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Bactericidal principle of the fermented aqueous extract of Stevia rebaudiana Bertoni and a possible use of the Stevia extract as a feed supplement for prevention of the digestive tract infections

Research Project

Project/Area Number 10556014
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 応用微生物学・応用生物化学
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

KAMIO Yoshiyuki  Graduate School of Agricultural Science, Tohoku University, Professor, 大学院・農学研究科, 教授 (00109175)

Co-Investigator(Kenkyū-buntansha) KANEKO Jun  Graduate School of Agricultural Science, Tohoku University, Research Associate, 大学院・農学研究科, 助手 (30221188)
ABE Naoki  Graduate School of Agricultural Science, Tohoku University, Research Associate, 大学院・農学研究科, 助手 (90202671)
TOMITA Toshio  Graduate School of Agricultural Science, Tohoku University, Assistant Professor, 大学院・農学研究科, 助教授 (00126129)
SHIRAISHI Hiroyuki  Microbiology Division, Miyagi Prefectural Institute of Public Health and Environment, Head of Microbiology Division, 微生物部, 部長(研究職)
SATO Naohiko  JBB Stevia Laboratory, Head, 社長(研究職)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 1999: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1998: ¥8,500,000 (Direct Cost: ¥8,500,000)
KeywordsStevia rebaudiana Bertoni / bactericidal substances / fermentes extract / domestic animals / bacterial infection / feed supplement / digestive tract / 予防 / 殺菌感染
Research Abstract

We have previously shown that a fermenteds extract from the Paraguayan composite plant, Stevia rebaudiana Bertoni has strong bactericidal activity towards a wide range of food-borne pathogenic bacteria, but it shows no significant killing activity to Bifidobacteria and Lactobacilli. Here we studied bactericidal principle of the fermented extract, and tested the extract for possible use as a feed supplement for prevention of the intestinal tract infections of domestic animals.
1. Fermented aqueous extract of Stevia rebaudiana Bertoni was fractionated by the partition with ethyl acetate, followed by the high performance column chromatography using an ODS (CィイD218ィエD2) column. Two bactericidal fractions and one enhancer fraction were obtained. Viability of E. coli was reduced from 10ィイD19ィエD1 cfu/ml to <10ィイD13ィエD1 cfu/ml when incubated with the bactericidal fractions at 37℃ for 1h. One bactericidal fraction has he maximal absorption at 280 nm, and it was sensitive to light irradiation. The other bactericidal fraction was identified as lactic acid.
2. In the laboratory experiments, oral administration of the Stevia extract to the Listeria-infected mice decreased the numbers of viable bacteria in the intestinal tract from 10ィイD17ィエD1 cfu/ml to 10ィイD13ィエD1 cfu/ml within 3 days. Oral administration of the extract to the Salmonella-infected calves reduced viable bacteria in the intestinal tract and bacterial tissue invasion.
3. In the field experiments, oral administration of the Stevia extract was effective to the intestinal tract infections of piggys. Many of the animals with early-staged diarrhea were recovered within 3 days after the treatment, and their growth rate was normal. Oral administration of the diluted Stevia extract promoted growth of pigs, and the quality of the pork meat was evaluated high at the slaughter house.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 冨田敏夫、神尾好是、佐藤直彦: "食中毒菌に対する選択的殺菌剤"公開特許広報 特開平11-43443. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 冨田敏夫、佐藤直彦、佐藤實、白石廣行、竹内昌昭、神尾好是: "腸管出血性大腸菌及び他の食中毒起因菌に対するステビア発酵液の殺菌効果"日本細菌学雑誌. 53. 2039 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 冨田敏夫、佐藤直彦、佐藤*、荒井富雄、白石廣行、神尾好是: "腸管出血性大腸菌O157:H7及び他の食中毒起因菌に対するステビア発酵液の殺菌効果"日本農芸化学会誌(大会講演要旨集). 72. 126 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshio TOMITA, Yoshiyuki Kamio, and Naohiko SATO: "Selective bactericide agains food-borne pathogenic bacteria"Japanese Patent Opened 11-43443. (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshio TOMITA, Naohiko SATO, Minoru SATO, Hiroyuki SHIRAISHI, Masayuki TAKEUCHI, and Yoshiyuki KAMIO: "Bactericidal activity of a fermented extract of Stevia rebaudiana Bertoni towards enterohaemorrhagic Escherichiia colli and other food-borne pathogenic bacteria"Japanese Journal of Bacteriology. Vol. 53, No. 1. 178 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshio TOMITA, Naohiko SATO,: "Antibacterial effect of a fermented extract of Stevia rebaudiana Bertoni on the enterohaemorrhagic Escherichiia colli O157:H7 and the other food-borne pathogenic bacteria"Nippon Nogeikagaku Kaishi, (Proceedings of the Annual Meeting 1998). Vol. 72. 126 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 富田敏夫、神尾好是、佐藤直彦: "食中毒菌に対する選択的殺菌剤"公開特許広報 特開平11-43443. (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 冨田敏夫、神尾好是、佐藤直彦: "食中毒菌に対する選択的殺菌剤" 公開特許広報 特開平11-43443. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 冨田敏夫、佐藤直彦、佐藤 實、白石廣行、竹内昌昭、神尾好是: "腸管出血性大腸菌及び他の食中毒起因菌に対するステビア発酵液の殺菌効果" 日本細菌学雑誌. 53・1. 2039(頁) (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 冨田敏夫、佐藤直彦、佐藤 實、荒井富雄、白石廣行、神尾好是: "腸管出血性大腸菌O157:H7及び他の食中毒起因菌に対するステビア発酵液の殺菌効果" 日本農芸化学会誌. (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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