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Study of production mechanism of DNA clustered damage in aqueous solution

Research Project

Project/Area Number 10480137
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 環境影響評価(含放射線生物学)
Research InstitutionHigh Energy Accelerator Research Organization

Principal Investigator

KOBAYASHI Katsumi  Institute of Materials Structure Science, High Energy Accelerator Research Organization, Associate Professor, 物質構造科学研究所, 助教授 (20114077)

Co-Investigator(Kenkyū-buntansha) TAKAKURA Kaoru  International Christian Univ., Physics dept, Associate Professor, 教養, 助教授 (80052281)
USAMI Noriko  Institute of Materials Structure Science, High Energy Accelerator Research Organization, Research Associate, 物質構造科学研究所, 助手 (60232807)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1998: ¥5,400,000 (Direct Cost: ¥5,400,000)
Keywordssynchrotron radiation / DNA strand breaks / monochromatic photons / plasmid DNA / scavenger / スカべンジャー
Research Abstract

Purpose of the study is to test our hypothesis that double strand breaks (DSB) of DNA, as a type of clustered damage, are mainly produced in a transiently-existing area (hot area) in which radicals are produced with high density by many energy deposition events in localized region. By changing the energy of monochromatic photons irradiated to aqueous samples, we can control the dimension of "hot area! Produced by the secondary photoelectron produced in water. We already observed that efficiency of DSB production become larger with lower energy photon, while single strand break (SSB) exhibit opposite dependence.
During the term of the study, we have observed that the X-ray photon energy dependence of strand bread induction in the presence of high concentration of radical scavenger is the same with the case of the solution without scavenger. This seems to suggest that our hypothesis is effective in intracellular environment. In order to examine the production efficiency of double strand breaks in living cells, we have established a method to determine the number of double strand break in the cellular DNA without any treatment with restrictive digestion enzyme, by utilizing pulse field gel electrophoresis. We will soon have the answer of the test of our hypothesis.
We have also investigated the production of DNA strand breaks by monochromatic vacuum UV photons (150 - 180 nm) in aqueous solution. We have found that VUV photon energy dependence of the production of DSB and SSB is different, indicating that production mechanism for the production of both type of strand breakage is different.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 高倉かほる: "放射光による生体試料の解析-DNA放射線損傷の研究"Radioisotope,. 47. 872-882 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 小林克己: "DNA損傷ができるまでに何が起こるか?-放射光を用いたDNA損傷生成過程に関する研究-"Radioisotope,. 48. 648-655 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Kobayashi: "Chemical reactions in aqueous systems in relation to radiation biology"Proceeding of 11th International Congress of Radiation Research.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] C. Le Sech: "Stand break induction by photoabsorption In DNA-bound molecules"Radiation Research. (印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] C. Le Sech: "Enhanced strand break induction of intercalated DNA by resonant metal-inner-shell photoabsorption"Canadian Journal of Physiology and Pharmacology. (印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kaoru TAKAKURA: "Analysis of Biological Samples Using Synchrotron Radiation-Research on Radiation damage to DNA Using Synchrotron Radiation"Radioisotope. 47. 872-882 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Katsumi KOBAYASHI: "Research on the Production Mechanism of DNA damages Using Synchrotron Radiation"Radioisotope. 48. 648-655 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Katsumi KOBAYASHI: "Chemical reactions in aqueous systems in relation to radiation biology"Proceedings of 11th International Congress of Radiation Research. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Claude LE SECH: "Strand break induction by photoabsorption in DNA-bound molecules"Radiation Research. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Claude LE SECH: "Enhanced strand break induction of intercalated DNA by resonant metal-inner-shell photoabsorption."Canadian Journal of Physiology and Pharmacology. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 小林克己: "DNA損傷ができるまでに何が起きるか? -放射光を用いたDNA損傷生成過程に関する研究-"Radioisotope. 48. 648-655 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K. Kobayashi: "Chemical reactions in aqueous systems in relation to radiation biology"Proceedings of 11th International Congress of Radiation Research. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] C. Le Sech: "Strand break induction by photoabsorption In DNA-bound molecules"Radiation Research. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] C. Le Sech: "Enhanced strand break induction of intercalated DNA by resonant metal-inner-shell photoabsorption"Canadian Journal of Physiology and Pharmacology. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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