| Project/Area Number |
10480208
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
TAKADA Shinji Kyoto University, Graduate School of Science, Professor, 大学院・理学研究科, 助教授 (60206753)
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| Project Period (FY) |
1998 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥10,700,000 (Direct Cost: ¥10,700,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1999: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥3,500,000 (Direct Cost: ¥3,500,000)
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| Keywords | development / spinal cord / mouse / pattern formation / cell-cell interaction / Wnt / 形態形成 / 神経管 / 体節 / シグナル分子 |
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| Research Abstract |
The spinal cord is derived from the neural plate, an epithelial sheet overlying the notochord and flanked by epidermal ectoderm. The neuroepithelial cells are highly proliferative and finally differentiate into the numerous types of neurons including the motor neuron and interneurons. In the developing spinal cord, signals from the roof plate are required for the development of three classes of dorsal interneuron ; D1,D2 and D3, listed from dorsal to ventral. I demonstrate that absence of Wnt1 and Wnt3a, normally expressed in the roof plate, leads to diminished development of D1 and D2 neurons and a compensatory increase in D3 neuron populations. This occurs without significantly altered expression of BMP and related genes in the roof plate. Moreover, Wnt3a protein induces expression of D1 and D2 markers in the isolated medial region of chick neural plate. Thus, Wnt signaling plays a critical role in the specification of cell types for dorsal interneurons.
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