| Project/Area Number |
10556016
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | Nagoya University |
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Principal Investigator |
OSAWA Toshihiko Nagoya University, Graduate School of Bioagricultural Sciences, Ph.D Professor, 大学院・生命農学研究科, 教授 (00115536)
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| Co-Investigator(Kenkyū-buntansha) |
UCHIDA Koji Nagoya University, Graduate School of Bioagricultural Sciences, Ph.D Assoc. Prof., 大学院・生命農学研究科, 助教授 (40203533)
程 栄助 日本老化制御研究所, 研究員
森光 康次郎 名古屋大学, 農学部, 助手 (00244533)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 1999: ¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1998: ¥8,000,000 (Direct Cost: ¥8,000,000)
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| Keywords | Oxidative Stress / Monoclonal Antibody / Hexanoyl Lysine / Methyl Glyoxal / Acrolein / Dityrosine / Lipid Peroxidations Products / DNA Oxidative Modification / メチルグリオキサール / 糖尿病バイオマーカー |
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| Research Abstract |
Oxidative stress may cause free radical reactions to produce deleterious modifications in membranes, proteins, enzymes and DNA. Age-related diseases such as cancer, atherosclerosis and diabetes are supposed to be correlated with oxidative stress although the detailed mechanisms are still unclear. Our research group has been involved in developing novel ELISA methods by application of immnochemistry. We have developed many types of monoclonal and polyclonal antibodies which are specific to oxidatively damaged DNA base such as 8-OH-deoxyguanosine and lipid peroxidation products including malondialdehyde, 4-hydroxynonenal and aclorein etc. We have also been involved in isolation and structure determination of oxidatively modified proteins by linoleic acid hydroperoxide. By the chemical investigation, we have determined the structure of epitope which is specific to early stage lipid peroxidation products as hexanoyl lysine type compounds. We have succeeded in obtaining both monoclonal and polyclonal antibodies specific for hexanoyl lisine structure as epitope. Recently, we also obtained polyclonal antibody specific to dityrosine as the new biomarker for one of the oxidatively damaged proteins by myropexidase. Using these monoclonal and polyclonal antibodies, we are trying to establish ELISA methods.
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