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Mechanism of regulation of selective intracellular protein sorting by the family of Adaptor (-like) protein complexes

Research Project

Project/Area Number 10480155
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionKanazawa University (1999)
Chiba University (1998)

Principal Investigator

OHNO Hiroshi  Kanazawa University, Cancer Research Institute, Professor, がん研究所, 教授 (50233226)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥12,900,000 (Direct Cost: ¥12,900,000)
Fiscal Year 1999: ¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1998: ¥6,800,000 (Direct Cost: ¥6,800,000)
Keywordsclathrin / AP complexes / tyrosine-based sorting signal / sorting signals / selective sorting / endocytosis / epithelial cells / cell polarity / 細胞内蛋白輸送 / チロシン・シグナル / リソソーム / トランスゴルジ網
Research Abstract

Selective sorting of membrane proteins in the post-Golgi secretory and endocytic pathways are largely regulated by sorting signals encoded within the cytoplasmic tails of sorted proteins. One of the sorting signals, the tyrosine-based sorting signal, works for selective intake of the sorted molecules into the clathrin-coated vesicles (CCV), by interacting with the μ subunits of AP complexes, a coat component of the CCV. AP complexes consist a protein family, and the tyrosine-based sorting signals are known to be involved in many intracellular sorting pathways including endocytosis and basolateral sorting. This raised the possibility that each μ chain specifically recognizes a subset of the tyrosine-based sorting signals to produce specificity and diversity of the sorting. We utilized a yeast 2-hybrid assay to show this is the case.
We cloned a new μ homologue, μ1B, which is highly homologous to one of the ubiquitously expressed μ chains, μ1A. In contrast to μ1A, however, the expression of μ1B is restricted to epithelial cells. Plasma membrane of epithelial cells are physically divided into two domains, apical and basolateral, and membrane proteins are sorted selectively to these two domains. By reconstituting the expression of μ1B into an epithelial cell line lacking the μ1B expression, we were able to show that μ1B is involved in the selective sorting of membrane proteins to the basolateral plasma membrane in epithelial cells.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Ohno, H., Poy G., Bonifacino, J.S.: "Cloning of the gene encoding the murine clathrin-associated adaptor medium chain μ2 : gene organization, alternative splicing and chromosomal assignment"Gene. 210. 187-193 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohno, H., Aguilar, R. C., Yeh, D., Taura, D., Saito, T., Bonifacino, J. S.: "The Medium Subunits of Clathrin Adaptor Complexes Recognize Distinct but Overlapping Sets of Tyrosine-based Sorting Signals"J. Biol. Chem.. 273. 25915-25912 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohno, H., Tomemori, T., Nakatsu, F., Okazaki, Y., Aguilar, R. C., Foelsch, H., Mellman, I., Saito, T., Shirasawa, T., Bonifacino, J. S.: "μ1B : a novel adaptor medium chain expressed in polarized epithelial cells"FEBS Lett.. 449. 215-220 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Folsch, H., Ohno, H., Bonifacino, J. S., Mellman, I.: "A novel clathrin adaptor complex mediates basolateral targeting in polarized epithelial cells"Cell. 99. 189-198 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakatsu, F., Kadohira, T., Gilbert, D. J., Jenkins, N. A., Kakuda, H., Copeland, N. G., Saito, T., Ohno, H.: "Genomic structures and chromosomal mapping of the genes encoding clathrin-associated adaptor medium chains μ1A (Ap1m1) and μ1B (Ap1m2)"Cytogenet. Cell Genet.. 87. 53-58 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohno, H., Poy G., Bonifacino, J. S.: "Cloning of the gene encoding the murine clathrin-associated adaptor medium chain μ2 : gene organization, alternative splicing and chromosomal assignment."Gene. 210. 187-193 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohno, H., Aguilar, R. C., Yeh, D., Taura, D., Saito, T., Bonifacino, J. S.: "The Medium Subunits of Clathrin Adaptor Complexes Recognize Distinct but Overlapping Sets of Tyrosine-based Sorting Signals."J. Biol. Chem.. 273. 25915-25921 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohno, H., Tomemori, T., Nakatsu, F., Okazaki, Y., Aguilar, R. C., Foelsch, H., Mellman, I., Saito, T., Shirasawa, T.: "Bonifacino, J. S. μ1B : a novel adaptor medium chain expressed in polarized epithelial cells."FEBS Lett.. 449. 215-220 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Folsch, H., Ohno, H., Bonifacino, J. S., Mellman, I.: "A novel clathrin adaptor complex mediates basolateral targeting in polarized epithelial cells."Cell. 99. 189-198 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakatsu, F., Kadohira, T., Gilbert, D. J., Jenkins, N. A., Kakuda, H., Copeland, N. G., Saito, T., Ohno, H.: "Genomic structure and chromosomal mapping of the genes encoding clathrin-associated adaptor medium chains μ1A (Ap1m1) and μ1B (Ap1m2)."Cytogenet. Cell Genet.. 87. 53-58 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohno, H., Poy, G., Bonifacino, J.S.: "Cloning of the gene encoding the murine clathrin-associated adaptor medium chain μ2: gene organization, alternative splicing and chromosomal assignment"Gene. 210. 187-193 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ohno, H., Aguilar, R.C., Yeh, D., Taura, D., Saito, T., Bonifacino, J.S.: "The Medium Subunits of Clathrin Adaptor Complexes Recognize Distinct but Overlapping Sets of Tyrosine-based Sorting Signals."J. Biol. Chem. 273. 25915-25921 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ohno, H., Tomemori, T., Nakatsu, F., Okazaki, Y., Aguilar, R.C., Foelsch, H., Mellman, I., Saito, T., Shirasawa, T., Bonifacino, J.S.: "μ1B: a novel adaptor medium chain expressed in polarized epithelial cells."FEBS Lett.. 449. 215-220 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Folsch, H., Ohno, H., Bonifacino, J.S., Mellman, I.: "A novel coathrin adaptor complex mediates basolateral targeting in polarized epithelial cells."Cell. 99. 189-198 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nakatsu, F., Kadohira, T., Gilbert, D.J., Jenkins, N.A., Kakuda, H., Copeland, N.G., Saito, T., Ohno, H.: "Genomic structure and chromosomal mapping of the genes encoding clathrin-associated adaptor medium chains μ1A (Ap1m2) and μ1B (Ap1m2)"Cytogenet. Cell Genet.. 87. 53-58 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ohno,H.: "Cloning of the gene encoding the murine clathrin-associated adaptor medium chain μ2:gene organization,alternative splicing and chromosomal assignment." Gene. 210. 187-193 (1988)

    • Related Report
      1998 Annual Research Report
  • [Publications] Park,S.Y.: "Resistance of Fc receptor-deficient mice to fatal glomerulonephritis." J.Clin.Inv.102. 1229-1238 (1988)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ohno,H.: "The medium subunits of clathrin adaptor complexes recognize distinct but overlapping sets of tyrosine-based sorting signals." J.Biol.Chem.273. 25915-25921 (1988)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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