| Project/Area Number |
10556015
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OHTA Akinori Graduate school of Agricultural and Life Sciences Univ. Tokyo, Professor, 大学院・農学生命科学研究科, 教授 (30125885)
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| Co-Investigator(Kenkyū-buntansha) |
TERASAWA Masato Mitsubishi Chemicals, LTD, Dept. Biochem., Investigator, 筑波研究所, 主任研究員
YUKAWA Hideaki LITE, Dept. Mol. Microbiol., Principal, Investigator, 微生物分子機能研究室, 主席研究員
HORIUCHI Hiroyuki Graduate school of Agricultural and Life Sciences, Univ. Tokyo, Assoc. Professor, 大学院・農学生命科学研究科, 助教授 (00209280)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 1999: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1998: ¥9,700,000 (Direct Cost: ¥9,700,000)
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| Keywords | endoplasmic reticulum / yeast / Candida maltosa / cytochrome P450Alk / Yarrowia lipolytica / dicarboxylic acid / cytochrome P450 reductase / heterologous expression |
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| Research Abstract |
The aim of this research was to construct microbial systems which produce oxidized hydrocabons, such as long chain dicarboxylic acids. For this purpose, we cloned various genes which are encoding cytochromes P450 with different specificities, but of CYP52 family members. Those were 8 ALK genes from the yeast Candida maltosa and also 8 YlALK genes from Yarrowia lipolytica. The product of ALK1 gene that is prominently induced and expressed in C. maltosa oxidized very end of n-alkanes, whereas ALK5 gene product also oxidized omega position of fatty acids. ALK7 and ALK8 gene products only oxidize fatty acids. These gene were functionally expressed in heterologous yeast Saccharomyces cerevisiae. The eight cytochromes P450ALK from Y. lipolytica form a different CYP52 sub family. Among the products of YlALK genes, Ylalklp seemed principally to oxidize n-decane and Ylalk2p had some activity to n-hexadecane.
These genes encoding different kind of cytochromes P450, properties of which have been elucidated by our current research, thus can be introduced into a new host and be utilized to construct a new production system of oxidized alkanes.
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