5-day-suspended soleus muscles was significantly and specifically decreased, while that in the stretched muscles increased 3 times at 5 days and over 3 times at the 10 days of suspension. αB-crysallin content in precipitate fraction of muscle protein increased in suspended soleus muscles, suggesting that the association of αB-crystallin with denatured proteins in precipitate fraction increased in suspended muscle. From these results, habitual physical activity and low intensity of exercise express constitutive expressed form of HSP70 (HSC73) and higher intensity of exercise expresses inducible form of HSP70 (HSP72).

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Atomi,Y. et al.: "Fiber-type-specific alpha B-crystallin distribution and its shifts with T3 and PTU treatments in rat hindlimb muscles."J.Appl.Physiol.. 88. 1355-1364 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Atomi,Y. et al.: "Mechanism of Adaptability for Gravity from analyzed Stress Response of Cells : Visualization of dynamics of cultured cells and cytoskeleton utilizing GFP-fusion protein."Space Utilization Research. 17. 182-185 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohto,E. et al.: "Domain analysis of alpha B-crystallin for chaperone-like activity : oligomerization and substrate specificity."Molecular Biology of the Cell. 11. 119a (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 村上小百合 et al.: "機械的刺激を受けた筋芽細胞の動的応答"日本機械学会論文集. 66. 1432-1438 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Harimoto,M. et al.: "Maintenance of a round cell shape and physiological reponsiveness to Insulin of mature adipocytes by culturing in type I collagen gel."Connective Tissue. 32. 11-15 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Atomi,Y.: "Creation of Generality and Originality of Physical Education and Sport Science through Understanding of Body and Mind from Life Science."InXXX (調査中). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Atomi,Y.: "Why active life is important for health and longevity?-From thestudies of stress protein and mechanical stress-."In Current Reviews of Medical Science by Women. 150-153 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Atomi,Y. et al.: "Sense and antisense alpha B-crystallin expression in L6 myoblasts and C6 glioma cells results in different cell motility dependent on MT/Actin stability."Mol.Biol.Cell.. 10. 381A (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tanaka,M. et al.: "Cellular characteristics of alpha B-cystallin overexpress/suppressed myoblasts and its cytoskeletal relevance."Advances in Exercise and Sports Physiology. 4. 117 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Harimoto,M. et al.: "In vitro culture system of rat mature adipocytes which may reflect in vivo adipose tissue."Cell Struct.Funct.. 23. 127 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nagao,Y. et al.: "Effect of different type of mitochondrial DNA on preimplantation embryonic development in the mouse."J.Reprod.Dev.. 44. 129-134 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nagao,Y. et al.: "Decreased physical performance of congenic mice with mismatch between the nuclear and the mitochondrial genome"Genes Genet.Syst.. 73. 21-27 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Atomi,Y. et al.: "Sense and antisense modification of alpha B-crystallin production results In alterations on MT stability in glial and myoblast cells."Mol.Biol.Cell.. 9. 415A (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 跡見順子: "連載 生命科学から見るスポーツ「ストレッチは、なぜよいか」月刊スポーツメディスン28号"(有)ブックハウス・エイチディ. 5(48) (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 跡見順子: "新連載 生命科学から見るスポーツ「なぜ、生命科学を知ったほうがよいか」Sportsmedicine 2000 No.27"(有)ブックハウス・エイチディ. 5(48) (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 跡見順子 et al.: "身体運動・栄養・健康の生命環境科学 Q&A シリーズ「骨格筋と運動」"杏林書院. 128(164) (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 東京大学身体運動科学研究室 編: "教養としてのスポーツ・身体運動"東京大学出版会. 233 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 跡見順子: "生命科学的な身体・心身の理解から体育・スポーツ科学の普遍性と独自性を。「21世紀と体育・スポーツ科学の発展-日本体育学会第50回記念大会誌」"杏林書院. (8)279 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Atomi, Y.et al.: "Fiber-type-specific alpha B-crystallin distribution and its shifts with T3 and PTU treatments in rat hindlimb muscles."J.Appl.Physiol.. 66. 1355-1364 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Atomi, Y.et al.: "Mechanism of Adaptability for Gravity from analyzed Stress Response of Cells : Visualization of dynamics of cultured cells and cytoskeleton utilizing GFP-fusion protein."Space Utilization Research. 17. 182-185 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohto, E.et al.: "Domain analysis of alpha B-crystallin for chaperone-like activity : oligomerization and substrate specificity."Molecular Biology of the Cell. 11. 119a (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Murakami, S et al.: "Dynamic Response of Mechanical Stimulation in Myobrasts."Jap.Mechanics Ass.J. 66. 1432-1438 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Harimoto, M.et al.: "Maintenance of a round cell shape and physiological responsiveness to Insulin of mature adipocytes by culturing in type1 collagen gel."Connective Tissue. 32. 11-15 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Atomi, Y.et al.: "Sense and antisense alpha B-crystallin expression in L6 myoblasts and C6 glioma cells results in different cell motility dependent on MT/Actin stability."Mol.Biol.Cell.. 10. 381A (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tanaka, M.et al.: "Cellular characteristics of alpha B-cystallin overexpress/suppressed myoblasts and its cytoskeletal relevance."Advances in Exercise and Sports Physiology. 4. 117 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nagao, Y.et al.: "Effect of different type of mitochondrial DNA on preimplantation embryonic development in the mouse."J.Reprod.Dev.. 44. 129-134 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nagao, Y.et al.: "Decreased physical performance of congenic mice with mismatch between the nuclear and the mitochondrial genome"Genes Genet.Syst. 73. 21-27 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Harimoto, M.et al.: "In vitro culture system of rat mature adipocytes which may reflect in vivo adipose tissue."Cell Struct.Funct. 23. 127 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Atomi,Y.Toro,K.Matsuda,T.and Hatta,H.: "Fiver-type specific αB-ccrystallin distribution and its shifts with T3 and PTU treatments in rat hindlimb muscles"J.Appl.Physiol.. 88 (In press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Harimoto,M.Hirose.M.Hayashi,and Atomi,Y.: "Maintenance of a round cell shape and physiological responsiveness to Insulin of mature adipocytes by cultureing in type I collagen gel"Connective Tissue. 32 (In press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Atomi,Y. and Tanaka,M.: "Sense and antisense αB-crystallin expression in L6 myoblasts and C6 glioma cells results in defferent cell motility dependent on MT/Actin stability"Mol.Biol.Cell.. 10. 380A (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 伏木亭、跡見順子、大野秀樹編著: "身体運動・栄養・健康の生命環境科学Q&Aシリーズ 「栄養と運動」"杏林書院. 145 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y.Atomi: "In Current Reviews of Medical Science by Women"国際女性技術者・化学者会議 衣料部門分科会. 229 (1999)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1998-04-01   Modified: 2021-10-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi