| Project/Area Number |
10555286
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
生物・生体工学
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| Research Institution | Niigata University |
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Principal Investigator |
TANIGUCHI Masayuki Niigata University, Faculty of Engineering Professor, 工学部, 教授 (00163634)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Takaaki Niigata University, Faculty of Engineering Associate Professor, 工学部, 助教授 (00217043)
IIDA Takamitsu Niigata University, Faculty of Engineering Professor, 工学部, 教授 (00281006)
HOSHINO Kazuhiro Toyama University Faculty of Engineering Associate Professor, 工学部, 助教授 (20222276)
HOJO Kenichi Meiji Milk Products Co.Central Research Institute Researcher, 中央研究所, 研究員
KANEKO Tsutomu Meiji Milk Products Co.Central Research Institute Research Manager, 中央研究所, 課長(研究職)
北条 研一 明治乳業株式会社, 中央研究所, 研究員
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥12,700,000 (Direct Cost: ¥12,700,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1999: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1998: ¥7,000,000 (Direct Cost: ¥7,000,000)
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| Keywords | Propionibacterium / Bifidobacterium / Co-culture / Biorecactor / Growth factor / Probiotics |
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| Research Abstract |
1. The concentration and the productivity per time of bifidogenic growth stimulator were increased by using a co-culture system for a homolactic acid bacterium and a propionic acid bacterium, a culture system with microfiltration, a fed-batch culture system with feed back control of carbon source concentration, and a culture system with microfiltration and feed back control of carbon source concentration.
2. A novel bioreactor system with two fermentors and microfiltration modules was developed for efficient production bifidobacterial cells by a co-culture of Propionibacterium freudenreichii and Bifidobacterium adolescentis as well as clarification of interactions between the two bacteria.
3. The proposed co-culture system allowed the successful exchange of culture medium between two fermentors. When P.freudenreichii and B.adolescentis, B.longum, or B.breve were cultivated individually in the new co-culture system, the amount of bifidobacterial cells produced was 1.2-1.6 fold as high as that with batch culture.
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