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Cloning and functional analysis of taste receptors for sourness and saltiness

Research Project

Project/Area Number 10480220
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionNagoya City University

Principal Investigator

SHIMADA Shoichi  Nagoya City University, Medical School, Professor, 医学部, 教授 (20216063)

Co-Investigator(Kenkyū-buntansha) UEDA Takashi  Nagoya City University, Medical School, Research Associate, 医学部, 助手 (90244540)
HIRABAYASHI Yoshifumi  Nagoya City University, Medical School, Assistant Professor, 医学部, 講師 (30181184)
FUJIMORI Osamu  Nagoya City University, Medical School, Associate Professor, 医学部, 助教授 (30128350)
UGAWA Shinya  Nagoya City University, Medical School, Research Associate, 医学部, 助手 (20326135)
植田 弘美  名古屋市立大学, 医学部, 助手 (30213359)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥10,300,000 (Direct Cost: ¥10,300,000)
Fiscal Year 2000: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1999: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥5,000,000 (Direct Cost: ¥5,000,000)
KeywordsSour Receptor / Taste sensation / Receptor / Cloning / Gene / Adaptation
Research Abstract

We have identifieda cDNA encoding a taste receptor for sourness (MDEG1) from a rat circumvallate papilla cDNA library by combined cDNA homology and functional expression approaches. When expressed in Xenopus laevis oocytes, MDEG1 showed characteristics of a proton-gated amiloride-sensitive cation channel. Interestingly, acidic stimulation by acetic acid, the chief ingredient of vinegar, generatedlarger inwardcurrents than that by hydrochloric acidat equal pH.In situ hybridization revealed that the mRNA of MDEG1 was exclusively localized in taste buds. MDEG1 immunoreactivity was found on the cell surface and in the apical portion of taste bud cells, especially in type III cells which are classifiedas gustatory receptor cells. This is the first taste receptor cDNA to be cloned. To isolate another subunit of the sour-taste receptor, we screened a rat circumvallate papilla cDNA library and iden tified MDEG2. Immunohistochemical and electrophysiological studies demonstrated that MDEG2 formed heterooligomeric channels with MDIEG1 at the apical portion of single taste-cells and modified the desensitization of MDEG1 channel. The desensitization rate of the heterooligomer was dramatically altered by a single amino acid substitution of Gly481 in MDEG2 without changing other channel properties. These findings suggest that MDEG2 regulates the sour-taste receptor desensitization contributing to sour-taste adaptation and that Gly481 in MDEG2 is the key amino acidresidue that controls the duration of sourtaste sensation.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (49 results)

All Other

All Publications (49 results)

  • [Publications] Ugawa S.: "Cloning of a mouse colonic system B 0+amino acid transporter related to amino acid absorption in the colon."Am J Physiol. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ueda T: "Diffuse brain injury induces local expression of Na(+)/myo-inositol cotransporter in the rat brain."Mol Brain Res. 86. 63-69 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Horimoto M: "A novel strategy for cancer therapy by mutated mammalian degenerin gene transfer"Cancer Gene Ther. 7. 1341-1347 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Saishin Y: "Retinal fascin : functional nature, subcellular distribution, and chromosomal localization."Invest Ophthalmol Vis Sci. 41. 2087-2095 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ozeki H: "Apoptosis is associated with formation and persistence of the embryonic fissure."Cur Eye Res. 20. 367-372 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ugawa S: "Isolation of a gene for a sour-taste receptor from a rat circumvallate papilla cDNA library."Jpn.J.Taste Smell Res. 7. 177-182 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sakata K.: "Cloning of a bovine orphan transporter and its short splicing variant"FEBS Lett. 443. 267-270 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mizuno N.: "Molecular cloning and characterization of rat trp homologues from brain."Mol Brain Res. 64. 41-51 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nishimura M: "Expression of norepinephrine and serotonin transporter mRNAs in the rat superior cervical ganglion."Mol Brain Res. 67. 82-86 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ito Y.: "Comparison between the decrease of dopamine transporter and that of L-DOPA uptake for detection of early to advanced stage of Parkinson's disease in animal models."Synapse. 31. 178-185 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 島田昌一: "味覚受容体遺伝子"脳21. 2. 304-308 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Okada T.: "Tetrahydropapaveroline and its derivatives inhibit dopamine uptake through dopamine transporter expressed in HEK293 cells."Neurosci Res. 30. 87-90 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ugawa S.: "Receptor that leaves a sour taste in the mouth."Nature. 395. 555-556 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nishimura M.: "MK-801 blocks monoamine transporters expressed in HEK cells."FEBS Lett. 423. 376-380 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Okada T.: "Assessment of affinities of beta-CIT, beta-CIT-FE, and beta-CIT-FP for monoamine transporters permanently expressed in cell lines."Nucl Med Biol. 25. 53-58 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nonaka M.: "Increased transcription of glutamate-aspartate transporter (GLAST/GluT-1) mRNA following kainic acid-Induced limbic seizure."Mol Brain Res. 55. 54-60 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kitamura H.: "Inhibition of myo-inositol transport causes acute renal failure with selective medullary injury in the rat."Kidney Int. 53. 146-153 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ugawa S.: "Cloning of mouse colonic system B O_+ amino acid transporter related to amino acid absorption in the colon."Am J Physiol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ueda T.: "Diffuse brain injury induces local expression of Na (+) / myo- inositol cotran sporter in the rat brain."Mol Brain Res.. 86. 63-69 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Horimoto M.: "A novel strategy for cancer therapy by mutated mammalian degenerin gene transfer."Cancer Gene Ther. 7. 1341-1347 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Saishin Y.: "Retinal fascin : functional nature, subcellular distribution, and chromosomal localization."Invest Ophthalmol Vis Sci. 41. 2087-2095 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ozeki H.: "Apoptosis is associated with formation and persistence of the embryonic fissure."Cur Eye Res. 20. 367-372 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ugawa S.: "Isolation of a gene for a sour-taste receptor from a rat circumvallate papilla cDNA library."Jpn.J.Taste Smell Res. 7. 177-182 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sakata K.: "Cloning of a bovine orphan transporter and its short splicing variant"FEBS Lett. 443. 267-270 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mizuno N.: "Molecular cloning and characterization of rat trp homologues from brain."Mol Brain Res. 64. 41-51 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nishimura M.: "Expression of norepinephrine and serotonin transporter mRNAs ntherat superior cerical ganglion."Mol Brain Res. 67. 82-86 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ito Y.: "Comparsion between the decrease of dopamine transporter and that of L-DOPA uptake for detection of early to advanced stage of Parkinson's disease in animal models."Synapse. 31. 178-185 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ckada T.: "Tetrahydropapaveroline and its derivatives inhibit dopamine uptake through dopamine transporter expressed in HEK 293 cells."Neurosci Res. 30. 87-90 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ugawa S.: "Receptor that leaves a sour taste in the mouth."Nature. 395. 555-556 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ckada T.: "Assessment of affinities of beta-CIT, beta-CIT-FE, and beta-CIT-FP for monoamine transporters permanently expressed in cell lines."Nucl Med Biol. 2. 53-58 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nonaka M.: "Increased transcription of glutamate-aspartate transporter (GLAST/GluT-1) mRNA following kainic acid- induced limbic seizure."Mol Brain Res. 55. 54-60 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kitamura H.: "Inhibition of myo-inositiol transport causes acute renal failure with selective medullary injury in the rat."Kidney Int. 5. 146-153 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ugawa S.: "Cloning of a mouse colonic system B 0+ amino acid transporter related to amino acid absorption in the colon."Am J Physiol. (印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] Ueda T: "Diffuse brain injury induces local expression of Na(+)/myo-inositol cotransporter in the rat brain."Mol Brain Res. 86. 63-69 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Horimoto M: "A novel strategy for cancer therapy by mutated mammalian degenerin gene transfer."Cancer Gene Ther. 7. 1341-1347 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Saishin Y: "Retinal fascin : functional nature, subcellular distribution, and chromosomal localization."Invest Ophthalmol Vis Sci. 41. 2087-2095 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ozeki H: "Apoptosis is associated with formation and persistence of the embryonic fissure."Cur Eye Res. 20. 367-372 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ugawa S: "Isolation of a gene for a sour-taste receptor from a rat circumvallate papilla cDNA library."Jpn.J.Taste Smell Res. 7. 177-182 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sakata K.: "Cloning of a bovine orphan transporter and its short splicing variant"FEBS Lett. 443. 267-270 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mizuno N.: "Molecular cloning and characterization of rat trp homologues from brain"Mol Brain Res. 64. 41-51 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nishimura M.: "Expression of norepinephrine and serotonin transporter mRNAs in the rat superior cervical ganglion"Mol Brain Res. 67. 82-86 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ito Y.: "Comparison between the decrease of dopamine transporter and that of L-DOPA uptake for detection of early to advanced stage of Parkinson's disease in animal models"Synapse. 31. 178-185 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 島田昌一: "味覚受容体遺伝子"脳21. 2. 304-308 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ugawa S.: "Receptor that leaves a sour taste in the mouth." Nature. 395. 555-556 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nishimura M.: "MK-801 blocks monoamine transporters expressed in HEK cells." FEBS Lett. 423. 376-380 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Okada T.: "Assessment of affinities of beta-CIT, beta-CIT-FE, and beta-CIT-FP for monoamine transporters permanently expressed in cell lines." Nucl Med Biol. 25. 53-58 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nonaka M.: "Increased transcription of glutamate-aspartate transporter (GLAST/GluT-1)mRNA following kainic acid-induced limbic seizure." Mol Brain Res. 55. 54-60 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Okada T.: "Tetrahydropapaveroline and its derivatives inhibit dopamine uptake through dopamine transporter expressed in HEK293 cells." Neurosci Res. 30. 87-90 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kitamura H.: "Inhibition of myo-inositol transport causes acute renal failure with selective medullary injury in the rat." Kidney Int. 53. 146-153 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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