| Project/Area Number |
10556033
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
林学
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
TOGASHI Katsumi Hiroshima Univ., Fac. Integrated Arts and Sciences, Professor, 総合科学部, 教授 (30237060)
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| Co-Investigator(Kenkyū-buntansha) |
HONDA Keiichi Hiroshima Univ., Fac. Integrated Arts and Sciences, Professor, 総合科学部, 教授 (00238809)
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| Project Period (FY) |
1998 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Monochamus alternatus / Japanese pine sawyer / oviposition / oviposition deterrent / frass / spermathecal gland / pinp wilt disease / Bursaphelenchus xylophilus |
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| Research Abstract |
Monochamus alternates females deposit jelly-like substance near the eggs just after opposition. The substance deterred the conspecific females from opposition. Colorless to pale yellow, transparent liquid was present in the long spermathecal gland of living females and changed to amber, transparent, elastic substance after females died, which greatly resembled jelly-like substance in opposition scars. Methanol extract of spermathecal gland deterred females from opposition. Section showed that the liquid in spermathecal gland was found in bursa copulatrix. The facts showed that spermathecal gland produced the oviposition deterrents. When methanol extract of spermathecal gland was fractionated with acetone and methanol, methanol-insoluble fraction showed an oviposition-deterring effect. Treatment with cold methanol after concentration divided the active fraction into methanol-insoluble fraction including protein and methanol-soluble fraction. Neither of the two fractions had oviposition-
… More
deterring effect. A mixture of them could not recover the activity. Methanol extract of fresh larval frass deterred females from opposition. The extract was fractionated with benzene, chloroform, acetone, and methanol. As only the benzene-soluble fraction showed an oviposition-deterring effect, it was fractionated into six fractions by successive silica gel column chromatography. Hexane-soluble fraction of them had an oviposition-deterring effect. The hexanesoluble fraction was partitioned into five fractions by column chromatography. As the resulting benzene soluble and methanol-soluble fractions showed an oviposition-deterring effect, both fractions were subjected to TLC analysis, where 7 and 3 spots were observed, respectively. Substances of one spot from benzene fraction showed an oviposition-deterring effect. GC and GC-MS revealed the presence of 6 compounds. Acetophenone thus identified showed the greatest oviposition-deterring effect among the compounds although there was no statistical significance. Less
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