| Project/Area Number |
10480212
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
YASUHARA Osamu Molecular Neuroscience Research Center, Shiga University of Medical Science Associate Professor, 分子神経科学研究センター, 助教授 (80239772)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Hiroshi Molecular Neuroscience Research Center, Shiga University of Medical Science Professor, 分子神経科学研究センター, 教授 (40079736)
TOOYAMA Ikuo Molecular Neuroscience Research Center, Shiga University of Medical Science Professor, 分子神経科学研究センター, 教授 (20207533)
AIMI Yoshinari Molecular Neuroscience Research Center, Shiga University of Medical Science Reserch Associate, 分子神経科学研究センター, 助手 (20231756)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥12,100,000 (Direct Cost: ¥12,100,000)
Fiscal Year 2000: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 1998: ¥4,800,000 (Direct Cost: ¥4,800,000)
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| Keywords | alternative splicing / choline acetyltransferase / cholinergic neurons / retinal ganglion cells / antisense oligonucleotide / immunohistochemistry / parasympathetic nervous system / RT-PCR / 脳機能解析 / mRNA / ホスホロチオエート・オリゴ / 分子変換 / MAP2 |
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| Research Abstract |
Acetylcholine is a neurotransmitter in the central and peripheral nervous systems. Cholinergic neurons have been demonstrated by immunohistochemistry using antibodies to choline acetyltransferase (ChAT). Although most of such antibodies can clearly stain central cholinergic neurrons, they generally fail to demonstrate peripheral cholinergic neurons. We have recently found that peripheral cholinergic cells preferentially express an alternative splice variant of ChAT mRNA (pChAT mRNA). In the present research project, we have reevaluated both the central and peripheral cholinergic nervous system using the antibody to pChAT.The pChAT antibody stained peripheral parasympathetic neurons. In addition, certain populations of neurons in the central and peripheral nervous systems, which have never been regarded as a part of the cholinergic system, have been recognized to be pChAT-positive. They include primary sensory neurons in the dorsal root ganglia, retinal ganglion cells, and neurons in the tubelomammillary hypothalamic nucleus. We have also confirmed the expression of pChAT mRNA in such areas. The discovery of pChAT should facilitate investigations on the periphearl, as well as central, cholinergic systems.
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