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New methods for gene identification based on genomic analysis and their application for molecular breeding in plants.

Research Project

Project/Area Number 10554048
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section展開研究
Research Field 遺伝
Research InstitutionNational Institute for Basic Biology

Principal Investigator

IIDA Shigeru  National Institute for Basic Biology, Proffessor, 基礎生物学研究所, 教授 (30012777)

Co-Investigator(Kenkyū-buntansha) TANAKA Yoshikazu  Institute for Fundamental Research, Suntory Ltd., Senior researcher, 基礎研究所, 主任研究員
OZEKI Yoshihiro  Tokyo University of Agricultureand Technology, Associate professor, 工学部・生命工学科, 助教授 (50185592)
INAGAKI Yoshishige  National Institute for Basic Biology, Research associate, 基礎生物学研究所, 助手 (50280764)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2000: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥7,000,000 (Direct Cost: ¥7,000,000)
Keywordsanthocyanin biosynthesis gene / carnation / floricultural plants / gene identification / genome analysis / molecular breeding / the common morning glory / the Japanese morning glory
Research Abstract

The Japanese morning glory (Ipomoea nil) displaying blue flowers has an extensive history of genetic and physiological studies. A number of its spontaneous mutants related to the colors and shapes of the flowers and leaves have been isolated. Several lines of evidence indicate that an En/Spm-related transposable element Tpn1 and its relatives are major sources of these spontaneous mutations. We have developed a new method to identify a gene tagged by insertion of Tpn1 and its relatives, which we named Simplified Transposon Display (STD). Subsequently, we have successfully applied a purple-mutable allele conferring purple flowers with blue spots and sectors. The purple gene identified by STD was found to encode a vacuolar Na+/H+ exchanger that is responsible for increase vacuolar pH for blue flower coloration. We have also characterized carnation varieties and succeeded to identify new Ac/Ds-related transposable elements within the CHI and DFR senes for anthocyanin pigments biosynthesis. The integration of the transposable elements into these genes result in the altered pigmentation phenotypes. We were also able to generate new torenia varieties displaying pale flowers by introducing the CHS or DFR cDNA sequences.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] S.Fukada-Tanaka: "Colour-enhancing protein in blue petals."Nature. 407. 581 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Nakayama: "Aureusidin synthase : A polyphenol oxidase homolog responsible for flower coloration."Science. 290. 1163 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Yoshimoto: "Isolation and Characterization of a cDNA for phenylalanine ammonia-lyase (PAL) from Dianthus caryophyllus (carnation)"Plant Biotech.. 17. 325-329 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Fukada-Tanaka: "Simplified transposon display (STD): a new procedure for isolation of a gene tagged by a transposable element belonging to the Tpn1 family in the Japanese morning glory."Plant Biotech.. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] A.Hoshino: "Gene duplication and mobile genetic elements in the morning glories."Gene. 265. 1-10 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Fukada-Tanaka, et al.: "Colour-enhancing protein in blue petals."Nature. 407. 581 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Nakayama, et al.: "Aureusidin synthase : A polyphenol oxidase homolog responsible for flower coloration."Science. 290. 1163 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Yoshimoto, et al.: "Isolation and Characterization of a cDNA for phenylalanine ammonia-lyase (PAL) from Dianthus caryophyllus (carnation)"Plant Biotechnology. 17. 325-329 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Fukada-Tanaka, et al.: "Simplified transposon display (STD) : a new procedure for isolation of a gene tagged by a transposable element belonging to the Tpn1 family in the Japanese morning glory."Plant Biotechnology. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] A.Hoshino, et al.: "Gene duplication and mobile genetic elements in the morning glories."Gene. 265. 1-10 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Fukada-Tanaka: "Colour-enhancing protein in blue petals."Nature. 407. 581 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Nakayama: "Aureusidin synthase : A polyphenol oxidase homolog responsible for flower coloration."Science. 290. 1163 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Yoshimoto: "Isolation and Characterization of a cDNA for phenylalanine ammonia-lyase (PAL) from Dianthus caryophyllus (carnation)"Plant Biotech.. 17. 325 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] S.Fukada-Tanaka: "Simplified transposon display (STD) : a new procedure for isolation of a gene tagged by a transposable element belonging to the Tpn1 family in the Japanese morning glory."Plant Biotech.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] A.Hoshino: "Gene duplication and mobile genetic elements in the morning glories."Gene. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y. Inagaki: "Genomic organization of the genes encoding dihydroflavonol 4-reductase for flower pigmentation in the Japanese and common morning glories"Gene. 226. 181-188 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S. Takahashi: "Capturing of a genomic HMG domain sequence by an En/Spm related transposable element Tpn1 in the Japanese morning glory"Mol. Gen. Genet.. 261. 447-451 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S. Iida: "Floricultural traits and transposable elements in the Japanese and common morning glories"Annal. New York Acad. Sci.. 870. 265-274 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M. Yamazaki: "Molecular cloning and biochemical characterization of a novel anthocyanin 5-O-glucosyltransferase by mENA differential display for plant forms regarding anthocvanin"J. Biol. Chem.. 274. 7405-7511 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K. Saito: "Direct evidence for anthocyanin synthase as a 2-oxoglutarate-dependent oxygenase : molecular cloning and functional expression of cDNA from a red forma of Perilla frutescens"Plant J.. 17. 181-189 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Akashi: "Molecular cloning and biochemical characterization of a novel cytochrome P450, flavone synthase II, that catalyzes direct conversion of flavanones to flavones"Plant Cell Physiol.. 40. 1182-1186 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y.Habu: "AFLP(amplified restriction fragment length polymorphism)-based mRNA fingerprinting." Plant Biotech.15. 249-251 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Habu: "Molecular characterization of the mutable flaked allele for flower variegation in the common morning glory." Plant J.16. 371-376 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Inagaki: "Genomic organization of the genes encoding dihydroflavonol 4-reductase for flower pigmentation in the Japanese and common morning glories." Gene. 226. 181-188 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Iida: "Floricultural traits and transposable elements in the Japanese and common morning glories." Annal.New York Acad.Sci.(in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Mato: "Isolation and characterization of a cDNA clone of UDP-galactose:flavonoid 3-O-galactosyltransferase(UF3GaT)expressed in the seeding of Vigna mungo." Plant Cell Physiol.39. 1145-1155 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Fujiwara: "cDNA cloning,gene expression and subcellular localization of anthocyanin 5-aromatic acyltransferase from Gentiana triflora" Plant J.16. 421-431 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 飯田 滋: "植物の生長、遺伝子から何が見えるか。" クバプロ, 169 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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