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Development of Detection low level of pthogens in water by genetic biotechnology

Research Project

Project/Area Number 10555189
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Civil and environmental engineering
Research InstitutionThe University of Tokyo

Principal Investigator

OHGAKI Shinichiro  Univ. of Tokyo, Dept. of Urban Eng., Professor, 大学院・工学系研究科, 教授 (20005549)

Co-Investigator(Kenkyū-buntansha) KAMIKO Naoyuki  Ibaraki Univ., Dept. of Civil eng., Asoc. Prof., 工学部, 助教授 (70251345)
YANO Kazuyosi  Tokyo Metropolitan Sanitary Inst., Researcher, 環境保健部, 主任研究員
FURUMAI Hiroaki  Univ. of Tokyo, Dept. of Urban Eng., Professor, 大学院・工学系研究科, 教授 (40173546)
KATAYAMA Hiroyuki  Univ. of Tokyo, Dept. of Urban Eng., Research Associate, 大学院・工学系研究科, 助手 (00302779)
OTAKI Masahiro  Ochanomizu Univ., Dept. of Human Environmental Sci., Asoc., 人間文化研究科, 助教授 (70272367)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 1999: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1998: ¥4,400,000 (Direct Cost: ¥4,400,000)
KeywordsPCR / virus / water microbiology / ozonation / concentration / negatively-charged membrane / disinfection / 検出 / ゲノム / 酸洗浄
Research Abstract

Management of enteric viruses in water is one of the important issues for the safety of drinking water. PCR is one of the feasible monitoring methods of viruses in water environment, because it requires for detection less than 6 hours and no cultivation of pathogenic viruses with high sensitivity. However established virus concentration method from water are not suitable for detection by RT-PCR. A Virus concentration method with negatively-charged membrane using acid rinse and alkaline elution was developed and applied to F specific RNA coliphage QB and poliovirus 1 as model viruses. The concentration of QB and poliovirus were determined by quantitative TaqMan RT-PCR or plaque assay. Various pH condition were tested for resistance of virus and its genomic RNA, and applied to the virus concentration method. Acid rinse procedure was successfully increased the recovery yields, which was also confirmed by plaque assay. Ozonation was applied as disinfection of viruses and false positive result was obtained. From the quantitative analysis of inactivation, mechanism of inactivation by ozonation was explained by a simple model.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 大垣眞一郎: "塩素代替消毒技術の現状と今後の課題"水環境学会誌. 21. 560-565 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 片山浩之: "陰電荷膜を用いたファージ遺伝子の濃縮法の開発"日本水環境学会年会講演集. 498 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 嶋崎明寛: "水中RNAウイルスの陰電荷膜を用いた濃縮法への酸洗浄の導入"環境工学研究フォーラム講演集. 36. 64-66 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 嶋崎明寛: "水中のポリオウイルス濃縮法の定量的RT-PCR法による回収率の測定"日本水環境学会年会講演集. 34. 321 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 原田宣男: "大腸菌ファージQβに対するオゾン消毒効果のPCR法による定量評価"日本水環境学会年会講演集. 34. 435 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shinichiro Ohggaki: "Current Status of Disinfection"Jour. of Japan Society of Water Environment. 21. 560-565 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Katayama Hiroyuki: "Development of Concentration Method for Coliphage Genome Using Negatively Charged Membrane"Proc. of Japan Society of Water Environment.. 33. 498 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Akihiro SHIMASAKI: "A Virus Concentration Method of RNA Viruses in Water Using Negatively-Charged Membrane with Acid Rinse Procedure"Proc. of Environmental Research Forum. 36. 64-66 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Akihiro SHIMASAKI: "Recovery Yields of a Poliovirus Concentration Method Determined by Quantitative RT-PCR"Proc. of Japan Society of Water Environment. 34. 321 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nobuo HARATA: "The Quantitative Evaluation of Ozone Disinfection to Phage Qb by the PCR Method"Proc. of Japan Society of Water Environment. 34. 435 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 嶋崎 明寛: "水中RNAウイルスの陰電荷膜を用いた濃縮法への酸洗浄工程の導入"第36回環境工学フォーラム講演集. 64-66 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 嶋崎 明寛: "水中のポリオウイルス濃縮法の定量的RT-PCR法による回収率の測定"第34回日本水環境安全年会講演習. (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 片山 浩之: "陰電荷膜を用いたファージ遺伝子の濃縮法の開発" 日本水環境学会年会講演集. 第33回. (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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